Objective To explore the metabolic differences between dry eye patients with Qi-Yin defi-ciency and heat stagnation in liver meridian patterns,and clarify their metabolic characteris-tics.Methods Patients with dry eye...Objective To explore the metabolic differences between dry eye patients with Qi-Yin defi-ciency and heat stagnation in liver meridian patterns,and clarify their metabolic characteris-tics.Methods Patients with dry eye who were treated in the Ophthalmology Ward and Outpatient Department of the First Hospital of Hunan University of Chinese Medicine from October 1,2020,to October 30,2021 were enrolled as the research participants in the study.They were assigned to two groups based on traditional Chinese medicine(TCM)syndrome types:heat stagnation in liver meridian pattern group and Qi-Yin deficiency pattern group.Healthy vol-unteers who underwent health check-ups in the Health Management Department were in-cluded as healthy group following the random number table method.The tears of the pa-tients and the healthy volunteer participants were tested by high-performance liquid chro-matography-mass spectrometry(LC-MS).The differential metabolites were screened out by multivariate statistical analysis,and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment was performed on the differential metabolites.Finally,the association analysis of differential proteins and metabolites was conducted to verify and supplement the metabolites.Results A total of 32 dry eye patients were enrolled,including 16 cases with heat stagnation in liver meridian pattern and 16 cases with Qi-Yin deficiency pattern.Fourteen healthy volun-teers were included as healthy group.There were no significant differences in baseline char-acteristics among the three groups(P>0.05).A total of 412 biomarkers were determined in Qi-Yin deficiency pattern group,mainly including lipids,lipid-like molecules,organic acids and their derivatives,organic heterocyclic compounds,and nucleosides and their analogues.For heat stagnation in liver meridian pattern group,112 metabolites were determined,main-ly including organic acids and their derivatives,lipids,and lipid-like molecules.The KEGG enrichment results of pathways and the relative content analysis of differential markers de-monstrate that purine metabolism and caffeine metabolism pathways are common metabol-ic characteristics of all dry eyes.Among them,deoxyinosine monophosphate(dIMP)and 2-(formamido)-N1-(5-phospha-D-ribosyl)acetamidine can serve as their biomarkers.The main characteristics of Qi-Yin deficiency syndrome pattern were the significant enhancement of metabolic pathways such as lysine degradation,ovarian steroidogenesis,cholesterol metabolism,pyrimidine metabolism,and bile secretion(P<0.05).Dry eye associated with the heat stagnation in liver meridian pattern is mainly characterized by inhibition of the valine,leucine,and isoleucine biosynthesis pathways(P<0.05).Conclusion Metabolomics can be used as an effective basis for TCM syndrome classification.Different patterns of dry eye syndrome exhibit typical characteristics in the types and concen-trations of metabolites,which correspond to the syndrome classification in TCM.This study initially confirms the rationality of TCM syndrome classification and provides significant ref-erence for the mechanism of dry eye and drug development.展开更多
A potential 4.2 V cathode material LiVPO4F for lithium batteries was prepared by two-step reaction method based on a carbon-thermal reduction （CTR） process. Firstly, V2O5, NH4H2PO4 and acetylene black are reacted un...A potential 4.2 V cathode material LiVPO4F for lithium batteries was prepared by two-step reaction method based on a carbon-thermal reduction （CTR） process. Firstly, V2O5, NH4H2PO4 and acetylene black are reacted under an Ar atmosphere to yield VPO4. The transition-metal reduction is facilitated by the CTR based on C→CO transition. These CTR conditions favor stabilization of the vanadium as V^3＋ as well as leaving residual carbon, which is useful in the subsequent electrode processing. Secondly, VPO4 reacts with ElF to yield LiVPO4F product. The property of the LiVPO4F was investigated by X-ray diffractometry （XRD）, scanning electron microscopy （SEM） and electrochemical measurement. XRD studies show that LiVPO4F synthesized has triclinic structure（space group p I ）, isostructural with the naturally occurring mineral tavorite, EiFePO4-OH. SEM image exhibits that the particle size is about 2μm together with homogenous distribution. Electrochemical test shows that the initial discharge capacity of LiVPO4F powder is 119 mA·h/g at the rate of 0.2C with an average discharge voltage of 4.2V （vs Ei/Li^＋）, and the capacity retains 89 mA·h/g after 30 cycles.展开更多
The sunny-shady slopes effect is a phenomenon that impacts the temperature distribution of high-speed railway subgrades,resulting in uneven frost heaving deformation on the subgrade surface,which in turn causes static...The sunny-shady slopes effect is a phenomenon that impacts the temperature distribution of high-speed railway subgrades,resulting in uneven frost heaving deformation on the subgrade surface,which in turn causes static irregularity in the slab track.Based on the hydraulics theory,a thermal-hydro-mechanical(THM)coupled model of frozen soil is established and verified.We explore the process and characteristics of the temperature field and deformation of soil during the freezing process of high-speed railway subgrades and analyze the track irregularity variation law of China Railway Track SystemⅢslab tracks under uneven frost heaving deformation.The results show that,because the left and right slopes of high-speed railway subgrade are exposed to different amounts of solar radiation,which is the key factor causing uneven frost heaving of subgrade.Different strike angles cause changes in temperature of the subgrade’s upper part and the frost heaving amount on the surface,leading to differences in the deformation of the slab track structure:Increased strike angle weakens the rail level irregularity of the down line and marginally increases the rail level irregularity of the up line,and these become consistent in north-south directions.Therefore,when selecting railway lines in seasonal frozen areas,the west-east direction should be avoided to prevent the extremes in sunny-shady slopes effect on subgrades.展开更多
