Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refe...Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refer to the study of mitotical/meiotical heritage changes in gene function that cannot be explained by changes in the DNA sequence. Major epigenetic mechanisms include DNA methylation, histone modification, chromatin remodeling, genomic imprinting, and non-coding RNA. In this review, we focus on the new insights into the epigenetic mechanism for neural stem cells fate.展开更多
Large vertical steel tanks for fluid storage are usually constructed on soft foundations, so it is not surprising that the tank wall will settle unevenly with the settlement of the foundation, thus inducing deformatio...Large vertical steel tanks for fluid storage are usually constructed on soft foundations, so it is not surprising that the tank wall will settle unevenly with the settlement of the foundation, thus inducing deformations and stresses in the tank. This work investigates the linear static behavior of floating-roof tanks under harmonic settlement through finite element (FE) analyses. The influences of the radius-to-thickness ratio, the height-to-radius ratio and the wind girder stiffness on the structural behavior are first analyzed. Comparisons between the circumferential stresses in the wind girder and the vertical stresses in the tank bottom are then made. The displacement and the stress along the tank height are also discussed, and the concept of tank division along its height is presented. Finally, a design approximation for the radial displacement at the tank top is developed based on FE results, and a settlement criterion based on the top radial displacement is proposed which can be used in practical design.展开更多
The application of the finite layer & triangular prism element method to the 3D ground subsidence and stress analysis caused by mining is presented. The layer elements and the triangular prism elements have been a...The application of the finite layer & triangular prism element method to the 3D ground subsidence and stress analysis caused by mining is presented. The layer elements and the triangular prism elements have been alternatively used in the numerical simulation system, the displacement pattern, strain matrix, elastic matrix, stiffness matrix, load matrix and the stress matrix of the layer element and triangular prism element have been presented. By means of the Fortran90 programming language, a numerical simulation system based on finite layer & triangular prism element have been built up, and this system is suitable for subsidence prediction and stress analysis of all mining condition and mining methods. Comparing with the infinite element method, this approach dramatically reduces the size of the set of equations that need to be solved, and greatly reduces the amount of data preparation required. It not only saves the internal storage, and the computation time, but also decreases the cost.展开更多
The proliferation of Hoek-Brown nonlinear failure criterion and upper bound theorem makes it possible to evaluate the stability of circular tunnels with an original curved collapsing mechanism. The arch effect of shal...The proliferation of Hoek-Brown nonlinear failure criterion and upper bound theorem makes it possible to evaluate the stability of circular tunnels with an original curved collapsing mechanism. The arch effect of shallow circle tunnel is not taken into consideration so that the mechanical characteristics can be easily described. Based on the mechanism, the upper bound solution of supporting pressure of tunnels under the condition of surface settlements and overloads on the ground surface is derived. The objective function is formed from virtual work equations under the variational principle, and solutions are presented by the optimum theory. Comparisons with previous works are made. The numerical results of the present method show great agreement with those of existing ones. With regard to the surface settlement and overloads, the influence of different rock parameters on the collapsing shape is analyzed.展开更多
Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method...Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method. Top-down excavation of the Metro Line 10 in Shanghai was modeled with finite element analysis software ABAQUS and parameters of subsoil were obtained by inverse analysis. Based on the finite element model and parameters, changes in the following factors were made to find more effective methods to restrain differential uplift and settlement: length of diaphragm wall, thickness of jet-grouting reinforcement layer, ways of subsoil reinforcement, sequence of pit excavation, connection between slabs and diaphragm wall or column and width of pit. Several significant results are acquired. The longer the diaphragm wall is, the greater the differential uplift between column and diaphragm wall is. Rigidity of roof slab is in general not strong enough to keep diaphragm wall and column undergoing the same uplift during excavation; Uplift at head of column and differential uplift between column and diaphragm wall decrease when subsoil from-16.6 to-43 m in pit is reinforced through jet-grouting. But, as excavation proceeds to a lower level, benefit from soil reinforcement diminishes. During the process applying vertical load, the larger the depth of diaphragm wall is, the smaller the settlement is at head of column and diaphragm wall, and the greater the differential settlement is between column and diaphragm wall. When friction connection is implemented between column, diaphragm wall and floor slabs, uplifts at head of column and diaphragm wall are larger than those of the case when tie connection is implemented, and so does differential uplift between column and diaphragm wall. The maximum deflection of diaphragm wall decreases by 58% on account of soil reinforcement in pit. The maximum deflection of diaphragm wall decreases by 61.2% when friction connection is implemented instead of tie connection.展开更多
