迈向知识经济时代的隆生——四川隆生集团抓机遇、促发展

四川隆生集团是以饲料生产、农业产业化为主业,集科、工、贸为一体的大中型股份制企业集团。集团下属四川三联饲料有限责任公司、成都龙庆饲料实业有限公司、四川华龙饲料有限责任公司、重庆隆生饲料有限责任公司、四川隆生科技实业发展有限公司、连云港东辛隆生科技饲料有限公司、四川红种子高新农业有限责任公司、成都津鹿包装有限公司等9家核心企业和数十家合作型松散企业。回顾集团诞生以来创业所取得的成就和面对的种种困难,隆生集团的主要做法是:

燕山山脉隆升过程的热年代学分析

本文应用热年代学方法,测定侵入体的热历史,进而分析燕山山脉的隆升过程。研究结果表明,燕山造山带的隆升过程在时空上具有明显非均匀性。燕山中段南部的盘山岩体约于226.48Ma侵位,在96～35Ma期间以3.45℃/Ma的速度冷却,对应于0.115mm/a的快速隆升过程;燕山中段北部的雾灵山岩体约于132Ma侵位,在86～45Ma期间以5.61℃/Ma的速度冷却,对应于0.186mm/a的快速隆升过程;燕山西段的云蒙山岩体约于143Ma侵位,在106～103.95Ma与20～0.0Ma期间分别以31.80℃/Ma、4.45℃/Ma的速度冷却,对应于1.06mm/a、0.15mm/a的快速隆升过程。燕山西段四合堂花岗片麻岩黑云母约于144.46Ma开始启动K-Ar法记年系统,在13Ma以来以7.69℃/Ma的速度冷却,对应于0.256mm/a的快速隆升过程。燕山西南缘的八达岭岩体于129Ma之前侵位,在6Ma以来以16.67℃/Ma的速度快速冷却,对应于0.556mm/a的快速隆升过程。据此笔者认为,燕山中段的快速隆升早于燕山西段,燕山西南缘隆升最晚。

辽西医巫闾山的隆升历史

本文利用裂变径迹年代学方法 ,结合其他同位素年代学资料 ,通过构造热演化的定量分析 ,认为医巫闾山山脉的隆升历史经历了 118～ 81Ma期间的第 1个快速隆升阶段、81～ 14Ma期间的缓慢隆升阶段和 14Ma以来的第 2个快速隆升阶段。医巫闾山的隆升历史与两侧盆地的沉降历史有着很好的对应关系。山脉隆升相对盆地沉降有一个滞后期 ,山脉的快速隆升阶段对应盆地快速沉降的后期和消亡阶段。现今的医巫闾山山脉是 14Ma以来第 2个快速隆升阶段的产物 ,其隆起时间距今大约有 4～ 5Ma。

青藏高原隆升对新生代全球气候变化的影响

青藏高原雄踞亚洲大陆中部，总面积２５０×１０４ｋｍ２，平均海拔４５００ｍ，有“世界屋脊”和“地球第三极”之称。青藏高原隆升是新生代地球地质历史上最重大的事件之一。伴随着距今５０Ｍａ以来青藏高原形成和隆升的历史，不仅改变了高原自身的环境，而且对亚洲甚至...

Effect of Light Intensity and Nutrient Availability on Clonal Growth and Clonal Morphology of the Stoloniferous Herb Halerpestes ruthenica

In a greenhouse experiment plasticity of clonal growth and clonal morphology of the stoloniferous rosette herb Halerpestes nahenica Ovcz. in response to differing levels of light intensity and nutrient availability was studied. Total plant dry weight, leaf area of primary ramets, total number of ramets and of stolons, and total stolon length were significantly reduced, while specific internode length and specific petiole length significantly increased under deep shading (6.25% of high light intensity, 5.3% of full daylight) or under low nutrient availability. Under low nutrient availability, mean stolon internode length of H. ruthenica was significantly larger while branching intensity and number of ramets smaller than those under high nutrient availability. These responses are consistent with the foraging model of clonal plants, indicating that H. nahenica is able to forage nutrients through the plastic responses of clonal growth and clonal. morphology when it grows in heterogeneous environments. However, under deep shading, both mean stolon internode length and mean petiole length were significantly reduced, which disagrees with the findings of many other stoloniferous herbs in response to low or medium levels of shading (ca. 13%-75% of high light intensity, >10% of full daylight), suggesting that under deep shading stoloniferous herbs may not forage light through the plastic responses of spacer length. Many traits such as total plant dry weight, total number and length of stolons, total length of secondary and tertiary stolons. total number of ramets, leaf area of primary ramets and branching intensity were markedly influenced by the interaction effect of light intensity and nutrient availability. Under high light intensity nutrient availability affected these traits more pronouncedly, however under low light intensity nutrient availability either did not affect or affected less markedly on these traits, indicating that fight intensity had significant effect on nutrient foraging in H. nahenica. Under deep shading or low nutrient availability, H. ruthenica may increase its mean stolon internode length by means of thinning stolon internodes (i.e., an increase in specific internode length), which provides it with more chance to escape from resource-poor sites.

Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak

[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.

Rhizome Elongation Growth of Umbrella Bamboo(Fargesia murielae) Seedlings at Its Native Home in Central China

[Objective] The aim was to investigate the rhizome elongation growth of umbrella bamboo （Fargesia murielae） seedlings in China. [Method] The study was conducted in Liangfengya, Shennongjia National Nature Reserve. In the field investigation, six clumps of umbrella bamboo which grow independently were randomly selected and labeled. Rhizome elongation growth parameters, length and diameter of all the ages were measured. The age classes of bamboo rhizome were ascertained by age grade backtracking method. [Result] Field investigation suggested that at seedling phase, rhizome of umbrella bamboo prolonged very quickly under yearly time sequence, following an exponential curve. It indicated that although it was 15 years since the mother population died back, new generation had not been established its stable population yet. [Conclusion] Studying elongation regulation of umbrella bamboo may provide the theory understanding of life cycle of this long lived bamboo species.

Cloning and Bioinformatic Analysis of Cinnamoyl-CoA Reductase Gene (CCR) from Pennisetum purpureum

[Objective] The aim was to clone the cDNA and DNA sequences of the CCR （Cinnamoyl-CoA reductase） gene which involves in lignin biosynthesis, from Pennisetum purpureum, and to make comprehensive analysis on these sequences. [Method] CCR sequences were cloned from P. purpureum by using conventional RT-PCR and RACE （Rapid Amplification of cDNA Ends） methods; and the bioinformatic analyses of the CCR were conducted by means of NCBI, ProtParam ProtScale, TMHMM, TargetP, SignalP, Pfam20.0, Prosite, Swiss-Model, ClustalW2, DNAman, DNAstar and MEGA5. [Result] The cloned PpCCR （P. purpureum CCR） cDNA sequence was 1 316 bp, including a 1 110 bp ORF and 206 bp 3’-UTR. The cloned DNA sequence from PpCCR was 6 133 bp in full-length, containing five exons and four introns. Bioinformatic analysis indicated that PpCCR encoded a polypeptide of 369 amino acids, the secondary structure of which was primarily composed of random coil and α-helix, belonging to NAD-dependent epimerase/dehydratase family, and its co-factor binding sites and substrate binding sites were highly conserved. [Conclusion] DNA and cDNA sequences of CCR gene were obtained from P. purpureum, which had the typical characteristics of other homologous genes. The obtained bioinformatic data provided theoretical references for the further analysis of CCR and better application of P. purpureum in the future.

Bioinformatics Analysis of Chalconesynthase Gene in Morus

[Objective] This study aimed to explore the molecular mechanism of mulberry pigment metabolism regulation. [Method] Chalconesynthase（CHS） gene was cloned from Morus（Moraceae） in silico. The amino acid sequence, physical and chemical properties, transmembrane structural domain, hydrophobicity/hydrophilicity,subcellular localization, secondary and tertiary structure of protein were predicted and analyzed by bioinformatics tools. [Result] The cDNA sequence of CHS gene was 1 365bp by splicing using the software DNAstar and it contained a complete ORF including 1 170 bp which encoded 389 amino acids. Bioinformatic analysis showed that CHS gene included specific peptide sequence RLMMYQQGCFAGGTVLR of chalcone synthase superfamily, but has no signal peptide, belonging to the non-secretory proteins, located inside of cytoplasm. Its molecular evolution is more conservative.[Conclusion] The results above provided foundation for the further studies of structure and function of CHS protein.