Warming yang is one of the most important therapeutic methods for treating diseases. In Peaceful Holy Benevolent Prescriptions (太平圣惠方 a collection of more than ten thousand formulas) written by Dr. Wang Huaiyin ...Warming yang is one of the most important therapeutic methods for treating diseases. In Peaceful Holy Benevolent Prescriptions (太平圣惠方 a collection of more than ten thousand formulas) written by Dr. Wang Huaiyin (王怀隐) et al in the Song Dynasty in 992 AD, more than one hundred formulas for treating diabetes are all complied with the principles of supplementing qi and nourishing yin. Dr. Liu Hejian (刘河间) in the period of Jin and Yuan Dynasty advocated that it should be treated with the principles of clearing away pathogenic heat and fire, and supplementing qi and nourishing yin. Dr. Huang Kunzai (黄坤载) in the Qing Dynasty held that diabetes should be treated by supplementing qi and nourishing yin.展开更多
Animal performance is affected by high air temperature and it is known that shade reduces the absorption of radiant temperature, and water for immersion facilitates heat loss. This study intends to find preferences of...Animal performance is affected by high air temperature and it is known that shade reduces the absorption of radiant temperature, and water for immersion facilitates heat loss. This study intends to find preferences of resources that contribute for the well-being of cattle and how they alterdaily behaviour. During summer, six Caracu and six Red Angus bulls were submitted to two different treatments： availability of artificial shade and water for immersion and availability of water for immersion. The categories observed were： positions （in the sun, under the shade, in the water）, posture （standing, lying down） and activities （grazing, ruminating leisure）. The behavioural patterns were recorded using the focal sampling method every 15 minutes （from 6：00 a.m. to 6：00 p.m.）. When shade and water for immersion coexists, cattle in this study prefer shade to avoid solar radiation. Both breeds had remained more time grazing, followed by ruminating in the Caracu breed, and by resting in the Red Angus breed. The Caracu breed had presented clear preference for the shade resource, but that fact was not always observed in the Red Angus breed. In hot climates, resources for defence against heat load, as shade and water for immersion improve the well-being of cattle.展开更多
The heating area of the material is large and the thermal efficiency is high,but it is n ecessary to control the suitable drying temperature ofdiffere nt medici nal materials to preserve the effective in gredients.D i...The heating area of the material is large and the thermal efficiency is high,but it is n ecessary to control the suitable drying temperature ofdiffere nt medici nal materials to preserve the effective in gredients.D iff ere nt kinds of Chinese medicine need different drying conditions to fulfill good drying requirements.Natural drying in the shade is one of the traditional drying methods,which takes a long time and is easily affected by the weather.The water volatilizes slowly.It is prone to mildew and discoloration during the drying process.However,it can better preserve the volatile oil components of Chinese medicine.The hot-air drying machine has lower requirements.The medicinal materials have a large heating area and high thermal efficiency,but it is necessary to control the appropriate drying temperature of different medicinal materials in order to preserve the active ingredients of the medicinal materials;it is not suitable for medicinal materials that stick and bind easily.The microwave drying method possesses superiority in drying some valuable medicinal materials such as Ren she n(Radix et Rhizoma Ginseng)and Lurong(Co「nu Cervi Pantotrichum),and the effective ingredients are preserved at a high degree;it can also achieve the purpose of killing enzymes and protecting glycosides and have a good bactericidal effect,but it is not suitable for Chinese medicines containing heat-sensitive ingredients,because it will destroy most of the proteins,amino acids,and peptides of Chinese medicine and result in the loss of efficacy.The far-infrared drying method is suitable for drying thin-layer medicinal materials and is friendly to the environment.Freeze-drying can preserve the active ingredients very well and greatly retain the efficacy,but it has obvious limitations in preserving some Chinese medicinal materials that need to kill enzymes and protect glycosides;besides,the cost is relatively high and the drying time is long.展开更多
基金National Natural Science Foundation of China Project(82305037)Guiding Project for Innovative Clinical Medical Technology in Hunan Province(2021SK50809)Scientific Research Project of Hunan Administration of Traditional Chinese Medicine(D2022053).
