模拟精子与卵细胞随机结合的实验教具

通过自制模拟精子与卵细胞随机结合的实验装置教具,让学生快速理解生男生女的机会是均等的。

重链和轻链随机结合后抗D抗体亲合力的调整

背景 抗体的的重链在确定抗原的特异性上比轻链更加重要,这一点通常被人们接受。在抗D抗体中,H链在接合抗原的突出作用已被广泛认识,但是L链的功能还没有被充分认识。在本项研究中,L链的作用通过随机结合L链进行研究,以确定来自非D免疫供者的抗D抗体的特异性和反应性。研究设计和方法 在噬菌体载体系统中(pComb3H,Scripps Institute)用43F10抗D抗体H链结合L链文库中不同k型轻链。

重链与轻链随机结合后抗D亲合力的调变

背景 一般认为抗体重链(H)在决定抗原的特异性方面比轻链更重要。抗-D抗体H链在结合抗原的重要作用已被充分认识,但对L链功能的认识却很少。在这项研究中,通过除去L链以研究非D免疫供者抗-D抗体L链在决定抗体的特异性和反应性方面的功能。研究设计和方法

模拟精子与卵细胞随机结合的创新实验教具

在生物学教学中,模拟实验可以使学生的感官全面开发,赋予学生积极、主动的学习动力,让学生在情境模拟中真正感悟生物学原理。人教版八年级下册生物学“人的性别遗传”中设计了“模拟精子与卵细胞随机结合”的实验。通过教师引导学生自制创新实验教具来模拟精子与卵细胞的随机结合,分析并帮助学生在自制实验教具的过程中,以实践创新摆脱思维的束缚,让学生更直观地理解精子与卵细胞随机结合这一抽象知识以及生男生女机会均等这一概念。

运用Excel 2010模拟“精子和卵细胞随机结合”的实验

笔者尝试运用Excel 2010模拟人教版义务教育《生物学》八年级下册第2章第4节"人的性别遗传"中"精子和卵细胞随机结合"的实验内容。体现了信息技术与生物学的融合,有利于学生加深对重要概念的理解。

随机提问与小学语文课堂的有效结合

在小学阶段的学习过程中,因为学生在课堂中没有太多的学习任务,教师在讲解完知识内容之后,基本上也不会为学生布置太多的课后作业,所以学生在课下的业余时间是非常多的,但是因为小学生并没有什么时间观念,所以对于课余时间的利用效率是非常的低下的,学生在课堂的学习过程中也没有充足的学习动力,在课下的时间又没有及时的对知识内容进行复习,因此,本文就随机提问与小学语文课堂的有效结合进行探讨,并提出建议。

Characterization of the dissociation pathways of dichloromethane and glutathione in dichloromethane dehalogenase

Dichloromethane(DCM)dehalogenase stands as a crucial enzyme implicated in the degradation of methylene chloride across diverse environmental and biological contexts.However,the unbinding pathways of ligands from DCM dehalogenase remain unexplored.In order to gain a deeper understanding of the binding sites and dissociation pathways of dichloromethane(DCM)and glutathione(GSH)from the DCM dehalogenase,random accelerated molecular dynamics(RAMD)simulations were performed,in which DCM and GSH were forced to leave the active site.The protein structure was predicted using Alphafold2,and the conformations of GSH and DCM in the binding pocket were predicted by docking.A long equilibrium simulation was conducted to validate the structure of the complex.The results show that GSH is most commonly observed in three main pathways,one of which is more important than the other two.In addition,DCM was observed to escape along a unique pathway.The key residues and protein helices of each pathway were identified.The results can provide a theoretical foundation for the subsequent dissociation mechanism of DCM dehalogenase.

