目的探讨阿尔茨海默病性痴呆(Alzheimer’s disease,AD)患者血清铜、铁、锌与其痴呆程度的关系。方法纳入125例AD患者及40例健康对照者,AD患者根据简明心理状况测验(mini-mental state examination,MMSE)评分分为轻度痴呆组(21~26分)、...目的探讨阿尔茨海默病性痴呆(Alzheimer’s disease,AD)患者血清铜、铁、锌与其痴呆程度的关系。方法纳入125例AD患者及40例健康对照者,AD患者根据简明心理状况测验(mini-mental state examination,MMSE)评分分为轻度痴呆组(21~26分)、中度痴呆组(10~20分)及重度痴呆组(<10分),所有受试者均测定血清铜、铁、锌含量。结果与对照组及轻度痴呆组比较,中度和重度痴呆组血清铜含量的差异具有统计学意义,提示与对照组及轻度痴呆组比较,中度和重度痴呆组患者体内血清铜含量增加。与对照组比较,轻度、中度和重度痴呆组血清铁含量的差异具有统计学意义,提示与对照组比较,AD患者体内血清铁含量减少。与对照组比较,轻度、中度和重度痴呆组血清锌含量的差异具有统计学意义,提示与对照组比较,AD患者体内血清锌含量减少。相关分析提示AD患者MMSE评分与血清铜含量呈负相关,与血清锌含量呈正相关,而与血清铁含量无相关性,说明痴呆程度越重,血清铜含量越高,血清锌含量越低。结论 AD患者体内存在着微量元素的稳态失衡,AD患者血清中有些微量元素如铜、锌等的水平与AD的痴呆程度有一定的相关性。展开更多
Senile Dementia is the illness with a symptom of ongoing cognitive obstacle and loss of memory function. With our population aging, dementia and depression in old age is increasing rapidly. It is estimated that by 202...Senile Dementia is the illness with a symptom of ongoing cognitive obstacle and loss of memory function. With our population aging, dementia and depression in old age is increasing rapidly. It is estimated that by 2020, depressive disorder will become the second largest human disease leading to crippling. By 2040, globally the number of people with dementia will reach 81.1 million while the number of dementia patients in China will be the sum of that in all developed countries. Its incidence increases exponentially with age and the incidence of the elderly over 85 reach up to 8% -10%. Among all dementia patients, people with Alzheimer＇ s disease （Alzheimer＇ s disease, AD） accounted for 50 % -70%, the rest is vascular dementia （vascular dementia, VD） and mixed dementia. In the United States, Alzheimer＇ s disease has become the fourth leading cause of death followed after cardiovascular disease, cancer and stroke. Through comprehensive control strategy, we can improve the mental health level of old people, so as to protect the physical and mental health, improving the life quality of old people.展开更多
Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments...Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.展开更多
文摘目的探讨阿尔茨海默病性痴呆(Alzheimer’s disease,AD)患者血清铜、铁、锌与其痴呆程度的关系。方法纳入125例AD患者及40例健康对照者,AD患者根据简明心理状况测验(mini-mental state examination,MMSE)评分分为轻度痴呆组(21~26分)、中度痴呆组(10~20分)及重度痴呆组(<10分),所有受试者均测定血清铜、铁、锌含量。结果与对照组及轻度痴呆组比较,中度和重度痴呆组血清铜含量的差异具有统计学意义,提示与对照组及轻度痴呆组比较,中度和重度痴呆组患者体内血清铜含量增加。与对照组比较,轻度、中度和重度痴呆组血清铁含量的差异具有统计学意义,提示与对照组比较,AD患者体内血清铁含量减少。与对照组比较,轻度、中度和重度痴呆组血清锌含量的差异具有统计学意义,提示与对照组比较,AD患者体内血清锌含量减少。相关分析提示AD患者MMSE评分与血清铜含量呈负相关,与血清锌含量呈正相关,而与血清铁含量无相关性,说明痴呆程度越重,血清铜含量越高,血清锌含量越低。结论 AD患者体内存在着微量元素的稳态失衡,AD患者血清中有些微量元素如铜、锌等的水平与AD的痴呆程度有一定的相关性。
文摘Senile Dementia is the illness with a symptom of ongoing cognitive obstacle and loss of memory function. With our population aging, dementia and depression in old age is increasing rapidly. It is estimated that by 2020, depressive disorder will become the second largest human disease leading to crippling. By 2040, globally the number of people with dementia will reach 81.1 million while the number of dementia patients in China will be the sum of that in all developed countries. Its incidence increases exponentially with age and the incidence of the elderly over 85 reach up to 8% -10%. Among all dementia patients, people with Alzheimer＇ s disease （Alzheimer＇ s disease, AD） accounted for 50 % -70%, the rest is vascular dementia （vascular dementia, VD） and mixed dementia. In the United States, Alzheimer＇ s disease has become the fourth leading cause of death followed after cardiovascular disease, cancer and stroke. Through comprehensive control strategy, we can improve the mental health level of old people, so as to protect the physical and mental health, improving the life quality of old people.
文摘Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.
