The lateral resistance of sleeper plays an important role in ensuring the stability of a railway track, which may change in the operation of railway, due to the fouling in the ballast bed. In this work, discrete eleme...The lateral resistance of sleeper plays an important role in ensuring the stability of a railway track, which may change in the operation of railway, due to the fouling in the ballast bed. In this work, discrete element method was adopted to investigate the effect of fouling on the lateral resistance of sleeper. The shape information of ballast was captured by method of three-dimensional vision reconstruction. In order to calibrate the mechanical parameters and verify the models, a lateral resistance field test was carried out by using a custom-made device. The contact force distributions in the different parts of sleeper as well as the interaction between ballast and sleeper were discussed in depth. The results show that fouling of ballast bed evidently reduces the lateral resistance of sleeper and the decreasing degree is also related to the fouled position of ballast bed, in the order of shoulder > bottom > side.Therefore, the effect of fouling, especially the fouling in the ballast shoulder, on the lateral resistance of sleeper, should be taken into account in ballast track maintenance work.展开更多
Despite advances in preoperative evaluation and postoperative care, intervention, especially surgery, for relief of obstructive jaundice still carries high morbidity and mortality rates, mainly due to sepsis and renal...Despite advances in preoperative evaluation and postoperative care, intervention, especially surgery, for relief of obstructive jaundice still carries high morbidity and mortality rates, mainly due to sepsis and renal dysfunction. The key event in the pathophysiology of obstructive jaundice-associated complications is endotoxemia of gut origin because of intestinal barrier failure. This breakage of the gut barrier in obstructive jaundice is multi-factorial, involving disruption of the immunologic, biological and mechanical barrier. Experimental and clinical studies have shown that obstructive jaundice results in increased intestinal permeability. The mechanisms implicated in this phenomenon remain unresolved, but growing research interest during the last decade has shed light in our knowledge in the field. This review summarizes the current concepts in the pathophysiology of obstructive jaundice-induced gut barrier dysfunction, analyzing pivotal factors, such as altered intestinal tight junctions expression, oxidative stress and imbalance of enterocyte proliferation and apoptosis. Clinicians handling patients with obstructive jaundice should not neglect protecting the intestinal barrier function before, during and after intervention for the relief of this condition, which may improve their patients’ outcome.展开更多
In situ testing provides accurate characterization of existing degradation mechanisms of materials and building systems which helps reducing the subjectivity of visual inspections especially when ageing under natural ...In situ testing provides accurate characterization of existing degradation mechanisms of materials and building systems which helps reducing the subjectivity of visual inspections especially when ageing under natural ageing conditions is carried out over significant periods of time. In this work, data collected from field experimental campaigns over 10 years after construction using several premixed one-coat rendered walls and over 4 and 6 years after construction in the case of ETICS （external thermal insulation composite systems） are presented and discussed. The influence of hydrophobic agents on the performance of both types of facades in terms of surface condition and water penetration was assessed by visual inspections, Karsten test and capillary tests. Additionally, adhesion tests and subsequent evaluation of porosity were also carried out for premixed one-coat mortars. The results obtained have shown that ageing of the rendering solutions, especially after 5 or 6 years in-service, results from the loss of resistance to liquid water penetration, especially at the surface. Yet, additional mechanisms such as leaching of hydrophobic agents and photochemical degradation are thought to be involved, too. Moreover, the results obtained using the Karsten test proved to be the most reliable method to determine the loss of water penetration resistance and predict the ageing of facade solutions. The information collected and the correlations established can help developing solutions to prevent early ageing and implement appropriate maintenance plans to delay their effect.展开更多
