目的:基于(2,3-二油酰基-丙基)-三甲胺(DOTAP)聚乙二醇-聚己内酯(MPEG-PCL)制备阳离子胶束DMP,并对其表征以及不用浓度的血清转染进行考察。方法:用旋转蒸发仪制备阳离子胶束DMP,用马尔文激光粒度仪对粒径和zeta电位进行了测定,用凝胶...目的:基于(2,3-二油酰基-丙基)-三甲胺(DOTAP)聚乙二醇-聚己内酯(MPEG-PCL)制备阳离子胶束DMP,并对其表征以及不用浓度的血清转染进行考察。方法:用旋转蒸发仪制备阳离子胶束DMP,用马尔文激光粒度仪对粒径和zeta电位进行了测定,用凝胶阻滞来确定阳离子胶束DMP完全阻滞RNA的比例,用流式细胞仪定量检测阳离子胶束DMP在含有不同浓度梯度的血清的转染效率。结果:通过对阳离子胶束DMP的制备工艺的筛选,制备出阳离子胶束DMP,测得粒径为144.8 nm,PDI=0.183,zeta电位46.4 m V,带有正电荷。凝胶阻滞实验结果显示,当DMP与Scramble si RNA N/P≥30时能够完全阻滞。体外转染实验显示,在含有0%,10%,20%,30%的血清中,DMP/FAM si RNA复合物对C26结肠癌细胞的转染效率分别为85.47±1.01%,81.57±2.04%,75.29±1.20%,71.64±1.59%。结论:阳离子胶束DMP具有良好的粒径和电位,其转染效率不受血清的影响,在不同浓度的血清转染中均具有较高的转染效率,并且具有较低的细胞毒性,可以作为一种安全有效的si RNA载体。展开更多
The effect of cationic micelles of cetyltrimethylammonium bromide (CTAB) on the interaction of chromium dipeptide complex ([Cr(Ⅲ)-Gly-Gly]2+) with ninhydrin under varying conditions has been investigated. The rates o...The effect of cationic micelles of cetyltrimethylammonium bromide (CTAB) on the interaction of chromium dipeptide complex ([Cr(Ⅲ)-Gly-Gly]2+) with ninhydrin under varying conditions has been investigated. The rates of the reaction were determined in both water and surfactant micelles in the absence and presence of various organic and inorganic salts at 70 ℃ and pH 5.0. The reaction followed first- and fractional-order kinetics with respect to [Cr(Ⅲ)- Gly-Gly2+] and [ninhydrin]. Increase in the total concentration of CTAB from 0 to 40×10-3 mol·dm-3 resulted in an increase in the pseudo-first-order rate constant (kψ) by a factor of ca 3. Quantitative kinetic analysis of kψ-[CTAB] data was performed on the basis of the pseudo-phase model of the micelles. As added salts induce structural changes in micellar systems that may modify the substrate-surfactant interactions, the effect of some inorganic (NaBr, NaCl, Na2SO4) and organic (NaBenz, NaSal, NaTos) salts on the rate was also explored. It was found that the tightly bound counterions (derived from organic salts) were the most effective.展开更多
文摘目的:基于(2,3-二油酰基-丙基)-三甲胺(DOTAP)聚乙二醇-聚己内酯(MPEG-PCL)制备阳离子胶束DMP,并对其表征以及不用浓度的血清转染进行考察。方法:用旋转蒸发仪制备阳离子胶束DMP,用马尔文激光粒度仪对粒径和zeta电位进行了测定,用凝胶阻滞来确定阳离子胶束DMP完全阻滞RNA的比例,用流式细胞仪定量检测阳离子胶束DMP在含有不同浓度梯度的血清的转染效率。结果:通过对阳离子胶束DMP的制备工艺的筛选,制备出阳离子胶束DMP,测得粒径为144.8 nm,PDI=0.183,zeta电位46.4 m V,带有正电荷。凝胶阻滞实验结果显示,当DMP与Scramble si RNA N/P≥30时能够完全阻滞。体外转染实验显示,在含有0%,10%,20%,30%的血清中,DMP/FAM si RNA复合物对C26结肠癌细胞的转染效率分别为85.47±1.01%,81.57±2.04%,75.29±1.20%,71.64±1.59%。结论:阳离子胶束DMP具有良好的粒径和电位,其转染效率不受血清的影响,在不同浓度的血清转染中均具有较高的转染效率,并且具有较低的细胞毒性,可以作为一种安全有效的si RNA载体。
文摘The effect of cationic micelles of cetyltrimethylammonium bromide (CTAB) on the interaction of chromium dipeptide complex ([Cr(Ⅲ)-Gly-Gly]2+) with ninhydrin under varying conditions has been investigated. The rates of the reaction were determined in both water and surfactant micelles in the absence and presence of various organic and inorganic salts at 70 ℃ and pH 5.0. The reaction followed first- and fractional-order kinetics with respect to [Cr(Ⅲ)- Gly-Gly2+] and [ninhydrin]. Increase in the total concentration of CTAB from 0 to 40×10-3 mol·dm-3 resulted in an increase in the pseudo-first-order rate constant (kψ) by a factor of ca 3. Quantitative kinetic analysis of kψ-[CTAB] data was performed on the basis of the pseudo-phase model of the micelles. As added salts induce structural changes in micellar systems that may modify the substrate-surfactant interactions, the effect of some inorganic (NaBr, NaCl, Na2SO4) and organic (NaBenz, NaSal, NaTos) salts on the rate was also explored. It was found that the tightly bound counterions (derived from organic salts) were the most effective.
