目的探讨早期高压氧、低气压干预对低气压环境大鼠降钙素基因相关蛋白（calcitonin gene related protein，CGRP）和内皮素（endothelin，ET）的影响，对比研究两种方法的作用。方法62只Wistar大鼠随机分成6组：（1）对照组，（2）海拔3...目的探讨早期高压氧、低气压干预对低气压环境大鼠降钙素基因相关蛋白（calcitonin gene related protein，CGRP）和内皮素（endothelin，ET）的影响，对比研究两种方法的作用。方法62只Wistar大鼠随机分成6组：（1）对照组，（2）海拔3800m（2h）组，（3）海拔3800m（3d）组，（4）海拔3800m（7d）组，（5）高压氧干预组，（6）低气压环境干预组。首先把（2）、（3）、（4）实验组大鼠置于低压氧舱中，匀速减压使舱内压力由海平面0．1MPa降至0．062MPa，相当于海拔3800m的高原低气压环境，在2h、3d、7d时间点断头取血备检；对（5）组大鼠先期给予高压氧处理（0．2MPa，1次／d，吸氧60min，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；对（6）组大鼠先期给予低气压缺氧处理（0．062MPa，1次／d，2h／次，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；将上述不同处理方法所得血样离心取上清，放免法测定CGRP、ET浓度。结果与对照组比较，CGRP浓度随着大鼠在低气压环境中的时间延长有逐渐减少趋势，但差异无统计学意义，高压氧预处理对CGRP无影响，低气压缺氧预处理虽然使CGRP浓度下降趋势更明显，但差异无统计学意义。ET浓度随着时间的延长有逐渐增加趋势，但也无统计学意义。高压氧[（305．26±39．66）μg/L]、低气压缺氧预处理[（309．18±25．07）μg/L]均使ET浓度明显升高，差异有统计学意义（P〈0．05）。结论高压氧、低气压缺氧早期干预主要影响大鼠ET的变化，高压氧预处理可能具有增强机体高原适廊能力的作用。展开更多
Intermedin(IMD)or adrenomedullin 2 is a novel peptide related to the calcitonin gene-related peptide(CGRP)family.Via calcitonin receptor-like receptor/receptor activity modifying proteins,the common receptor complexes...Intermedin(IMD)or adrenomedullin 2 is a novel peptide related to the calcitonin gene-related peptide(CGRP)family.Via calcitonin receptor-like receptor/receptor activity modifying proteins,the common receptor complexes of CGRP,IMD exerts a wide range of biological effects,especially regulation of cardiovascular homeostasis.Proteolytic processing of a larger IMD precursor yields a series of biologically active C-terminal fragments,IMD1–53,IMD1–47 and IMD8–47.IMD and its receptors are present in the cardiovascular system,and IMD is present at low levels in plasma.In the cardiovascular system,IMD has multiple functions such as regulation of blood pressure and cardiac function,pro-angiogenesis,endothelial barrier function protection,anti-oxidative stress,and anti-endoplasmic reticulum stress.IMD participates widely in the pathogenesis of atherosclerosis,hypertension,pulmonary arterial hypertension and vascular calcification.It is a vascular regulatory factor of homeostasis and a vital endogenous protective factor against vascular diseases.展开更多
文摘目的探讨早期高压氧、低气压干预对低气压环境大鼠降钙素基因相关蛋白（calcitonin gene related protein，CGRP）和内皮素（endothelin，ET）的影响，对比研究两种方法的作用。方法62只Wistar大鼠随机分成6组：（1）对照组，（2）海拔3800m（2h）组，（3）海拔3800m（3d）组，（4）海拔3800m（7d）组，（5）高压氧干预组，（6）低气压环境干预组。首先把（2）、（3）、（4）实验组大鼠置于低压氧舱中，匀速减压使舱内压力由海平面0．1MPa降至0．062MPa，相当于海拔3800m的高原低气压环境，在2h、3d、7d时间点断头取血备检；对（5）组大鼠先期给予高压氧处理（0．2MPa，1次／d，吸氧60min，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；对（6）组大鼠先期给予低气压缺氧处理（0．062MPa，1次／d，2h／次，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；将上述不同处理方法所得血样离心取上清，放免法测定CGRP、ET浓度。结果与对照组比较，CGRP浓度随着大鼠在低气压环境中的时间延长有逐渐减少趋势，但差异无统计学意义，高压氧预处理对CGRP无影响，低气压缺氧预处理虽然使CGRP浓度下降趋势更明显，但差异无统计学意义。ET浓度随着时间的延长有逐渐增加趋势，但也无统计学意义。高压氧[（305．26±39．66）μg/L]、低气压缺氧预处理[（309．18±25．07）μg/L]均使ET浓度明显升高，差异有统计学意义（P〈0．05）。结论高压氧、低气压缺氧早期干预主要影响大鼠ET的变化，高压氧预处理可能具有增强机体高原适廊能力的作用。
基金supported by the National Natural Science Foundation of China(91339203,81270407,81170082 to Qi YongFen)
文摘Intermedin(IMD)or adrenomedullin 2 is a novel peptide related to the calcitonin gene-related peptide(CGRP)family.Via calcitonin receptor-like receptor/receptor activity modifying proteins,the common receptor complexes of CGRP,IMD exerts a wide range of biological effects,especially regulation of cardiovascular homeostasis.Proteolytic processing of a larger IMD precursor yields a series of biologically active C-terminal fragments,IMD1–53,IMD1–47 and IMD8–47.IMD and its receptors are present in the cardiovascular system,and IMD is present at low levels in plasma.In the cardiovascular system,IMD has multiple functions such as regulation of blood pressure and cardiac function,pro-angiogenesis,endothelial barrier function protection,anti-oxidative stress,and anti-endoplasmic reticulum stress.IMD participates widely in the pathogenesis of atherosclerosis,hypertension,pulmonary arterial hypertension and vascular calcification.It is a vascular regulatory factor of homeostasis and a vital endogenous protective factor against vascular diseases.
