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磁湖初期雨水污染影响下的附生藻群落结构
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作者 李今 吴真真 +3 位作者 华江环 吉芬芬 张勇 吕爱斌 《湖北师范大学学报(自然科学版)》 2017年第4期11-14,共4页
对黄石市磁湖地表雨水径流污染进行了调查,研究了受纳水体中沉水植物菹草表面的附生藻的群落结构特征。结果显示,磁湖地表雨水径流中氨氮(NH_3-N)和总磷(TP)的浓度远高于雨水;生活区地表雨水径流中氨氮(NH_3-N)和化学需氧量(COD)的浓度... 对黄石市磁湖地表雨水径流污染进行了调查,研究了受纳水体中沉水植物菹草表面的附生藻的群落结构特征。结果显示,磁湖地表雨水径流中氨氮(NH_3-N)和总磷(TP)的浓度远高于雨水;生活区地表雨水径流中氨氮(NH_3-N)和化学需氧量(COD)的浓度大于绿地区域。与此同时,菹草表面附生藻共有60种,隶属于7门,34属。附生藻的平均生物量、附生密度分别为1.28×10~2 mg/cm^2、12.12×10~3cells/cm^2。藻类Shannon-Wiener多样性指数值在2~3之间。 展开更多
关键词 雨水径流 附生藻 群落结构 水环境
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秦皇岛海域秋季石花菜(Gelidium amansii)附生大型海藻的实验分类学研究 被引量:1
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作者 张瑶 闫璟 +5 位作者 黄冰心 郭优璇 梁俊霞 王雪聪 闫盼竹 丁兰平 《海洋与湖沼》 CAS CSCD 北大核心 2023年第2期493-501,共9页
秦皇岛海域属于我国渤海区域典型的大型海藻分布区,该海区资源丰富,在我国大型海藻区系中占有较为重要的位置。于2021年秋季在该海域采集石花菜(Gelidium amansii)样品进行活体培养,对其藻体附生的其他季节大型海藻的状况加以观察研究... 秦皇岛海域属于我国渤海区域典型的大型海藻分布区,该海区资源丰富,在我国大型海藻区系中占有较为重要的位置。于2021年秋季在该海域采集石花菜(Gelidium amansii)样品进行活体培养,对其藻体附生的其他季节大型海藻的状况加以观察研究。通过实验生物学方法对石花菜附生藻生殖特征和生态习性进行了观察,结合附生藻形态特征进行物种分类鉴定。经过培养与鉴定,确定该水域石花菜的附生大型海藻共有7种,其中红藻4种,即丛出旋体藻(Audouinelladaviesii)、旋体藻(Auduinella sp.)、茎丝藻小枝变型(Stylonema alsidii f. ramosum)和日本新管藻(Neosiphonia japonica);绿藻2种,即苍白刚毛藻(Cladophora albida)和浒苔[Enteromorpha (Ulva) prolifera];蓝藻1种,即丝状鞘丝藻(Lyngbya confervoides);丛出旋体藻、茎丝藻小枝变型和苍白刚毛藻为河北海域的新记录物种。研究结果表明,室内人工培养可作为个体微小的附生大型海藻分类鉴定的有效补充方法,也能为分子鉴定提供纯净的样品来源,同时也可为研究附生藻的季节变化以及分析生物地理学分布提供重要依据。 展开更多
关键词 秦皇岛 秋季 石花菜(Gelidium amansii) 附生藻 培养 实验分类学 形态学
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四川黄龙钙华沉积区附生藻垫生物与水环境因子相关性分析
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作者 赵晓夏 李琼芳 +5 位作者 代群威 孙庚 张强 车明轩 董鹏举 任亚珍 《微生物学通报》 CAS CSCD 北大核心 2023年第3期924-937,共14页
【背景】近年来,四川省黄龙风景区钙华沉积地貌上藻垫大面积滋生,厚度达3-5cm,严重影响了钙华的持续沉积及欣赏价值。【目的】为有效控制藻类过度生长,并提出相关治理方法措施,需要对藻垫的结构特征及滋生原因进行深入分析。【方法】利... 【背景】近年来,四川省黄龙风景区钙华沉积地貌上藻垫大面积滋生,厚度达3-5cm,严重影响了钙华的持续沉积及欣赏价值。【目的】为有效控制藻类过度生长,并提出相关治理方法措施,需要对藻垫的结构特征及滋生原因进行深入分析。【方法】利用PE-250高通量测序平台对黄龙钙华沉积区典型附生藻垫剖面的不同层次进行真核物种组成分析,同时通过场发射扫描电子显微镜对其微观形貌进行表征,并且对藻垫的生长厚度与水环境参数进行了相关性分析。【结果】藻垫内的真核生物有400种左右,优势藻类主要为硅藻门(Diatomea)、轮虫动物门(Rotifera)和链型植物门(Streptophyta),硅藻门主要为桥弯藻属(Cymbella)。非度量多维尺度分析(non-metric multidimensional scaling,NMDS)统计和聚类分析表明,藻垫的中层和底层真核物种组成具有较高的相似性。场发射扫描电子显微镜的结果显示,硅藻主要分布在藻垫上层,中层主要为丝状藻类,底层为钙华颗粒填充在丝状藻构成的网状结构中。水质参数与藻垫生长的相关性分析表明,藻垫厚度主要与水体中的总氮(total nitrogen, TN)、总磷(total phosphorous, TP)和溶解氧(dissolved oxygen,DO)呈正相关(P<0.05)。【结论】黄龙钙华附生藻垫物种组成较为丰富,但在门水平上较为简单,具有物种组成及微观结构上的分层特性,水体营养化可能是促进藻垫生长的重要原因之一。本研究结果可为黄龙景区藻类滋生治理提供理论基础及依据。 展开更多
关键词 黄龙风景区 钙华 附生藻
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一株产碱性磷酸酶附生菌对微囊藻生长的影响 被引量:6
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作者 赵婕 李建宏 +3 位作者 管章玲 许玲 潘澄 李朋富 《湖泊科学》 EI CAS CSCD 北大核心 2011年第1期49-55,共7页
为研究产碱性磷酸酶(ALP)细菌对微囊藻(Microcystis aeruginosa)从有机磷中获得磷营养的影响,从微囊藻群体中分离获得一株产ALP附生菌Y6,通过ITS分子鉴定该菌属赤杆菌属(Erythrobacter).探讨该菌在有机磷条件下对微囊藻生长及生理代谢... 为研究产碱性磷酸酶(ALP)细菌对微囊藻(Microcystis aeruginosa)从有机磷中获得磷营养的影响,从微囊藻群体中分离获得一株产ALP附生菌Y6,通过ITS分子鉴定该菌属赤杆菌属(Erythrobacter).探讨该菌在有机磷条件下对微囊藻生长及生理代谢的影响,结果表明:(1)细菌发酵液中ALP活性与Y6菌的浓度呈正相关,在培养初期的延滞期即开始产生ALP;(2)在有机磷源条件下Y6的加入对微囊藻生长有促进作用;(3)有机磷源条件下,Y6有助于维持群体微囊藻的上浮率和较大群体,Y6的加入也有维持藻细胞叶绿素荧光强度的作用.本研究的结果表明,产ALP附生菌有助于微囊藻利用水体中有机磷,有利于水华形成. 展开更多
关键词 微囊 附生 碱性磷酸酶 Chl.a荧光强度 水华
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微小亚历山大藻amtk-4共附生菌群多样性及其产毒新种Z1-D的sxtA1基因进化研究 被引量:1