Overexpression of Proline Transporter Gene Isolated from Halophyte Confers Salt Tolerance in Arabidopsis

Proline is one of the most important and widespread osmolyte which functions in adaptation to adverse environmental stresses in many organisms. Also it is an important carbon and nitrogen resource in higher plants. Metabolism of proline has been elucidated in many plant species. However, transport of proline was poorly characterized although transport system plays an important role in proline distribution in different tissues. We isolated one full_length cDNA encoding proline transporter from the typical halophyte: Atriplex hortensis L. through cDNA library screening and 5′_RACE. The deduced amino acid sequence had eleven transmembrane domains, showed 60%-69% similarities to other ProTs and the gene was designated AhProT1. In the phylogenetic tree, higher plants' ProTs, e.g. AhProT1, showed more similar to ProP from microorganisms than ProT from mammalians. AhProT1 gene was transformed into Arabidopsis thaliana under 35S promoter. In MS medium containing [U_ 14 C] proline, AhProT1 + plants were able to accumulate much more radiolabeled proline in the roots than control plants. In MS medium containing different concentrations of NaCl, AhProT1 + plants could endure 200 mmol/L NaCl and keep development and biomass increase with proline supply, whereas control plants died back at 150 mmol/L NaCl.

A MONOCLONAL ANTIBODY RECOGNIZING NON DERIVATIVE 13 HYDROXY GIBBERELLINS AND THEIR GLUCOSIDES *

The production and characterization of a monoclonal antibody (MAb AB10) against GA 3 glucoside as well as GA 3 is described. MAb AB10 was derived from an immunogen in which human serum albumin (HSA) was linked to GA 3 at carbon 3. This antibody showed high affinity for GA 3 glucoside as well as for 13 hydroxy gibberellins (GA 1, GA 3, GA 5, etc). The affinity of MAb AB10 for 13 hydroxy GAs was significantly reduced by methylation of the 7 oic acid but not by glycosylation of 3 hydroxyl group. Based on this antibody, both of competitive enzyme linked immunosorbent assays (ELISAs) for GA 3 glucoside and for GA 3 were developed. These two ELISAs displayed linear detection ranges from 0 2 pmol to 20 pmol. Using these assays, the fluctuation of GA 3 like and GA 3 glucoside like substances in the leaves of Rumex japonicus was investigated. The results indicated that the glycosylation of free GAs was connected with leaf senescence and that the function of 6 benzyl amino purine in retarding the leaf senescence was probably related to delaying the process of glycosylation of free GAs.

Cloning, Analysis and Prokaryotic Expression of DsSP Gene from Dunaliella salina

[Objective] The purpose of this study was to clone a starch phosphorylase gene from Dunaliella salina and to preliminarily analyze its basic properties and protein expression. [Method] RT-PCR and RACE （rapid amplification of cDNA ends） method was used for gene cloning; basic properties of the gene were analyzed using bioinformatics method; prokaryotic expression vector PGS21a-DsSP was constructed and transformed into E. coil BL21; the fusion protein was purified and detected by GST-SefinoseTM Kit and Western Blot, respectively. [Result] A starch phos-phorylase gene （GenBank accession No. KF061044） named DsSP was successfully isolated from D. salina. Basic properties, subcellular localization, secondary structure and tertiary structure of the protein were analyzed and predicted. The fusion protein was found in the supernatant and inclusion bodies. The supernatant protein was successfully purified. Western Blot analysis showed that the fusion protein was successfully expressed in E. coil BL21. [Conclusion] This study laid experimental foun- dation for further clarifying the function and mechanism of DsSP.

Isolation of an Arabidopsis Gene Encoding Ins (1,3,4) P_3 5/6-Kinase-like Protein and Involved in Plant Response to Abiotic Stresses

By mRNA differential display from control versus NaCl_shocked Arabidopsis seedlings, we screened an Arabidopsis 3′ partial cDNA, which represents a gene encoding inositol 1,3,4_trisphosphate (Ins(1,3,4)P 3) 5/6_kinase_like protein. Northern blotting analysis showed that the gene, named as AtITL1, is strongly induced by NaCl and low temperature, but not induced by drought and abscisic acid (ABA). Analysis of 5′ region of the AtITL1 found that there are dehydration_responsive element/C_repeat (DRE/CRT) cis _acting elements, but no elements related to G_box and ABRE (ABA_responsive element) in its 5′ region, which is consistent with the expression patterns of the AtITL1 independent of ABA. These results suggest that the AtITL1 may be involved in the osmotic stress response pathway independent of ABA.