Objective： We investigated the influence of CDK1 and CDK2 expression inhibited by cotransfection of CDK1 and CDK2 siRNA on cell cycle and apoptosis, explored the exact role of cell cycle master regulator in tumor cel...Objective： We investigated the influence of CDK1 and CDK2 expression inhibited by cotransfection of CDK1 and CDK2 siRNA on cell cycle and apoptosis, explored the exact role of cell cycle master regulator in tumor cell apoptosis process. Methods： The siRNA targeting the CDK1 and CDK2 genes were synthesized and simultaneously cotransfected into Hela cells by lipofectamine 2000.48 or 60 h after the cotransfection, CDK1 and CDK2 protein expressions were examined by Western blot. Cell cycle distribution was analyzed by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The changes of the transfected cell morphological under a microscope after Wright-Giemsa Staining were studied. Results： CDK1 and CDK2 protein expression was decreased at 48 or 60 h after cotransfection. The accumulation of the G2/M and S phase population in cell cycle of the cotransfected cells at 48 or 60 h after transfection was enhanced obviously compared with control. The ratio of apoptotic cell of cotransfected cells at 48 or 60 h after transfection was increased significantly compared with control. More binucleate or multinucleate ceJls among cotransfected cells were observed under the microscope. Conclusion： The decreased expression of CDK1 and CDK2 by cotransfection of CDK1 and CDK2 siRNA not only leads to tumor cell cycle arrest in S phase and G2/M phase, but also induces tumor cell apoptosis.展开更多
A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield...A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield by using one rice variety Longliangyouhuazhan,and 4 kinds of remediation agents:DG foliar control agent,SMA microbial inoculant,XFJ organic fertilizer and LXM calcium-and silicon-based passivator as test materials.The results showed that compared with CK,after applying DG foliar control agent,soil available Cd content increased rather than decreased,whereas rice Cd content decreased,not reaching a significant level.When applied with SMA microbial inoculant,soil pH value,soil available Cd and rice Cd contents showed a downward then upward trend with the increase of its application rates.Between 3 levels of SMA treatments,the content of soil available Cd in SMA2 was the lowest,decreased by 8.59%in comparison with CK,contrarily,two other SMA treatments were increasing instead of decreasing in the content of soil available Cd.The application of XFJ organic fertilizer and LXM calcium-and silicon-based passivator increased soil pH value,and reduced soil available Cd and rice Cd contents;the contents of soil available Cd and rice Cd decreased with their application rates increasing.Compared with CK,XFJ3 reduced soil available Cd content by 9.40%,and significantly reduced rice Cd content by 57.28%.In LXM3 treatment,soil available Cd content reduced by 14.57%,rice Cd content was 71.57%lower than CK,reaching the lowest level.In general,LXM calcium-and silicon-based passivator had the best Cd reduction effect,with the optimal application amount of 6000 kg/hm^(2).展开更多
Enviromnental pollution is serious social concern. The inflow of heavy metals in the ecological food chain and their subsequent bio-magnification in human bodies is cascading its harmful effects. The metabolism of pla...Enviromnental pollution is serious social concern. The inflow of heavy metals in the ecological food chain and their subsequent bio-magnification in human bodies is cascading its harmful effects. The metabolism of plants is being hampered by these heavy metals. In the present studies, effect of heavy metal especially cadmium has been studied on stomatal diffusive resistance （SDR）, transpiration rate, leaf surface humidity and leaf temperature of soybean plants growing in hydroponic cultures. Cadmium treatment decelerates the rate of transpiration. The inhibition of transpiration associated with increased SDR leads to dehydration of leaf surface, thereby, increasing temperature of leaves in comparison to control plants. In the present study, temperature difference between stressed and non-stressed plant leaves was as high as 3℃ Moreover, any such rise in leaf temperature due to uptake and induced toxicity of heavy metals can possibly be a good bio-indicator having wide applications in thermal remote sensing and geospatial monitoring of metal polluted soils.展开更多
