目的:以肺炎链球菌鼻腔滴入导致急性上呼吸道感染的小鼠为模型,采用Real-time PCR方法,检测脾脏组织中Toll样受体(TLR)2、TLR4 m RNA的变化,探讨新扶正除疫颗粒中药复方在抗炎及免疫调节方面作用机制。方法:BALB/c小鼠随机分为空白组,...目的:以肺炎链球菌鼻腔滴入导致急性上呼吸道感染的小鼠为模型,采用Real-time PCR方法,检测脾脏组织中Toll样受体(TLR)2、TLR4 m RNA的变化,探讨新扶正除疫颗粒中药复方在抗炎及免疫调节方面作用机制。方法:BALB/c小鼠随机分为空白组,模型组,复方高、中、低剂量组,中药对照组和西药对照组,造模1h后灌药,第1、4、7天为取材时间点,测定小鼠脾组织中脾脏指数及TLR2、TLR4 m RNA表达情况。结果:药物干预后各时间点脾脏指数均有不同程度下降。模型组脾脏TLR2、TLR4 m RNA表达水平相对空白组明显增强(P<0.05),且随感染时间的增加表达量逐渐增强。复方低、中、高剂量组表达水平逐渐下降,复方高剂量组表达水平随感染时间下降趋势最明显。结论:新扶正除疫颗粒可通过调节脾脏TLR2、TLR4 m RNA表达,调控机体抗感染免疫作用,对激活第一道天然免疫的屏障具有一定作用。展开更多
AIM： To elucidate the interaction between non- parenchymal cells, extracellular matrix and oval cells during the restituting process of liver injury induced by partial hepatectomy （PH）. METHODS： We examined the lo...AIM： To elucidate the interaction between non- parenchymal cells, extracellular matrix and oval cells during the restituting process of liver injury induced by partial hepatectomy （PH）. METHODS： We examined the localization of oval cells, non-parenchymal cells, and the extracellular matrix components using immunohistochemical and double immunofluorescent analysis during the proliferation and differentiation of oval cells in N-2- acetylaminofluorene （2-AAF）/PH rat model. RESULTS： By day 2 after PH, small oval cells began to proliferate around the portal area. Most of stellate cells and laminin were present along the hepatic sinusoids in the periportal area. Kupffer cells and fibronectin markedly increased in the whole hepatic Iobule. From day 4 to 9, oval cells spread further into hepatic parenchyma, closely associated with stellate cells, fibronectin and laminin. Kupffer cells admixed with oval cells by day 6 and then decreased in the periportal zone. From day 12 to 15, most of hepatic stellate cells （HSCs）, laminin and fibronectin located around the small hepatocyte nodus, and minority of them appeared in the nodus. Kupffer cells were mainly limited in the pericentral sinusoids. After day 18, the normal liver Iobule structures began to recover.CONCLUSION： Local hepatic microenvironment may participate in the oval cell-mediated liver regeneration through the cell-cell and cell-matrix interactions.展开更多
文摘目的:以肺炎链球菌鼻腔滴入导致急性上呼吸道感染的小鼠为模型,采用Real-time PCR方法,检测脾脏组织中Toll样受体(TLR)2、TLR4 m RNA的变化,探讨新扶正除疫颗粒中药复方在抗炎及免疫调节方面作用机制。方法:BALB/c小鼠随机分为空白组,模型组,复方高、中、低剂量组,中药对照组和西药对照组,造模1h后灌药,第1、4、7天为取材时间点,测定小鼠脾组织中脾脏指数及TLR2、TLR4 m RNA表达情况。结果:药物干预后各时间点脾脏指数均有不同程度下降。模型组脾脏TLR2、TLR4 m RNA表达水平相对空白组明显增强(P<0.05),且随感染时间的增加表达量逐渐增强。复方低、中、高剂量组表达水平逐渐下降,复方高剂量组表达水平随感染时间下降趋势最明显。结论:新扶正除疫颗粒可通过调节脾脏TLR2、TLR4 m RNA表达,调控机体抗感染免疫作用,对激活第一道天然免疫的屏障具有一定作用。
基金Supported by A grant from National Natural Science Foundation of China
文摘AIM： To elucidate the interaction between non- parenchymal cells, extracellular matrix and oval cells during the restituting process of liver injury induced by partial hepatectomy （PH）. METHODS： We examined the localization of oval cells, non-parenchymal cells, and the extracellular matrix components using immunohistochemical and double immunofluorescent analysis during the proliferation and differentiation of oval cells in N-2- acetylaminofluorene （2-AAF）/PH rat model. RESULTS： By day 2 after PH, small oval cells began to proliferate around the portal area. Most of stellate cells and laminin were present along the hepatic sinusoids in the periportal area. Kupffer cells and fibronectin markedly increased in the whole hepatic Iobule. From day 4 to 9, oval cells spread further into hepatic parenchyma, closely associated with stellate cells, fibronectin and laminin. Kupffer cells admixed with oval cells by day 6 and then decreased in the periportal zone. From day 12 to 15, most of hepatic stellate cells （HSCs）, laminin and fibronectin located around the small hepatocyte nodus, and minority of them appeared in the nodus. Kupffer cells were mainly limited in the pericentral sinusoids. After day 18, the normal liver Iobule structures began to recover.CONCLUSION： Local hepatic microenvironment may participate in the oval cell-mediated liver regeneration through the cell-cell and cell-matrix interactions.