文摘Objective To explore the metabolic differences between dry eye patients with Qi-Yin defi-ciency and heat stagnation in liver meridian patterns,and clarify their metabolic characteris-tics.Methods Patients with dry eye who were treated in the Ophthalmology Ward and Outpatient Department of the First Hospital of Hunan University of Chinese Medicine from October 1,2020,to October 30,2021 were enrolled as the research participants in the study.They were assigned to two groups based on traditional Chinese medicine(TCM)syndrome types:heat stagnation in liver meridian pattern group and Qi-Yin deficiency pattern group.Healthy vol-unteers who underwent health check-ups in the Health Management Department were in-cluded as healthy group following the random number table method.The tears of the pa-tients and the healthy volunteer participants were tested by high-performance liquid chro-matography-mass spectrometry(LC-MS).The differential metabolites were screened out by multivariate statistical analysis,and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment was performed on the differential metabolites.Finally,the association analysis of differential proteins and metabolites was conducted to verify and supplement the metabolites.Results A total of 32 dry eye patients were enrolled,including 16 cases with heat stagnation in liver meridian pattern and 16 cases with Qi-Yin deficiency pattern.Fourteen healthy volun-teers were included as healthy group.There were no significant differences in baseline char-acteristics among the three groups(P>0.05).A total of 412 biomarkers were determined in Qi-Yin deficiency pattern group,mainly including lipids,lipid-like molecules,organic acids and their derivatives,organic heterocyclic compounds,and nucleosides and their analogues.For heat stagnation in liver meridian pattern group,112 metabolites were determined,main-ly including organic acids and their derivatives,lipids,and lipid-like molecules.The KEGG enrichment results of pathways and the relative content analysis of differential markers de-monstrate that purine metabolism and caffeine metabolism pathways are common metabol-ic characteristics of all dry eyes.Among them,deoxyinosine monophosphate(dIMP)and 2-(formamido)-N1-(5-phospha-D-ribosyl)acetamidine can serve as their biomarkers.The main characteristics of Qi-Yin deficiency syndrome pattern were the significant enhancement of metabolic pathways such as lysine degradation,ovarian steroidogenesis,cholesterol metabolism,pyrimidine metabolism,and bile secretion(P<0.05).Dry eye associated with the heat stagnation in liver meridian pattern is mainly characterized by inhibition of the valine,leucine,and isoleucine biosynthesis pathways(P<0.05).Conclusion Metabolomics can be used as an effective basis for TCM syndrome classification.Different patterns of dry eye syndrome exhibit typical characteristics in the types and concen-trations of metabolites,which correspond to the syndrome classification in TCM.This study initially confirms the rationality of TCM syndrome classification and provides significant ref-erence for the mechanism of dry eye and drug development.
基金Project(50302016) supported by the National Natural Science Foundation of China
文摘A potential 4.2 V cathode material LiVPO4F for lithium batteries was prepared by two-step reaction method based on a carbon-thermal reduction （CTR） process. Firstly, V2O5, NH4H2PO4 and acetylene black are reacted under an Ar atmosphere to yield VPO4. The transition-metal reduction is facilitated by the CTR based on C→CO transition. These CTR conditions favor stabilization of the vanadium as V^3＋ as well as leaving residual carbon, which is useful in the subsequent electrode processing. Secondly, VPO4 reacts with ElF to yield LiVPO4F product. The property of the LiVPO4F was investigated by X-ray diffractometry （XRD）, scanning electron microscopy （SEM） and electrochemical measurement. XRD studies show that LiVPO4F synthesized has triclinic structure（space group p I ）, isostructural with the naturally occurring mineral tavorite, EiFePO4-OH. SEM image exhibits that the particle size is about 2μm together with homogenous distribution. Electrochemical test shows that the initial discharge capacity of LiVPO4F powder is 119 mA·h/g at the rate of 0.2C with an average discharge voltage of 4.2V （vs Ei/Li^＋）, and the capacity retains 89 mA·h/g after 30 cycles.