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作者 张若男 田晓清 +4 位作者 陆亚男 樊成奇 张静 杨桥 张晓玲 《海洋渔业》 CSCD 北大核心 2019年第3期364-373,共10页
藻菌关系是揭示赤潮生消与防控、赤潮毒素产生机制的关键,而产毒甲藻共附生菌群多样性及可培养菌株的获得是解析藻菌关系的前提。微小亚历山大藻(Alexandrium minutum)是全球性典型赤潮甲藻,其产生的麻痹性贝类毒素(PSP)危害巨大,但目... 藻菌关系是揭示赤潮生消与防控、赤潮毒素产生机制的关键,而产毒甲藻共附生菌群多样性及可培养菌株的获得是解析藻菌关系的前提。微小亚历山大藻(Alexandrium minutum)是全球性典型赤潮甲藻,其产生的麻痹性贝类毒素(PSP)危害巨大,但目前对其共附生菌群尚缺乏系统性研究。通过高通量测序首次解析了产毒微小亚历山大藻amtk-4共附生菌群的物种种类及相对丰度信息;分离获得可培养菌株并对筛选获得的产毒细菌新种Z1-D的毒素合成基因sxtA1进行了基因进化分析,结果表明,amtk-4共附生菌群包括85个OTU,其中包括10门、20纲、40目、59科及87属。其6个优势属包括Phycisphaeraceae科未知属(11.8%)、Muricauda属(10.3%)、腐螺旋菌科未鉴定属(9.1%)、Hyphomonadaceae科未鉴定属(8.9%)、Haliea属(5.7%)及红细菌科未鉴定属(5.1%)。amtk-4共附生菌群中未鉴定属比例高达53.4%。藻生长稳定期所分离获得的可培养细菌数量及种类最多。5株细菌中菌株Z1-D及Z1-4经分子鉴定分别为亚硫酸杆菌属(Sulfitobacter)及Mesorhizobium属新种。其中Z1-D发酵代谢产物含微量石房蛤毒素(STX),其基因片段orf-01498与蓝藻sxtA1基因高度同源,与产毒蓝藻间可能存在着基因共同进化。 展开更多
关键词 微小亚历山大 麻痹性贝类毒素 附生 生物多样性 sxtA1基因
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微小卡罗藻(Karlodinium micrum)共附生微生物抗菌与细胞毒活性 被引量:3
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作者 朱鹏 游玉容 +2 位作者 褚椒江 金海晓 严小军 《微生物学报》 CAS CSCD 北大核心 2010年第8期1044-1050,共7页
【目的】本文对微小卡罗藻共附生微生物进行分离并对其抗菌和细胞毒活性进行初步研究,以期望获得既具有抗菌又具有细胞毒的高活性菌株,为从共附生微生物的角度去研究微小卡罗藻毒素的合成途径以及真正来源提供研究材料。【方法】利用琼... 【目的】本文对微小卡罗藻共附生微生物进行分离并对其抗菌和细胞毒活性进行初步研究,以期望获得既具有抗菌又具有细胞毒的高活性菌株,为从共附生微生物的角度去研究微小卡罗藻毒素的合成途径以及真正来源提供研究材料。【方法】利用琼脂扩散法和MTT法对细菌培养液的乙酸乙酯提取物进行抗菌和细胞毒活性筛选,并对具有细胞毒活性的细菌菌株进行了16S rRNA系统发生学分析。【结果】在分离到的38株海洋细菌中,25株细菌具有抗菌活性,5株细菌(W-14-2、W-2-2、W-12、E-8-2和W-4)具有细胞毒活性。对这5株具有细胞毒活性的细菌菌株进行16S rRNA系统发生学分析显示它们分别与Alteromonas alvinellae、Stappia aggregata、Pelagibaca bermudensis、Marinobacter kribbensis和Maribacter dokdonensis的16S rRNA基因序列具有较高的相似性。【结论】在分离到的微小卡罗藻共附生微生物中含有较为丰富的活性菌株,且获得5株具有抗菌活性又具有细胞毒的高活性菌株。 展开更多
关键词 卡罗附生微生物 抗菌活性 细胞毒活性 16S RRNA
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A new record from China of epiphytic marine algae,Acrochaete leptochaete(Chaetophoraceae,Chlorophyta)with its primary experimental biology 被引量:1
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作者 邓蕴彦 汤晓荣 +1 位作者 丁兰平 连绍兴 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第2期350-355,共6页
Acrochaete leptochaete, a species in Chaetophoraceae (Chlorophyta), was observed during our recent laboratory culture of the macroalgae Chaetornorpha that was originally collected from an intertidal pool in Rongchen... Acrochaete leptochaete, a species in Chaetophoraceae (Chlorophyta), was observed during our recent laboratory culture of the macroalgae Chaetornorpha that was originally collected from an intertidal pool in Rongcheng, Shandong Province, China. This is the first record of this species in China. Its morphology, taxonomy, and distribution were introduced and discussed in detail. Isolated culture experiments at different temperatures (9-29℃) and light intensities (36-108 μmol/m^2.s) were also carried out. The culture-based observations have extended our knowledge of growth morphology and general biology of the species. 展开更多
关键词 Acroehaete leptochaete China GROWTH MORPHOLOGY new record TAXONOMY
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Periodic exposure to ambient solar irradiance benefits the growth of juvenile seedlings of Hizikia fusiformis
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作者 单体锋 逄少军 高素芹 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第5期1009-1014,共6页
