针刺陵下穴治疗肩周炎86例

针刺陵下穴(阳陵泉穴直下2寸凹陷处之压痛点)和针刺陵下穴配合局部按摩治疗肩周炎86例。前者(38例):痊愈25例(65.79％)中,平均治疗4.94次,一次治愈3例(12％),有效率97.37％;后者(48例):痊愈33例(68.75％)中,平均治疗2.79次,一次治愈13例(39.4％),有效率95.83％。经统计学分析表明:二者的疗效均满意,而后者的疗程短,一次治愈率高,较之前者更具先进性。

针刺陵下穴配意念运动治疗扭伤痛症

笔者采用针刺陵下穴配意念、运动治疗扭伤痛症168例，其中1次治愈率40．48％，两次治愈率35．12％，三次以上治愈及好转24．40％，总有效率100％。结论：针刺陵下穴配意念运动治疗扭伤痛症有良好效果。

刺激陵下穴快速缓解胆绞痛188例

刺激陵下穴快速缓解胆绞痛１８８例朱振海（江苏省丹阳市人民医院２１２３００）关键词胆绞痛，针刺疗法，陵下穴，临床应用笔者自１９８４年以来，用按压或针刺陵下穴的方法治疗胆绞痛１８８例，无１例不快速缓解。现报告如下：１一般资料１８８例中，男１１０名，女７８...

针刺陵下穴配合意念运动加滞针法治疗肩关节周围炎49例疗效观察

目的:观察针刺陵下穴配合意念运动加滞针法治疗肩关节周围炎临床疗效。方法:将98例肩关节周围炎患者随机分为2组各49例,治疗组采用针刺陵下穴配合意念运动加滞针法治疗,对照组采用常规针刺方法治疗。2组均治疗2疗程后评价临床疗效,并进行治疗前后肩关节运动功能综合评分和肩部疼痛视觉模拟评分(VAS)比较。结果:总有效率治疗组为93.9%,对照组为81.6%,2组临床疗效及总有效率分别比较,差异均有非常显著性意义(P<0.01)。治疗后2组肩关节运动功能综合评分及肩部VAS评分分别与治疗前比较,差异均有非常显著性意义(P<0.01),且治疗组改善优于对照组,差异均有非常显著性意义(P<0.01)。结论:针刺陵下穴配合意念运动加滞针法治疗肩关节周围炎临床疗效良好。

Acupuncture treatment on 31 cases of obsolete lateral malleolus joint sprain

Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi （解溪 ST 41） were penetrated to Shenmei （申脉 BL 61）, and Yanglingquan （阳陵泉 GB 34） was applied. The needles were retained for 30 min, and the treatment was performed only once, with follow-up visit after 1 month. Results All the patients were cured after treatment, and without relapse on the follow-up visit after 1 month. Conclusion The clinical effect of acupuncture on the obsolete lateral malleolus joint sprain is remarkable.

Effect of acupuncture at complementary acupionts on mast cells and histamine/bradykinin released by mast cells in the regions of acupionts

Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat＇s complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods： Using random number table method, 40 Wistar rats were randomly divided into a blank control group （group K）, an acupuncture at Yanglingquan （GB 34） group （group A）, an acupuncture at Yinlingquan （SP 9） group （group B）, an acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group AB）, and an acupuncture at points near Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group CD）, 8 rats in each group. Group A received acupuncture at Yanglingquan （GB 34）; group B received acupuncture at Yinlingquan （SP 9）; group AB received acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9）; group CD received acupuncture at the control points [points 3 mm away from Yanglingquan （GB 34） and Yinlingquan （SP 9） respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture （EA） device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-linked immunosorbent assay （ELISA）. Local tissues of the points were used to prepare cryosections. The changes of MCs were observed after toluidine blue staining. Results： Compared with group K, the numbers and degranulation rates of MCs in group A, group B, group AB and group CD were significantly increased （all P〈0.05）. The number and degranulation rate of MCs in group AB were significantly higher than those in group A, group B and group CD （all P〈0.05）. The order of histamine levels from high to low was： group AB 〉 group B 〉 group A 〉 group CD 〉 group K, and the differences were statistically significant （all P〈0.05）; the levels of bradykinin in group AB, group A and group B were significantly higher than those in group K and group CD （all P〈O.05）. There was no significant difference in the bradykinin level between group A and group B, nor between group CD and group K （all P〉0.05）. Conclusion： The number and degranulation rate of MCs of the complementary acupionts are significantly increased after acupuncture at complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 9） of rats, and the serum levels of histamine and bradykinin are increased, which may be one of the mechanisms of the synergism produced by the combination of complementary acuDionts.

Effect of electroacupuncture on JAK2/STAT3 pathway in synovial tissues of rats with rheumatoid arthritis

Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There were no significant differences between the EA group and the drug group (all P>0.05). Conclusion: EA can alleviate the inflammatory response of RA rats, improve their pathological conditions, reduce the expressions of phospho-JAK2 and phospho-STAT3 in the synovial tissue of knee joint, and decrease the expressions of JAK2 and STAT3 mRNAs. The therapeutic effect of EA is comparable to that of the tripterygium glycosides. The mechanism of EA treatment may be related to the inactivation of the JAK2/STAT3 pathway.

Effect of electroacupuncture at Lower He-Sea points including Yanglingquan(GB 34)on nuclear factor-κB and interleukin-1βin guinea pigs with acute cholecystitis

Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;macroscopic observation showed that the gallbladder wall was differently thickened;the bile color was dark green and opaque with particles suspended or accumulated;light microscope observation showed that the submucosal blood vessels of the gallbladder were congested,along with mucosal edema,ulceration,necrosis,shedding,and a large number of inflammatory cells infiltrating in the lamina propria,indicating that the AC model was successfully prepared.Compared with the model group,the gallbladder tissue injuries of the four groups receiving EA treatment were all differently repaired,the serum IL-1βlevels were reduced(P<0.01 or P<0.05),and the IL-1βlevels in the gallbladder tissues were reduced(P<0.01 or P<0.05).Compared with the model group,the NF-κB expression level in the Yanglingquan(GB 34)group was significantly reduced(P<0.01),but was not statistical different in the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group(all P>0.05).Compared with the Yanglingquan(GB 34)group,the gallbladder tissues of the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group were more severely damaged,and the expression levels of serum IL-1β,the NF-κB and IL-1βin the gallbladder tissues were increased(P<0.01 or P<0.05).Intervention effect of Yanglingquan(GB 34)on AC guinea pigs was superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).Conclusion:EA at the Lower He-Sea points of the stomach,large intestine,small intestine and gallbladder can produce curative effects on AC guinea pigs and reduced the inflammatory symptoms.Intervention effect of Yanglingquan(GB 34)on AC guinea pigs is superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).The mechanism of EA at Yanglingquan(GB 34)in treating AC may be regulating IL-1βand NF-κB to control the inflammatory response and improve the gallbladder tissue damage.

Instant effect of moxibustion at different points on electrogastrogram of patients with functional dyspepsia

Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.