探究物理模型在“减数分裂与受精作用”中的应用

“减数分裂与受精作用”是高中生物学科的重点和难点之一,此部分内容较抽象,适合用较直观的物理模型进行教学。本文主要以减数分裂的过程为基础,用图画描述了非同源染色体的自由组合、同源染色体上非姐妹染色单体的交叉互换、受精时精子和卵细胞的随机结合对有性生殖后代多样性的影响。

STUDIES ON THERMODYNAMICAL PROPERTIES OF RANDOM-BOND ISING MODEL IN A TRANSVERSE FIELD WITH CEA AND DPIR

A effective approximate scheme which is combined by cluster with the discrelized path-integral representation (DPIR) is used in the study on the random-bond Ising model in a transverse field (RTIM). The critical thermodynamical properties, such as the critical temperature, the critical transverse field, the average magnetization ,the susceptibility and the special heat atc.. are calculated, And some results have been improved.

Efficacy and safety of electroacupuncture for perimenopausal insomnia: a randomized controlled trial

Objective: To observe the effectiveness and safety of electroacupuncture (EA) plus Luohua Anshen oral liquid for patients with perimenopausal insomnia. Methods: A total of 66 participants who met the inclusion criteria were enrolled in the randomized controlled trial and allocated to a treatment group and a control group at a ratio of 1:1, with 33 cases in each group. Both groups were given Luohua Anshen oral liquid as a basic treatment. The treatment group was additionally given EA every other day, three times a week. Both groups were treated for four weeks and a four-week follow-up was conducted. The scores of Pittsburgh sleep quality index (PSQI), Kupperman index (KI) and traditional Chinese medicine sleep syndrome scale (TCMSSS) were recorded at pre- and post-treatment, and at the follow-up. Meanwhile, adverse effects were monitored and recorded. Results: After four-week treatment, the global scores of PSQI, KI and TCMSSS in both groups declined significantly (all P<0.05), and the decreases in the treatment group were more significant than those in the control group (allP<0.05). The global scores of PSQI, KI and TCMSSS in both groups at the follow-up visit were significantly different from the corresponding baseline (allP<0.05), while insignificantly different from those assessed at post-treatment (allP>0.05). The total effective rate was 93.9% in the treatment group, significantly higher than 72.2% in the control group (P<0.05). No significant adverse event was reported in this trial excepted one patient experienced slight dizziness in the first acupuncture treatment. Conclusion: EA plus Luohua Anshen oral liquid is safe for perimenopausal insomnia with satisfactory short- and long-term effectiveness, and it shows certain advantage compared with using Luohua Anshen oral liquid alone.

Structure condition under initial enlargement of filtration

We address the question of how the structure condition is affected when one possesses some additional information at the very beginning of the investment period.The structure condition represents essentially an alternative to non-arbitrage conditions for the Markowitz’s portfolio optimization framework,and is crucial for the existence of the optimal portfolio in quadratic utility settings.Herein,we provide practical assumption on the initial market model and the additional information to preserve the structure condition.The stochastic tools that drive this result are a generalization of the Lazaro-Yor representation by Lazaro and Yor(1978)and optional stochastic integral.

Integrated traditional and Western medicine for treatment of depression based on syndrome differentiation:a meta-analysis of randomized controlled trials based on the Hamilton depression scale

OBJECTIVE:To systematically review the benefits of integrated traditional and Western medicine therapies based on the Hamilton depression scale(HAMD) following syndrome differentiation of depression.METHODS:We searched six English and Chinese electronic databases for randomized clinical trials(RCTs) on integrated traditional and Western medicine for treatment of depression.Two authors extracted data and independently assessed the trial quality.RevMan 5 software was used for data analyses with an effect estimate presented as weighted mean difference(WMD) with a 95% confidence interval(CI).RESULTS:Seven RCTs with 576 participants were identified for this review.All trials were eligible for the meta-analysis and were evaluated as unclear or having a risk of bias.Meta-analysis showed,compared with Western medicine alone,integrated traditional and Western medicine based on syndrome differentiation could improve the effect of treatment represented by the HAMD [WMD=-2.39,CI(-2.96,-1.83),Z=8.29,P<0.00001].There were no reported serious adverse effects that were related to integrated traditional and Western medicine based therapies in these trials.CONCLUSIONS:Integrated traditional and Western medicine based therapies for the syndrome differentiation of depression significantly improved the HAMD,illustrating that combining therapies from integrated traditional and Western medicine for treatment of depression is better than Western medicine alone.However,further large,rigorously designed trials are warranted due to the insufficient methodological rigor seen in the trials included in this study.

