Objective] This study aimed to determine the effects of medium composi-tion and fermentation condition on the production of antibiotics from an actinomycete antagonistic to Penicil ium viridicatum, which was isolated ...Objective] This study aimed to determine the effects of medium composi-tion and fermentation condition on the production of antibiotics from an actinomycete antagonistic to Penicil ium viridicatum, which was isolated from soil and identified primarily as Streptomyces anandi . [Method] Single-factor experiments and orthogonal array designs were used to optimize the medium composition and fermentation con-ditions. [Result] Culture medium composition and fermentation conditions showed sig-nificant influence on the antibiotic production from S. anandi . The optimum culture medium consisted of soluble starch 10 g/L, soybean cake powder 15 g/L, KH2PO4 0.5 g/L and MgSO4·7H2O 0.1 g/L. The optimized fermentation conditions were tem-perature 30 ℃, initial pH value 7.7, culture medium amount 40 ml/250 ml flask, and fermentation time 144 h. The titer of fermentation broth reached 452.7 U/ml when the S. anandi was cultured under the optimal fermentation medium and conditions. [Conclusion] The study may lay the foundation on further isolation and purification of the antibiotic from S. anandi JXNU02.展开更多
The aim of this study was to investigate the body weight models among adolescent school students and to establish the national prevalence of being overweight and obesity in Albanian adolescents. Overweight and obesity...The aim of this study was to investigate the body weight models among adolescent school students and to establish the national prevalence of being overweight and obesity in Albanian adolescents. Overweight and obesity is a global public health concern. There is limited information on overweight and obesity in Albanian adolescents. A cross-sectional study on a stratified sample of 1,786 adolescents who attended school, 705 （39%） males and 1,081 （61%） females was conducted in Albania in January-December 2011. They were interviewed and examined for weight and height using standardized techniques. Referring to the 2007 WHO classification for the BMI scale it resulted that 4.4% 95% CI 3.5 - 5.4 adolescents resulted overweight where 32.9% were females and 67.1% were males with a statistically significant difference among them, P 〈 0.01. The overall prevalence of overweight in adolescent females was 2.4%, while in males was 7.5%. The prevalence of obesity class I resulted 0.4%, the obesity class II was 0.3% and obesity class III was 0.1% 95% CI 0.02 - 0.3 from the total of participants. A remarkable finding of our study was that 70.8% of adolescent school students were in normal weight, 20% of them underweight, where 71.7% were females, P 〈 0.01.展开更多
The results of studies of radiation chemical transformations of Balakhani bituminous oil of Azerbaijan are adduced. The IR （infrared） spectra of initial and irradiated samples of tar fractions of bituminous oil are ...The results of studies of radiation chemical transformations of Balakhani bituminous oil of Azerbaijan are adduced. The IR （infrared） spectra of initial and irradiated samples of tar fractions of bituminous oil are compared. The kinetics of radiation chemical yields of gas products at the irradiation of bituminous oil and its tar fraction have been investigated. Irradiation is conducted in the gamma-ray source of isotope 60Co at the dose rate of P = 0.27 Gy/s and absorbed doses D = 5-163 kGy. It is found that in comparison with oil faction, tar fraction of bituminous oil has a high resistance to radiation.展开更多
The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fis...The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fishmeal maintain same growth rate in semi intensive system, compared to diets containing 8% and 20% fish meal. Feeding trials were carried out in CENADAC （27°32′ S, 58030＇ W） in a semi intensive pond culture system for 122 days. Two experimental feed including fish silage, a liquid product obtained of fish residues treated by enzymes action, were formulated. Fish silage was prepared by mincing and blending fish viscera with sufficient formic acid （2.11% v/w） to attain a stable pH of 3.5. Fish were daily fed initially with the dry ration at 1.3% bw/d and at a rate of 0.55 g of protein/100 g of fish with the wet ration. At the end of the experience the average weight observed for dry fed fish was 1,513.5 g and 1,560.3 g for those wet fed. The daily growth was 3.69 g/d for the first group and 3.9 g/d for the second one. The final FCR was 2.72 and 3.01 for each group without significant differences. These results showed that it is possible to grow out paco with wet self made feed without fish meal and with similar growth that those fed dry diet.展开更多