Isolation and analysis of pepper ACC oxidase gene partial sequence

Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate （ACC） oxidase （ACO） is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair （bp） candidate fragment was amplified from pepper with primers derived from the ACO sequence （AJ011109） reported by Ernesto. The putative new gene was analyzed by bioinformatics tools.

Primary Observation of the Ecological Habits of Ferirana guadranus in Tianshui City of Gansu Province

The ecological nature of Ferirana guadranus in Liqiao Country of Tianshui City of Guansu Province from May to August in 2010 are primarily investigated.Research found that Ferirana guadranus lives in the mountain streams at an elevation of 1 332 m.The adult frog lives largely above an elevation of 1 320 m,where the water is neutral acid and shady.It belongs to the mountainous stream type.

Cloning and Identification of A New Na^+/H^+ Antiporter Gene ZmSOS1 in Maize(Zea mays L.)

[ Objective] The study aimed to clone and identify Na^＋/H^＋ antiporter genes in maize, and provided the information for characterizing the function of such genes in abiotic stress tolerance of maize. Method The in silico cloning, RT-PCR, and bioinformatics analysis were used in this study. Result By in sifico cloning, a plasma membrane Na^＋/H^＋ antiporter gene, named as ZmSOS1 （EMBL accession No. BN001309）, was cloned from maize （ Zea mays L. ）. ZmSOS1 has an open reading frame （ORF） of 3 411 bp which encoded a protein of 1 136 amino acids. By multiple sequence alignment analysis, it showed the predicated peptide of ZmSOS1 were 61% and 82% identities in amino acids to the plasma membrane Na^＋/H^＋ antiporter AtSOS1 and OsSOS1, respectively. The RT-PCR analysis revealed that ZmSOS1 could be significantly up-regulated by salt stress, which indicated ZmSOS1 might play a role in salt tolerance of maize. Conclusion ZmSOS1 is a putative plasma membrane Na^＋/H^＋ antiporter gene and may play a role in abiotic stress tolerance of maize.

Cloning of the Full-length Gene for Tobacco Ethylene Receptor NTHK2 and Characterization of Its Kinase Domain

Previously the partial sequence of an ethylene receptor gene NTHK2 was isolated from tobacco (Nicotiana tabacum L. var. Xanthi) plants and it was wound and drought inducible. In the present study full-length cDNA of NTHK2 was cloned by 5'-RACE method. NTHK2 gene has 3 216 bp, with 509 bp of 5'-non-coding region and 427 lip of 3'-non-coding region, and encodes an ethylene-receptor homolog of 760, amino acids. NTHK2 protein has a putative signal peptide, three transmembrane domains, a histidine kinase domain and a receiver domain. In the putative histidine kinase domain, the histidine at the phosphorylation site was replaced by an asparagine. To study the biochemical property of NTHK2, its kinase domain was expressed as a fusion protein with glutathione S-transferase (GST) using yeast Schizzosaccharomyces pombe as an expression system. In vitro kinase assay showed that NTHK2 kinase domain can autophosphorylate in the presence of Mg2+, indicating that NTHK2 may function as a kinase. Further studies will elucidate the function of NTHK2 in plant.

Current Status of Rice Production in Burundi and Its Development Strategies

Burundi, a landlocked country locked near the equator, is one of the least developed countries confirmed by the United Nations and the 60% financial revenue needs aid. Rice is the most important and extravagant food in Burundian consumption for no more than 5 kg on average per people by year. It＇s especially suitable to plant rice in Burundi because of the sufficient sunlight and rain. But the lack of new varieties, practical technology and modern service make it very difficult for rice production. According to this significant roll, the rice production has to be increased in order to satisfy the consumption needs which are now becoming higher and keep the food safe. This article mainly shows the rice ecological types, production facts and the major constraints of rice production in Burundi, and then look into the distance on how to solve the problems.

Cloning of Rabbit Bone Morphogenetic Protein 15 and Its Expression During in vitro Maturation of Rabbit Oocytes

Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%-90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation （IVM） was measured by fluorescent quantitative RT-PCR method. BMP 15 was expressed at low levels in immature oocytes and increased to the highest level at 16h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP 15 suggested that it might be important in cumulus expansion in rabbits.