In our trials, from 2007 to 2008, of mass production of seedlings of Hizikiafusiformis using synchronization techniques, problems of a "dark thalli" phenomenon and epiphytes contamination severely threatened the hea... In our trials, from 2007 to 2008, of mass production of seedlings of Hizikiafusiformis using synchronization techniques, problems of a "dark thalli" phenomenon and epiphytes contamination severely threatened the health of juvenile seedlings. In this investigation, we optimized conditions for improving the growth of juvenile seedlings. Seven string collectors were seeded with zygotes and a series of experiments were conducted including direct exposure to solar irradiance, co-culture with Ulva spp. and treatment with sodium hypochlorite. It was found that direct exposure to solar irradiance (maximum: 1 740 μmol photons/(m2.s)) for 2 h per day could efficiently enhance the growth of young seedlings and simultaneously inhibit the growth of epiphytic algae. In this treatment, 50-day old seedlings could reach an average of 0.44 cm in length and possess up to nine leaflets. However, a single treatment with 18-mmol/L sodium hypochlorite for 10 rain severely harmed 15-day old seedlings. In comparison, weekly treatment with 2.2-mmol/L sodium hypochlorite for 10 rain brought no apparent harm to seedlings and eliminated epiphytic algae efficiently. However, this treatment significantly increased the detachment rate of seedlings, Inoculating Ulva spp. onto the collector caused a dramatic decrease in the number of seedlings. However, the growth of the remaining seedlings appeared unhampered. All collectors except the control were daily sprayed with a high pressure water jet from the 84 day post fertilization. From the first day to 50th day, no "dark thallus" was observed on any of the seven collectors. We believe that well-timed daily exposure to solar irradiance would favor H. fusiformis in its early growing stages. 展开更多
关键词 Hizikiafusiformis solar irradiance dark thalli EPIPHYTES resistance
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Removal of Heavy Metal Ions from Wastewater by Using Biosorbents from Marine Algae-A Cost Effective New Technology 被引量:2
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作者 虞启明 PairatKaewsarn +2 位作者 马卫东 JoseT.Matheickal 尹平河 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2001年第2期133-136,共4页
Heavy metal pollution from industrial wastewater is a worldwide environmental issue. Biosorption of heavy metals by using biosorbents derived from various types of biomass has been shown to be effective for the uptake... Heavy metal pollution from industrial wastewater is a worldwide environmental issue. Biosorption of heavy metals by using biosorbents derived from various types of biomass has been shown to be effective for the uptake of heavy metal ions. In this study, biosorbents derived from the biomass of a group of marine macroalgae were used for the removal and recovery of heavy metal ions from aqueous solutions. Results indicated that the biosorbents have high uptake capacities and affinities for a number of heavy metal ions. The uptake capacities of the biosorbents were in the range of 1.0 to 1.5mmol·g-1 for divalent heavy metal ions. The kinetics of the uptake process was fast and the process can be used in both batch and fixed-bed operations. It appears that the biosorption process by using biosorbents from marine macroalgae can be an efficient and cost effective technology for the treatment of heavy metal containing wastewater. 展开更多