OBJECTIVE:To investigate the effects of artemisinin against proteinuria and glomerular filtration barrier damage in rats with adriamycin-induced nephropathy,and the potential mechanism underpinned the action.METHODS:F...OBJECTIVE:To investigate the effects of artemisinin against proteinuria and glomerular filtration barrier damage in rats with adriamycin-induced nephropathy,and the potential mechanism underpinned the action.METHODS:Forty adriamycin rats were randomly divided into two groups with the ratio of 1︰3;the small-number group served as control group(n=10),and the rats in the large-number group were treated with adriamycin to induce nephropathy;then they were further randomly assigned into 3subgroups:benazepril group(n=10),artemisiningroup(n=10),and adriamycin group(n=10).The benazepril group and artemisinin group were treated with benazepril suspl(5.0 mg/kg daily)and artemisinin suspl(150 mg/kg daily)respectively after being modeled;those in the control group and adriamycin group were intragastrically administered an equivalent volume of distilled water every day.The treatment after model establishment lasted for a total of 4 weeks.The 24 h uric protein,blood biochemicals,renal pathological changes,renal ultrastrutural changes,Nephrin and Podocin proteins and gene expressions were measured by Coomassie brilliant blue assay,completely automatic biochemical analyzer,light microscope,electron microscopy,Western blot and reverse transcription polymerase chain reaction,respectively.RESULTS:The rats in adriamycin group showed a significant increase in 24 h uric protein excretion,serum total cholesterol(TC),triglyceride(TG),blood urea nitrogen(BUN),serum creatinine(Scr)and decrease in albumin(Alb)(P<0.05 or P<0.01).Compared with adriamycin group,artemisinin could reduce uric protein excretion,decrease the serum TC,TG elevation,increase the serum Alb level,up-regulate the expressions of Nephrin and Podocin(P<0.05 or P<0.01),but no statistical significance effects on the levels of BUN,Scr in artemisinin group(P>0.05).The renal pathological and ultrastrutural observation indicate that artemisinin could attenuate the severity of foot process effacement and fusion in the nephropathic rats.CONCLUSION:Artemisinin might have an effect on the nephropathy in rats caused by adriamycin,which may be at least partly correlated with attenu-ation of the severity of foot process effacement and fusion,up-regulation of the expressions of Nephrin and Podocin in the glomeruli in the rats.展开更多
基金Supported by the National Natural Science Foundation of China(31160029,31360018)the Natural Science Foundation of Jiangxi Province(20122BAB204008,20132BAB204007)+1 种基金Science and Technology Planning Project from Department of Education of Jiangxi Province(GJJ12181)Special Fund for Agro-scientific Research in the Public Interest(201203072)~~
文摘Objective] This study aimed to determine the effects of medium composi-tion and fermentation condition on the production of antibiotics from an actinomycete antagonistic to Penicil ium viridicatum, which was isolated from soil and identified primarily as Streptomyces anandi . [Method] Single-factor experiments and orthogonal array designs were used to optimize the medium composition and fermentation con-ditions. [Result] Culture medium composition and fermentation conditions showed sig-nificant influence on the antibiotic production from S. anandi . The optimum culture medium consisted of soluble starch 10 g/L, soybean cake powder 15 g/L, KH2PO4 0.5 g/L and MgSO4·7H2O 0.1 g/L. The optimized fermentation conditions were tem-perature 30 ℃, initial pH value 7.7, culture medium amount 40 ml/250 ml flask, and fermentation time 144 h. The titer of fermentation broth reached 452.7 U/ml when the S. anandi was cultured under the optimal fermentation medium and conditions. [Conclusion] The study may lay the foundation on further isolation and purification of the antibiotic from S. anandi JXNU02.
文摘The aim of this study was to investigate the body weight models among adolescent school students and to establish the national prevalence of being overweight and obesity in Albanian adolescents. Overweight and obesity is a global public health concern. There is limited information on overweight and obesity in Albanian adolescents. A cross-sectional study on a stratified sample of 1,786 adolescents who attended school, 705 （39%） males and 1,081 （61%） females was conducted in Albania in January-December 2011. They were interviewed and examined for weight and height using standardized techniques. Referring to the 2007 WHO classification for the BMI scale it resulted that 4.4% 95% CI 3.5 - 5.4 adolescents resulted overweight where 32.9% were females and 67.1% were males with a statistically significant difference among them, P 〈 0.01. The overall prevalence of overweight in adolescent females was 2.4%, while in males was 7.5%. The prevalence of obesity class I resulted 0.4%, the obesity class II was 0.3% and obesity class III was 0.1% 95% CI 0.02 - 0.3 from the total of participants. A remarkable finding of our study was that 70.8% of adolescent school students were in normal weight, 20% of them underweight, where 71.7% were females, P 〈 0.01.