The objective of this study is to incorporate a numerical model with GIS to simulate the movement, erosion and deposition of debris flow across the three dimensional complex terrain. In light of the importance of eros...The objective of this study is to incorporate a numerical model with GIS to simulate the movement, erosion and deposition of debris flow across the three dimensional complex terrain. In light of the importance of erosion and deposition processes during debris flow movement, no entrainment assumption is unreasonable. The numerical model considering these processes is used for simulating debris flow. Raster grid networks of a digital elevation model in GIS provide a uniform grid system to describe complex topography. As the raster grid can be used as the finite difference mesh, the numerical model is solved numerically using the Leap-frog finite difference method. Finally, the simulation results can be displayed by GIS easily and used to debris flow evaluation. To illustrate this approach, the proposed methodology is applied to the Yohutagawa debris flow that occurred on 2oth October 2010, in Amami- Oshima area, Japan. The simulation results that reproduced the movement, erosion and deposition are in good agreement with the field investigation. The effectiveness of the dam in this real-ease is also verified by this approach. Comparison with the results were simulated by other models, shows that the present coupled model is more rational and effective.展开更多
Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We inv...Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes: Vallisneria natans and Hydrilla verticillata in Taihu Lake, Jiangsu, China, using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes. The results show that: (1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria, and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria, including Burkholderiales, was the most abundant bacterial group on both plants. Cyanobacteria and Gammaproteobacteria were the second largest groups on V. natans and H. verticillata, respectively. Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations. Both ANOSIM of the T-RFLP data and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants. Therefore, the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific, which may aid in understanding the ecological functions of submerged macrophytes in general.展开更多
With the increase of axle load and the train speed, dynamic interaction of train-track system becomes so exacerbated that the deformation and dynamic response of subgrade are more aggravated. The differential settleme...With the increase of axle load and the train speed, dynamic interaction of train-track system becomes so exacerbated that the deformation and dynamic response of subgrade are more aggravated. The differential settlement will be created in bridge-embankment transition section under such dynamic action, and an adverse effect on the train operation safety can be caused. Meanwhile, differential settlement will produce additional dynamic effect when high-speed trains go through the transition between bridge-embankment. Such dynamic action will aggravate the differential settlement and subgrade damage. This paper applies the methods of field test and finite-element to systematically study the dynamic response characteristics of subgrade in bridge-embankment transition section of heavy haul railway under dynamic load for the first time. This research is focused on the analysis of influence of the different axle load, train speed, filled soil modulus, etc.. At last, the dynamic response rules are systematically summarized.展开更多
In this study,based on the dynamic Biot's theory "u-p" approximation,a 3D finite element method(FEM) numerical soil model is developed,in which the Generalized Newmark-β method is adopted to determine the time i...In this study,based on the dynamic Biot's theory "u-p" approximation,a 3D finite element method(FEM) numerical soil model is developed,in which the Generalized Newmark-β method is adopted to determine the time integration.The developed 3D FEM soil model is a part of the coupled model PORO-WSSI 3D for 3D wave-seabed-marine structures interaction problem,and is validated by the analytical solution proposed by Wang(2000) for a laterally infinite seabed loaded by a uniform force.By adopting the developed 3D soil model,the consolidation of seabed under a caisson breakwater and hydrostatic pressure is investigated.The numerical results show that the caisson breakwater built on seabed has very significant effect on the stresses/displacements fields in the seabed foundation after the transient deformation and primary consolidation are completed.The parametric study indicates that the Young's modulus E of seabed is the most important parameter to affect the settlement of breakwater,and the displacement fields in seabed foundation.Taking the consolidation status as the initial condition,the interaction between ocean wave,caisson breakwater and seabed foundation is briefly investigated.The 3D ocean wave is determined by solving the Navier-Stokes equations with finite volume method(FVM).The numerical results indicate that there is intensive interaction between oceean wave, caisson breakwater and seabed foundation; and the breakwater indeed can effectively block the wave energy propagating to the coastline.展开更多
文摘Neural stem cell is presently the research hotspot in neuroscience. Recent progress indicates that epigenetic modulation is closely related to the self-renewal and differentiation of neural stem cell. Epigenetics refer to the study of mitotical/meiotical heritage changes in gene function that cannot be explained by changes in the DNA sequence. Major epigenetic mechanisms include DNA methylation, histone modification, chromatin remodeling, genomic imprinting, and non-coding RNA. In this review, we focus on the new insights into the epigenetic mechanism for neural stem cells fate.