基金Projects(2021YFF0502100,2021YFB2600900)supported by the National Key R&D Program of ChinaProjects(52022085,52278461)supported by the National Natural Science Foundation of ChinaProject(22CXTD0051)supported by Sichuan Youth Science and Technology Innovation Team,China。
文摘The sunny-shady slopes effect is a phenomenon that impacts the temperature distribution of high-speed railway subgrades,resulting in uneven frost heaving deformation on the subgrade surface,which in turn causes static irregularity in the slab track.Based on the hydraulics theory,a thermal-hydro-mechanical(THM)coupled model of frozen soil is established and verified.We explore the process and characteristics of the temperature field and deformation of soil during the freezing process of high-speed railway subgrades and analyze the track irregularity variation law of China Railway Track SystemⅢslab tracks under uneven frost heaving deformation.The results show that,because the left and right slopes of high-speed railway subgrade are exposed to different amounts of solar radiation,which is the key factor causing uneven frost heaving of subgrade.Different strike angles cause changes in temperature of the subgrade’s upper part and the frost heaving amount on the surface,leading to differences in the deformation of the slab track structure:Increased strike angle weakens the rail level irregularity of the down line and marginally increases the rail level irregularity of the up line,and these become consistent in north-south directions.Therefore,when selecting railway lines in seasonal frozen areas,the west-east direction should be avoided to prevent the extremes in sunny-shady slopes effect on subgrades.
文摘Warming yang is one of the most important therapeutic methods for treating diseases. In Peaceful Holy Benevolent Prescriptions (太平圣惠方 a collection of more than ten thousand formulas) written by Dr. Wang Huaiyin (王怀隐) et al in the Song Dynasty in 992 AD, more than one hundred formulas for treating diabetes are all complied with the principles of supplementing qi and nourishing yin. Dr. Liu Hejian (刘河间) in the period of Jin and Yuan Dynasty advocated that it should be treated with the principles of clearing away pathogenic heat and fire, and supplementing qi and nourishing yin. Dr. Huang Kunzai (黄坤载) in the Qing Dynasty held that diabetes should be treated by supplementing qi and nourishing yin.
文摘Animal performance is affected by high air temperature and it is known that shade reduces the absorption of radiant temperature, and water for immersion facilitates heat loss. This study intends to find preferences of resources that contribute for the well-being of cattle and how they alterdaily behaviour. During summer, six Caracu and six Red Angus bulls were submitted to two different treatments： availability of artificial shade and water for immersion and availability of water for immersion. The categories observed were： positions （in the sun, under the shade, in the water）, posture （standing, lying down） and activities （grazing, ruminating leisure）. The behavioural patterns were recorded using the focal sampling method every 15 minutes （from 6：00 a.m. to 6：00 p.m.）. When shade and water for immersion coexists, cattle in this study prefer shade to avoid solar radiation. Both breeds had remained more time grazing, followed by ruminating in the Caracu breed, and by resting in the Red Angus breed. The Caracu breed had presented clear preference for the shade resource, but that fact was not always observed in the Red Angus breed. In hot climates, resources for defence against heat load, as shade and water for immersion improve the well-being of cattle.
文摘The heating area of the material is large and the thermal efficiency is high,but it is n ecessary to control the suitable drying temperature ofdiffere nt medici nal materials to preserve the effective in gredients.D iff ere nt kinds of Chinese medicine need different drying conditions to fulfill good drying requirements.Natural drying in the shade is one of the traditional drying methods,which takes a long time and is easily affected by the weather.The water volatilizes slowly.It is prone to mildew and discoloration during the drying process.However,it can better preserve the volatile oil components of Chinese medicine.The hot-air drying machine has lower requirements.The medicinal materials have a large heating area and high thermal efficiency,but it is necessary to control the appropriate drying temperature of different medicinal materials in order to preserve the active ingredients of the medicinal materials;it is not suitable for medicinal materials that stick and bind easily.The microwave drying method possesses superiority in drying some valuable medicinal materials such as Ren she n(Radix et Rhizoma Ginseng)and Lurong(Co「nu Cervi Pantotrichum),and the effective ingredients are preserved at a high degree;it can also achieve the purpose of killing enzymes and protecting glycosides and have a good bactericidal effect,but it is not suitable for Chinese medicines containing heat-sensitive ingredients,because it will destroy most of the proteins,amino acids,and peptides of Chinese medicine and result in the loss of efficacy.The far-infrared drying method is suitable for drying thin-layer medicinal materials and is friendly to the environment.Freeze-drying can preserve the active ingredients very well and greatly retain the efficacy,but it has obvious limitations in preserving some Chinese medicinal materials that need to kill enzymes and protect glycosides;besides,the cost is relatively high and the drying time is long.
