Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coast...Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coastal water(Pearl River Estuary), South China Sea, at 12 sites(S1–S12) were explored by community-level physiological profiling(CLPP) with BIOLOG Eco-plate and denaturing gradient gel electrophoresis(DGGE). Our results showed that the core mariculture area(S6, S7 and S8) and the sites associating with human activity and sewage discharge(S11 and S12) had higher microbial metabolic capability and bacterial community diversity than others(S1–5, S9–10). Especially, the diversity index of S11 and S12 calculated from both CLPP and DGGE data(H >3.2) was higher than that of others as sewage discharge may increase water nitrogen and phosphorus nutrient. The bacterial community structure of S6, S8, S11 and S12 was greatly influenced by total phosphorous, salinity and total nitrogen. Based on DGGE fingerprinting, proteobacteria, especially γ- and α-proteobacteria, were found dominant at all sites. In conclusion, the aquaculture area and wharf had high microbial metabolic capability. The structure and composition of bacterial community were closely related to the level of phosphorus, salinity and nitrogen.展开更多
THE almost two-meter-high sculpture Liu Xiaofan admired at an exhibition,and which he assumed was bronze,turned out to have been made from recycled building waste.Liu immediately thought of the pile of demolition debr...THE almost two-meter-high sculpture Liu Xiaofan admired at an exhibition,and which he assumed was bronze,turned out to have been made from recycled building waste.Liu immediately thought of the pile of demolition debris next to his home.He had witnessed new construction waste steadily piling up next to his apartment building and the community public lawn.Dust floated high and free as the wind blew and deposited it on his balcony.This epitomizes the "metabolism" of Chinese cities.展开更多
基金supported by the Support Project of Science and Technology of China (Grant No. 2012BAD18B01)Special Fund for Agro-scientific Research in the Public Interest (201403008)+1 种基金the Natural Science Foundation of China (Grant Nos. U1301235, 41173079)special scientific research funds for central non-profit institutes,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (2014TS04)
文摘Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coastal water(Pearl River Estuary), South China Sea, at 12 sites(S1–S12) were explored by community-level physiological profiling(CLPP) with BIOLOG Eco-plate and denaturing gradient gel electrophoresis(DGGE). Our results showed that the core mariculture area(S6, S7 and S8) and the sites associating with human activity and sewage discharge(S11 and S12) had higher microbial metabolic capability and bacterial community diversity than others(S1–5, S9–10). Especially, the diversity index of S11 and S12 calculated from both CLPP and DGGE data(H >3.2) was higher than that of others as sewage discharge may increase water nitrogen and phosphorus nutrient. The bacterial community structure of S6, S8, S11 and S12 was greatly influenced by total phosphorous, salinity and total nitrogen. Based on DGGE fingerprinting, proteobacteria, especially γ- and α-proteobacteria, were found dominant at all sites. In conclusion, the aquaculture area and wharf had high microbial metabolic capability. The structure and composition of bacterial community were closely related to the level of phosphorus, salinity and nitrogen.
文摘THE almost two-meter-high sculpture Liu Xiaofan admired at an exhibition,and which he assumed was bronze,turned out to have been made from recycled building waste.Liu immediately thought of the pile of demolition debris next to his home.He had witnessed new construction waste steadily piling up next to his apartment building and the community public lawn.Dust floated high and free as the wind blew and deposited it on his balcony.This epitomizes the "metabolism" of Chinese cities.