基金Project supported by the National Natural Science Foundation of China (No. 50208017) and the Scientific Research Foundation forReturned Overseas Chinese Scholars, Ministry of Education (No.J20040157), China
文摘Large vertical steel tanks for fluid storage are usually constructed on soft foundations, so it is not surprising that the tank wall will settle unevenly with the settlement of the foundation, thus inducing deformations and stresses in the tank. This work investigates the linear static behavior of floating-roof tanks under harmonic settlement through finite element (FE) analyses. The influences of the radius-to-thickness ratio, the height-to-radius ratio and the wind girder stiffness on the structural behavior are first analyzed. Comparisons between the circumferential stresses in the wind girder and the vertical stresses in the tank bottom are then made. The displacement and the stress along the tank height are also discussed, and the concept of tank division along its height is presented. Finally, a design approximation for the radial displacement at the tank top is developed based on FE results, and a settlement criterion based on the top radial displacement is proposed which can be used in practical design.
文摘The application of the finite layer & triangular prism element method to the 3D ground subsidence and stress analysis caused by mining is presented. The layer elements and the triangular prism elements have been alternatively used in the numerical simulation system, the displacement pattern, strain matrix, elastic matrix, stiffness matrix, load matrix and the stress matrix of the layer element and triangular prism element have been presented. By means of the Fortran90 programming language, a numerical simulation system based on finite layer & triangular prism element have been built up, and this system is suitable for subsidence prediction and stress analysis of all mining condition and mining methods. Comparing with the infinite element method, this approach dramatically reduces the size of the set of equations that need to be solved, and greatly reduces the amount of data preparation required. It not only saves the internal storage, and the computation time, but also decreases the cost.
基金Project(2013CB036004)supported by the National Basic Research Program of ChinaProjects(51178468,51378510)supported by the National Natural Science Foundation of China
文摘The proliferation of Hoek-Brown nonlinear failure criterion and upper bound theorem makes it possible to evaluate the stability of circular tunnels with an original curved collapsing mechanism. The arch effect of shallow circle tunnel is not taken into consideration so that the mechanical characteristics can be easily described. Based on the mechanism, the upper bound solution of supporting pressure of tunnels under the condition of surface settlements and overloads on the ground surface is derived. The objective function is formed from virtual work equations under the variational principle, and solutions are presented by the optimum theory. Comparisons with previous works are made. The numerical results of the present method show great agreement with those of existing ones. With regard to the surface settlement and overloads, the influence of different rock parameters on the collapsing shape is analyzed.
基金Projects(51208071,51108312) supported by the National Natural Science Foundation of China
文摘Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method. Top-down excavation of the Metro Line 10 in Shanghai was modeled with finite element analysis software ABAQUS and parameters of subsoil were obtained by inverse analysis. Based on the finite element model and parameters, changes in the following factors were made to find more effective methods to restrain differential uplift and settlement: length of diaphragm wall, thickness of jet-grouting reinforcement layer, ways of subsoil reinforcement, sequence of pit excavation, connection between slabs and diaphragm wall or column and width of pit. Several significant results are acquired. The longer the diaphragm wall is, the greater the differential uplift between column and diaphragm wall is. Rigidity of roof slab is in general not strong enough to keep diaphragm wall and column undergoing the same uplift during excavation; Uplift at head of column and differential uplift between column and diaphragm wall decrease when subsoil from-16.6 to-43 m in pit is reinforced through jet-grouting. But, as excavation proceeds to a lower level, benefit from soil reinforcement diminishes. During the process applying vertical load, the larger the depth of diaphragm wall is, the smaller the settlement is at head of column and diaphragm wall, and the greater the differential settlement is between column and diaphragm wall. When friction connection is implemented between column, diaphragm wall and floor slabs, uplifts at head of column and diaphragm wall are larger than those of the case when tie connection is implemented, and so does differential uplift between column and diaphragm wall. The maximum deflection of diaphragm wall decreases by 58% on account of soil reinforcement in pit. The maximum deflection of diaphragm wall decreases by 61.2% when friction connection is implemented instead of tie connection.