关键词 biosorption of heavy metal BIOSORBENT marine algae wastewater treatment
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Biosorption of cadmium(Ⅱ) from aqueous solutions by industrial fungus Rhizopus cohnii 被引量:5
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作者 罗金明 肖潇 罗胜联 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第6期1104-1111,共8页
An important filamentous industrial fungus,Rhizopus cohnii(R.cohnii),was used as an efficient biosorbent for removing cadmium from wastewater.The sorption conditions,such as pH,the dose of biomass and the initial conc... An important filamentous industrial fungus,Rhizopus cohnii(R.cohnii),was used as an efficient biosorbent for removing cadmium from wastewater.The sorption conditions,such as pH,the dose of biomass and the initial concentration of cadmium were examined.Two kinds of adsorption models were applied to simulate the biosorption data.The uptake of cadmium was higher in weak acid condition than in strong acid condition.Nearly no sorption of cadmium occurred when the pH value was lower than 2.0. Biosorption isothermal data could be well simulated by both Langmuir and Freundlich models.Langmuir simulation of the biosorption showed that the maximum uptake of cadmium was 40.5 mg/g(0.36 mmol/g)in the optimal conditions,which was higher than many other adsorbents,including biosorbents and activated carbon.In addition,the reusability results showed that after five times of sorption and desorption process,the sorption capacity of R.cohnii could still maintain nearly 80%,confirming its practical application in cadmium treatment.Fourier transform infrared spectrum revealed that carboxyl,amino and hydroxyl groups on biosorbent R.cohnii surface were responsible for the biosorption of cadmium. 展开更多
关键词 BIOSORPTION Rhizopus cohnii BIOSORBENT CADMIUM
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Thermodynamic Analysis of Heavy Metals Biosorption by Two Macroalgae 被引量:1
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作者 陈丽丹 王宪 +1 位作者 徐鲁荣 钱爱红 《Marine Science Bulletin》 CAS 2004年第1期47-52,共6页
The thermodynamic process of two macroalgae, Sargassum fusiforme and Laminaria japonica, absorbing heavy metal ions Cu2+, Pb2+, Cd2+ and Ni2+, has been studied. The result indicates that the absorption isotherms of th... The thermodynamic process of two macroalgae, Sargassum fusiforme and Laminaria japonica, absorbing heavy metal ions Cu2+, Pb2+, Cd2+ and Ni2+, has been studied. The result indicates that the absorption isotherms of these two macroalgae clearly accord with the Landmuir adsorption model. The absorptive processes of S. fusiforme and L. japonica for Cu2+℃ are endothermal, and at 35 , the adsorption heat of these two algae is 59.5 kJ/mol and 76.8 kJ/mol respectively. Temperature could affect the algae’s adsorption capacity. Their adsorption ℃℃capacity increases with temperature ( 25 and 35 ). 展开更多
关键词 thermodynamics of biosorption adsorption capacity heavy metal MACROALGAE
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