大都会歌剧院这两年的营销主题是"必须聆听的声音"(The voice must be heard),这个口号有着多重意义,比如表示人声是歌剧的精髓,或是要听到没有加工的原声得进剧院,以及人都会有着顶尖的声乐演唱家不容错过,等等。大都会一直都是一...大都会歌剧院这两年的营销主题是"必须聆听的声音"(The voice must be heard),这个口号有着多重意义,比如表示人声是歌剧的精髓,或是要听到没有加工的原声得进剧院,以及人都会有着顶尖的声乐演唱家不容错过,等等。大都会一直都是一个以歌手为重的歌剧院,明星歌手层出不穷,本季开场的《诺尔玛》(Norma)就是证明。这个公认极为难唱的角色,大都会本季一共排出三位女高音,展开更多
Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments...Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.展开更多
首席执行官阿达尔·波纳瓦拉(Adar Poonawalla)执掌的印度血清研究所(Serum Institute of India)是全球最大的疫苗供应商,每年能生产约15亿剂人体接种疫苗,几乎涵盖各个门类。该公司拥有的设备每分钟能灌装500支小玻璃瓶,数座闪闪...首席执行官阿达尔·波纳瓦拉(Adar Poonawalla)执掌的印度血清研究所(Serum Institute of India)是全球最大的疫苗供应商,每年能生产约15亿剂人体接种疫苗,几乎涵盖各个门类。该公司拥有的设备每分钟能灌装500支小玻璃瓶,数座闪闪发光、接近两层楼高的钢质生物反应器每个月能生成超过1000万剂疫苗的药液。他可以令人信服地宣称,他为分布在100多个国家和地区的全世界65%的儿童预防麻疹和肺结核等疾病做出了贡献。展开更多
文摘大都会歌剧院这两年的营销主题是"必须聆听的声音"(The voice must be heard),这个口号有着多重意义,比如表示人声是歌剧的精髓,或是要听到没有加工的原声得进剧院,以及人都会有着顶尖的声乐演唱家不容错过,等等。大都会一直都是一个以歌手为重的歌剧院,明星歌手层出不穷,本季开场的《诺尔玛》(Norma)就是证明。这个公认极为难唱的角色,大都会本季一共排出三位女高音,
文摘Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.
文摘首席执行官阿达尔·波纳瓦拉(Adar Poonawalla)执掌的印度血清研究所(Serum Institute of India)是全球最大的疫苗供应商,每年能生产约15亿剂人体接种疫苗,几乎涵盖各个门类。该公司拥有的设备每分钟能灌装500支小玻璃瓶,数座闪闪发光、接近两层楼高的钢质生物反应器每个月能生成超过1000万剂疫苗的药液。他可以令人信服地宣称,他为分布在100多个国家和地区的全世界65%的儿童预防麻疹和肺结核等疾病做出了贡献。