Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vi...Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.展开更多
This review focused on the recent reports related to the function, characterization and modification of oxygen-containing surface groups of activated carbon (AC). The Oxygen-containing surface groups were briefly desc...This review focused on the recent reports related to the function, characterization and modification of oxygen-containing surface groups of activated carbon (AC). The Oxygen-containing surface groups were briefly described, and the most frequently used techniques for characterization of the oxygen-containing surface groups on ACs were also briefly stated. A detailed discussion of the effects of the oxygen-containing surface groups on the adsorptive capacity of AC was given. The recent progresses in modification of the oxygen-containing surface groups of AC were also reviewed.展开更多
Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modific...Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modifications of proteins with phosphates, lipids, sugars or other residues confer on these proteins additional structural and functional diversity. For instance, protein phosphorylation is involved in a wide range of cellular processes including signal transduction. Protein glycosylation is one of the most abundant PTMs and more than 50% of all human proteins are glycosylated. Glycoproteins are involved in many biological events, such as cell-cell adhesion, communication, immune response and development.展开更多
基金Supported by the National Natural Science Foundation of China(30800479)
文摘Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.
基金National Natural Science Foundation of China (No. 20336020) and Science Foundation of Guangdong Province of China (2002C32103).
文摘This review focused on the recent reports related to the function, characterization and modification of oxygen-containing surface groups of activated carbon (AC). The Oxygen-containing surface groups were briefly described, and the most frequently used techniques for characterization of the oxygen-containing surface groups on ACs were also briefly stated. A detailed discussion of the effects of the oxygen-containing surface groups on the adsorptive capacity of AC was given. The recent progresses in modification of the oxygen-containing surface groups of AC were also reviewed.
文摘Recently, there has been an overwhelming demand for studies on protein post-translational modification （PTM） to understand the increasing complexity from the level of the genome to the proteome. The covalent modifications of proteins with phosphates, lipids, sugars or other residues confer on these proteins additional structural and functional diversity. For instance, protein phosphorylation is involved in a wide range of cellular processes including signal transduction. Protein glycosylation is one of the most abundant PTMs and more than 50% of all human proteins are glycosylated. Glycoproteins are involved in many biological events, such as cell-cell adhesion, communication, immune response and development.