基金finanicial support from the Global Environment Research Fund of Japan(S-8)from Grants-in-Aid for Scientific Research(Scientific Research(B),22310113,G.Chen)from Japan Society for the Promotion of Science
文摘The objective of this study is to incorporate a numerical model with GIS to simulate the movement, erosion and deposition of debris flow across the three dimensional complex terrain. In light of the importance of erosion and deposition processes during debris flow movement, no entrainment assumption is unreasonable. The numerical model considering these processes is used for simulating debris flow. Raster grid networks of a digital elevation model in GIS provide a uniform grid system to describe complex topography. As the raster grid can be used as the finite difference mesh, the numerical model is solved numerically using the Leap-frog finite difference method. Finally, the simulation results can be displayed by GIS easily and used to debris flow evaluation. To illustrate this approach, the proposed methodology is applied to the Yohutagawa debris flow that occurred on 2oth October 2010, in Amami- Oshima area, Japan. The simulation results that reproduced the movement, erosion and deposition are in good agreement with the field investigation. The effectiveness of the dam in this real-ease is also verified by this approach. Comparison with the results were simulated by other models, shows that the present coupled model is more rational and effective.
基金Supported by the National Natural Science Foundation of China(No.40730528)the National Basic Research Program of China(973Program)(No.2008CB418104)+2 种基金the Knowledge Innovation Project of Chinese Academy of Sciences(No.KZCX2-YW-JC302)the Jiangsu Provincial Science Foundation(No.BK2009024)the Frontier Foundation of Nanjing Institute of Geography & Limnology,Chinese Academy of Sciences(No.09SL021001)
文摘Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes: Vallisneria natans and Hydrilla verticillata in Taihu Lake, Jiangsu, China, using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes. The results show that: (1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria, and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria, including Burkholderiales, was the most abundant bacterial group on both plants. Cyanobacteria and Gammaproteobacteria were the second largest groups on V. natans and H. verticillata, respectively. Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations. Both ANOSIM of the T-RFLP data and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants. Therefore, the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific, which may aid in understanding the ecological functions of submerged macrophytes in general.
文摘With the increase of axle load and the train speed, dynamic interaction of train-track system becomes so exacerbated that the deformation and dynamic response of subgrade are more aggravated. The differential settlement will be created in bridge-embankment transition section under such dynamic action, and an adverse effect on the train operation safety can be caused. Meanwhile, differential settlement will produce additional dynamic effect when high-speed trains go through the transition between bridge-embankment. Such dynamic action will aggravate the differential settlement and subgrade damage. This paper applies the methods of field test and finite-element to systematically study the dynamic response characteristics of subgrade in bridge-embankment transition section of heavy haul railway under dynamic load for the first time. This research is focused on the analysis of influence of the different axle load, train speed, filled soil modulus, etc.. At last, the dynamic response rules are systematically summarized.
基金the financial support from EPSRC #EP/ G006482/1the funding support of Oversea Research Student Award from Scottish Government, UK
文摘In this study,based on the dynamic Biot's theory "u-p" approximation,a 3D finite element method(FEM) numerical soil model is developed,in which the Generalized Newmark-β method is adopted to determine the time integration.The developed 3D FEM soil model is a part of the coupled model PORO-WSSI 3D for 3D wave-seabed-marine structures interaction problem,and is validated by the analytical solution proposed by Wang(2000) for a laterally infinite seabed loaded by a uniform force.By adopting the developed 3D soil model,the consolidation of seabed under a caisson breakwater and hydrostatic pressure is investigated.The numerical results show that the caisson breakwater built on seabed has very significant effect on the stresses/displacements fields in the seabed foundation after the transient deformation and primary consolidation are completed.The parametric study indicates that the Young's modulus E of seabed is the most important parameter to affect the settlement of breakwater,and the displacement fields in seabed foundation.Taking the consolidation status as the initial condition,the interaction between ocean wave,caisson breakwater and seabed foundation is briefly investigated.The 3D ocean wave is determined by solving the Navier-Stokes equations with finite volume method(FVM).The numerical results indicate that there is intensive interaction between oceean wave, caisson breakwater and seabed foundation; and the breakwater indeed can effectively block the wave energy propagating to the coastline.