文摘The results of studies of radiation chemical transformations of Balakhani bituminous oil of Azerbaijan are adduced. The IR （infrared） spectra of initial and irradiated samples of tar fractions of bituminous oil are compared. The kinetics of radiation chemical yields of gas products at the irradiation of bituminous oil and its tar fraction have been investigated. Irradiation is conducted in the gamma-ray source of isotope 60Co at the dose rate of P = 0.27 Gy/s and absorbed doses D = 5-163 kGy. It is found that in comparison with oil faction, tar fraction of bituminous oil has a high resistance to radiation.
文摘The effect of two diets containing fish acid silage were studied, the first one offered as dry diet and the second one as a wet diet, in final culture cycle of PactS. Previous experiences showed that diets without fishmeal maintain same growth rate in semi intensive system, compared to diets containing 8% and 20% fish meal. Feeding trials were carried out in CENADAC （27°32′ S, 58030＇ W） in a semi intensive pond culture system for 122 days. Two experimental feed including fish silage, a liquid product obtained of fish residues treated by enzymes action, were formulated. Fish silage was prepared by mincing and blending fish viscera with sufficient formic acid （2.11% v/w） to attain a stable pH of 3.5. Fish were daily fed initially with the dry ration at 1.3% bw/d and at a rate of 0.55 g of protein/100 g of fish with the wet ration. At the end of the experience the average weight observed for dry fed fish was 1,513.5 g and 1,560.3 g for those wet fed. The daily growth was 3.69 g/d for the first group and 3.9 g/d for the second one. The final FCR was 2.72 and 3.01 for each group without significant differences. These results showed that it is possible to grow out paco with wet self made feed without fish meal and with similar growth that those fed dry diet.
基金Supported by the National Natural Science Foundation of China(No.30801504,30901926,81100530,81070590)The Sci-tech Project of Shaanxi Province(No.2012K19-04-01)
文摘OBJECTIVE:To investigate the effects of artemisinin against proteinuria and glomerular filtration barrier damage in rats with adriamycin-induced nephropathy,and the potential mechanism underpinned the action.METHODS:Forty adriamycin rats were randomly divided into two groups with the ratio of 1︰3;the small-number group served as control group(n=10),and the rats in the large-number group were treated with adriamycin to induce nephropathy;then they were further randomly assigned into 3subgroups:benazepril group(n=10),artemisiningroup(n=10),and adriamycin group(n=10).The benazepril group and artemisinin group were treated with benazepril suspl(5.0 mg/kg daily)and artemisinin suspl(150 mg/kg daily)respectively after being modeled;those in the control group and adriamycin group were intragastrically administered an equivalent volume of distilled water every day.The treatment after model establishment lasted for a total of 4 weeks.The 24 h uric protein,blood biochemicals,renal pathological changes,renal ultrastrutural changes,Nephrin and Podocin proteins and gene expressions were measured by Coomassie brilliant blue assay,completely automatic biochemical analyzer,light microscope,electron microscopy,Western blot and reverse transcription polymerase chain reaction,respectively.RESULTS:The rats in adriamycin group showed a significant increase in 24 h uric protein excretion,serum total cholesterol(TC),triglyceride(TG),blood urea nitrogen(BUN),serum creatinine(Scr)and decrease in albumin(Alb)(P<0.05 or P<0.01).Compared with adriamycin group,artemisinin could reduce uric protein excretion,decrease the serum TC,TG elevation,increase the serum Alb level,up-regulate the expressions of Nephrin and Podocin(P<0.05 or P<0.01),but no statistical significance effects on the levels of BUN,Scr in artemisinin group(P>0.05).The renal pathological and ultrastrutural observation indicate that artemisinin could attenuate the severity of foot process effacement and fusion in the nephropathic rats.CONCLUSION:Artemisinin might have an effect on the nephropathy in rats caused by adriamycin,which may be at least partly correlated with attenu-ation of the severity of foot process effacement and fusion,up-regulation of the expressions of Nephrin and Podocin in the glomeruli in the rats.