Air pollution is one of the leading cause of death for underdeveloped as well as developed countries. In 2011, the air pollution was the second leading cause of death in one of the main hospitals of the Kathmandu Vall...Air pollution is one of the leading cause of death for underdeveloped as well as developed countries. In 2011, the air pollution was the second leading cause of death in one of the main hospitals of the Kathmandu Valley, Nepal, and it was the third leading cause in the United States. Burning fossil fuels for transportation was one of the major causes. Among various impacts of the air pollution, COPD （chronic obstructive pulmonary disease） was one of the main consequences. In the 570 km2 Kathmandu Valley, vehicle registration was increased from 45,871 in 1990/1991 to 570,145 in 2010/2011, an increase of over 12-fold over 20 years. The vehicle registration and number of COPD patients＇ data were collected from various government divisions. In addition, the average daily travel distance and fuel mileage data were collected with a survey with residents of the Kathmandu Valley. This paper calculates the amount of carbon dioxide （CO2） emissions by transportation sector and determines correlation between CO2 emissions and COPD patients. Results show that there was a very high level of CO2 emissions, and it was positively correlated with the number of COPD patients in the valley.展开更多
基金Projects(U1234211,61472029,51208034)supported by the National Natural Science Foundation of China
文摘The lateral resistance of sleeper plays an important role in ensuring the stability of a railway track, which may change in the operation of railway, due to the fouling in the ballast bed. In this work, discrete element method was adopted to investigate the effect of fouling on the lateral resistance of sleeper. The shape information of ballast was captured by method of three-dimensional vision reconstruction. In order to calibrate the mechanical parameters and verify the models, a lateral resistance field test was carried out by using a custom-made device. The contact force distributions in the different parts of sleeper as well as the interaction between ballast and sleeper were discussed in depth. The results show that fouling of ballast bed evidently reduces the lateral resistance of sleeper and the decreasing degree is also related to the fouled position of ballast bed, in the order of shoulder > bottom > side.Therefore, the effect of fouling, especially the fouling in the ballast shoulder, on the lateral resistance of sleeper, should be taken into account in ballast track maintenance work.
文摘Despite advances in preoperative evaluation and postoperative care, intervention, especially surgery, for relief of obstructive jaundice still carries high morbidity and mortality rates, mainly due to sepsis and renal dysfunction. The key event in the pathophysiology of obstructive jaundice-associated complications is endotoxemia of gut origin because of intestinal barrier failure. This breakage of the gut barrier in obstructive jaundice is multi-factorial, involving disruption of the immunologic, biological and mechanical barrier. Experimental and clinical studies have shown that obstructive jaundice results in increased intestinal permeability. The mechanisms implicated in this phenomenon remain unresolved, but growing research interest during the last decade has shed light in our knowledge in the field. This review summarizes the current concepts in the pathophysiology of obstructive jaundice-induced gut barrier dysfunction, analyzing pivotal factors, such as altered intestinal tight junctions expression, oxidative stress and imbalance of enterocyte proliferation and apoptosis. Clinicians handling patients with obstructive jaundice should not neglect protecting the intestinal barrier function before, during and after intervention for the relief of this condition, which may improve their patients’ outcome.
文摘In situ testing provides accurate characterization of existing degradation mechanisms of materials and building systems which helps reducing the subjectivity of visual inspections especially when ageing under natural ageing conditions is carried out over significant periods of time. In this work, data collected from field experimental campaigns over 10 years after construction using several premixed one-coat rendered walls and over 4 and 6 years after construction in the case of ETICS （external thermal insulation composite systems） are presented and discussed. The influence of hydrophobic agents on the performance of both types of facades in terms of surface condition and water penetration was assessed by visual inspections, Karsten test and capillary tests. Additionally, adhesion tests and subsequent evaluation of porosity were also carried out for premixed one-coat mortars. The results obtained have shown that ageing of the rendering solutions, especially after 5 or 6 years in-service, results from the loss of resistance to liquid water penetration, especially at the surface. Yet, additional mechanisms such as leaching of hydrophobic agents and photochemical degradation are thought to be involved, too. Moreover, the results obtained using the Karsten test proved to be the most reliable method to determine the loss of water penetration resistance and predict the ageing of facade solutions. The information collected and the correlations established can help developing solutions to prevent early ageing and implement appropriate maintenance plans to delay their effect.
