目的建立乌头附子类HPCE-DAD指纹图谱分析方法,并对乌头、附子及炮制品的指纹谱进行比较。方法以40mmol/L乙酸钠-60mmol/L乙酸铵-80 mmol/L tris试剂-25%甲醇(p H 8.3)为运行缓冲液,分离电压为16 k V,检测波长为235nm,以苯甲酰次乌头原...目的建立乌头附子类HPCE-DAD指纹图谱分析方法,并对乌头、附子及炮制品的指纹谱进行比较。方法以40mmol/L乙酸钠-60mmol/L乙酸铵-80 mmol/L tris试剂-25%甲醇(p H 8.3)为运行缓冲液,分离电压为16 k V,检测波长为235nm,以苯甲酰次乌头原碱为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果初步建立了以7个共有峰为特征指纹信息的乌头附子类HPCE-DAD指纹图谱;发现生品与炮制品的HPCE-DAD指纹谱有显著差异。结论方法准确可靠,重现性好,可作为乌头附子类药材内在质量评价的依据。展开更多
A single adsorption isothermal study was performed over HY and BEA zeolites in order to determine their adsorption capacities for phenol, ortho-nitrophenol and para-nitrophenol. The experiments were realized in batch ...A single adsorption isothermal study was performed over HY and BEA zeolites in order to determine their adsorption capacities for phenol, ortho-nitrophenol and para-nitrophenol. The experiments were realized in batch reactor and the isotherms were modelized by the Fowler-Guggenheim equation. During the adsorption process weak zeolite-sorbate interactions and more significant sorbate-sorbate attractions were identified. The adsorption was not linked to the molecular size of the sorbates and a strong correlation was established between the adsorption compound was the best adsorbed. The removal performances capacities and the dipole moments of the sorbates. The most polar of the zeolites depended on their hydrophobicity.展开更多
Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectr...Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum. The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%, This result indicates the tryptophan residue is essential for the interaction between ssDNA and IgGRF .and ionic-bonding plays an important role in adsorption.展开更多
Objective： To observe the effects of moxibustion therapy at Shenshu （BL 23） and Zusanli （ST 36） of rats with induced rheumatoid arthritis （RA） on the serumal intercellular cell adhesion molecule-1 （ICMA-1） an...Objective： To observe the effects of moxibustion therapy at Shenshu （BL 23） and Zusanli （ST 36） of rats with induced rheumatoid arthritis （RA） on the serumal intercellular cell adhesion molecule-1 （ICMA-1） and the pathological tissue, to discuss the mechanism of the warming and activating effect of moxibustion. Methods： After establishing the RA rats model, the induced rats were treated with moxibustion therapy on the acupoint Shenshu （BL 23） and Zusanli （ST 36）, followed by analyzing the pathological section of the ankle of the hind limb and testing the ICAM-1 content with ELISA. Results： The plantar circumferences of the induced rat increased significantly compared with the rats in the control group （P〈0.01）, accompanying with the increase of the synovial layer, the erosion of phlogocytes to chondrocytes and the specific increase of ICAM- 1 content. After the moxibustion therapy, the plantar circumferences decreased significantly （P〈0.01） while the synovial layer tended to reduce. In addition, there was no pathological damage of the articular cartilage and the ICAM content decreased with significant deviation （P〈0.01）, compared to the model group. Conclusion： It was concluded that moxibustion therapy could inhibit the arthrosynovitis and hyperplasia, ameliorate the erosion of phlogocyte to cartilage, prevent articular periosteal lesions and delay the pathological course. The warming and activating effect of moxibustion therapy may involve the inhibition of the formation of ICAM- 1 and pannus.展开更多
文摘目的建立乌头附子类HPCE-DAD指纹图谱分析方法,并对乌头、附子及炮制品的指纹谱进行比较。方法以40mmol/L乙酸钠-60mmol/L乙酸铵-80 mmol/L tris试剂-25%甲醇(p H 8.3)为运行缓冲液,分离电压为16 k V,检测波长为235nm,以苯甲酰次乌头原碱为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果初步建立了以7个共有峰为特征指纹信息的乌头附子类HPCE-DAD指纹图谱;发现生品与炮制品的HPCE-DAD指纹谱有显著差异。结论方法准确可靠,重现性好,可作为乌头附子类药材内在质量评价的依据。
文摘A single adsorption isothermal study was performed over HY and BEA zeolites in order to determine their adsorption capacities for phenol, ortho-nitrophenol and para-nitrophenol. The experiments were realized in batch reactor and the isotherms were modelized by the Fowler-Guggenheim equation. During the adsorption process weak zeolite-sorbate interactions and more significant sorbate-sorbate attractions were identified. The adsorption was not linked to the molecular size of the sorbates and a strong correlation was established between the adsorption compound was the best adsorbed. The removal performances capacities and the dipole moments of the sorbates. The most polar of the zeolites depended on their hydrophobicity.
基金National Basic Science and Development of China (G1999O64707)
文摘Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum. The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%, This result indicates the tryptophan residue is essential for the interaction between ssDNA and IgGRF .and ionic-bonding plays an important role in adsorption.
文摘Objective： To observe the effects of moxibustion therapy at Shenshu （BL 23） and Zusanli （ST 36） of rats with induced rheumatoid arthritis （RA） on the serumal intercellular cell adhesion molecule-1 （ICMA-1） and the pathological tissue, to discuss the mechanism of the warming and activating effect of moxibustion. Methods： After establishing the RA rats model, the induced rats were treated with moxibustion therapy on the acupoint Shenshu （BL 23） and Zusanli （ST 36）, followed by analyzing the pathological section of the ankle of the hind limb and testing the ICAM-1 content with ELISA. Results： The plantar circumferences of the induced rat increased significantly compared with the rats in the control group （P〈0.01）, accompanying with the increase of the synovial layer, the erosion of phlogocytes to chondrocytes and the specific increase of ICAM- 1 content. After the moxibustion therapy, the plantar circumferences decreased significantly （P〈0.01） while the synovial layer tended to reduce. In addition, there was no pathological damage of the articular cartilage and the ICAM content decreased with significant deviation （P〈0.01）, compared to the model group. Conclusion： It was concluded that moxibustion therapy could inhibit the arthrosynovitis and hyperplasia, ameliorate the erosion of phlogocyte to cartilage, prevent articular periosteal lesions and delay the pathological course. The warming and activating effect of moxibustion therapy may involve the inhibition of the formation of ICAM- 1 and pannus.