基金Supported by grants from the National Basic Research Program of China (No. 2004CB518705)National Natural Sciences Foundation of China (No.30570908)
文摘Objective： We investigated the influence of CDK1 and CDK2 expression inhibited by cotransfection of CDK1 and CDK2 siRNA on cell cycle and apoptosis, explored the exact role of cell cycle master regulator in tumor cell apoptosis process. Methods： The siRNA targeting the CDK1 and CDK2 genes were synthesized and simultaneously cotransfected into Hela cells by lipofectamine 2000.48 or 60 h after the cotransfection, CDK1 and CDK2 protein expressions were examined by Western blot. Cell cycle distribution was analyzed by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The changes of the transfected cell morphological under a microscope after Wright-Giemsa Staining were studied. Results： CDK1 and CDK2 protein expression was decreased at 48 or 60 h after cotransfection. The accumulation of the G2/M and S phase population in cell cycle of the cotransfected cells at 48 or 60 h after transfection was enhanced obviously compared with control. The ratio of apoptotic cell of cotransfected cells at 48 or 60 h after transfection was increased significantly compared with control. More binucleate or multinucleate ceJls among cotransfected cells were observed under the microscope. Conclusion： The decreased expression of CDK1 and CDK2 by cotransfection of CDK1 and CDK2 siRNA not only leads to tumor cell cycle arrest in S phase and G2/M phase, but also induces tumor cell apoptosis.
文摘A field experiment was carried out in Cd-contaminated rice fields in a county of Hengyang to explore the effects of different types of remediation agents on the contents of soil available Cd and rice Cd,and rice yield by using one rice variety Longliangyouhuazhan,and 4 kinds of remediation agents:DG foliar control agent,SMA microbial inoculant,XFJ organic fertilizer and LXM calcium-and silicon-based passivator as test materials.The results showed that compared with CK,after applying DG foliar control agent,soil available Cd content increased rather than decreased,whereas rice Cd content decreased,not reaching a significant level.When applied with SMA microbial inoculant,soil pH value,soil available Cd and rice Cd contents showed a downward then upward trend with the increase of its application rates.Between 3 levels of SMA treatments,the content of soil available Cd in SMA2 was the lowest,decreased by 8.59%in comparison with CK,contrarily,two other SMA treatments were increasing instead of decreasing in the content of soil available Cd.The application of XFJ organic fertilizer and LXM calcium-and silicon-based passivator increased soil pH value,and reduced soil available Cd and rice Cd contents;the contents of soil available Cd and rice Cd decreased with their application rates increasing.Compared with CK,XFJ3 reduced soil available Cd content by 9.40%,and significantly reduced rice Cd content by 57.28%.In LXM3 treatment,soil available Cd content reduced by 14.57%,rice Cd content was 71.57%lower than CK,reaching the lowest level.In general,LXM calcium-and silicon-based passivator had the best Cd reduction effect,with the optimal application amount of 6000 kg/hm^(2).
文摘Enviromnental pollution is serious social concern. The inflow of heavy metals in the ecological food chain and their subsequent bio-magnification in human bodies is cascading its harmful effects. The metabolism of plants is being hampered by these heavy metals. In the present studies, effect of heavy metal especially cadmium has been studied on stomatal diffusive resistance （SDR）, transpiration rate, leaf surface humidity and leaf temperature of soybean plants growing in hydroponic cultures. Cadmium treatment decelerates the rate of transpiration. The inhibition of transpiration associated with increased SDR leads to dehydration of leaf surface, thereby, increasing temperature of leaves in comparison to control plants. In the present study, temperature difference between stressed and non-stressed plant leaves was as high as 3℃ Moreover, any such rise in leaf temperature due to uptake and induced toxicity of heavy metals can possibly be a good bio-indicator having wide applications in thermal remote sensing and geospatial monitoring of metal polluted soils.
文摘Air pollution is one of the leading cause of death for underdeveloped as well as developed countries. In 2011, the air pollution was the second leading cause of death in one of the main hospitals of the Kathmandu Valley, Nepal, and it was the third leading cause in the United States. Burning fossil fuels for transportation was one of the major causes. Among various impacts of the air pollution, COPD （chronic obstructive pulmonary disease） was one of the main consequences. In the 570 km2 Kathmandu Valley, vehicle registration was increased from 45,871 in 1990/1991 to 570,145 in 2010/2011, an increase of over 12-fold over 20 years. The vehicle registration and number of COPD patients＇ data were collected from various government divisions. In addition, the average daily travel distance and fuel mileage data were collected with a survey with residents of the Kathmandu Valley. This paper calculates the amount of carbon dioxide （CO2） emissions by transportation sector and determines correlation between CO2 emissions and COPD patients. Results show that there was a very high level of CO2 emissions, and it was positively correlated with the number of COPD patients in the valley.