Fault feature enhancement of gearbox in combined machining center by using adaptive cascade stochastic resonance

The difficulty to select the best system parameters restricts the engineering application of stochastic resonance （SR）. An adaptive cascade stochastic resonance （ACSR） is proposed in the present study. The proposed method introduces correlation theory into SR, and uses correlation coefficient of the input signals and noise as a weight to construct the weighted signal-to-noise ratio （WSNR） index. The influence of high frequency noise is alleviated and the signal-to-noise ratio index used in traditional SR is improved accordingly. The ACSR with WSNR can obtain optimal parameters adaptively. And it is not necessary to predict the exact frequency of the target signal. In addition, through the secondary utilization of noise, ACSR makes the signal output waveforrn smoother and the fluctuation period more obvious. Simulation example and engineering application of gearbox fault diagnosis demonstrate the effectiveness and feasibility of the proposed method.

Numerical study of soil-rock mixture:Generation of random aggregate structure

The soil-rock mixture(SRM) is highly heterogeneous. Before carrying out numerical analysis,a structure model should be generated. A reliable way to obtain such structure is by generating random aggregate structure based on random sequential addition(RSA). The classical RSA is neither efficient nor robust since valid positions to place new inclusions are formulated by trial, which involves repetitive overlapping tests. In this paper, the algorithm of Entrance block between block A and B(EAB)is synergized with background mesh to redesign RSA so that permissible positions to place new inclusions can be predicted,resulting in dramatic improvement in efficiency and robustness.

Band Alignment for Ambipolar-Doping of Sn_xZn_(1-x) Te Alloys

Using the first-principles band-structure method and a special quasirandom structure(SQS) approach,we have systematically calculated the alloy bowing coefficients and the nature band offsets of SnxZn1-x Te alloys.We show that the bowing coefficients and band gaps of these alloys are sensitively composition dependent.Due to wave functions full overlapping and delocalization of the Sn outermost p orbits and Zn s orbits,the coupling between these states is very strong,resulting in a significant downshift of conduction band edge with the increase of the Sn concentration x,While the valence band edge keeps almost unchanged compared with that of the binary ZnTe,thus improving the possibility for ambipolar-doping.

Effect of Jianpi therapy in treatment of chronic obstructive pulmonary disease:a systematic review

OBJECTIVE： To evaluate the efficacy of Jianpi thera- py in Traditional Chinese Medicine （TCM） for treat- ment of chronic obstructive pulmonary disease （COPD） in stable phase by performing a systematic review and meta-analysis. METHODS： The literatures concerning randomized controlled trials （RCTs） and quasi-RCTs comparing TCM treatment plus Western Medicine （WM） treat- ment with TCM alone, orTCM treatment vs no treatment, placebo for stable phase of COPD were searched in PubMed （1990-December 2010）, in Eng- lish and using Chinese National Knowledge Infra- structure （CNKI, 1990-December 2010）, Chinese Bio- medical Database （1990-December 2010）, Wang- fang Database （1990-December 2010）, and Weipu （VlP） Database in Chinese. The quality assessment and data extraction for RCTs were conducted by two reviewers independently. Jadad scale and allo-cation concealment were used to assess the quality of the included studies, and meta-analyses were conducted with the Collaboration＇s Revman 5.0 software. RESULTS： Seventeen RCTs or quasi-RCTs involving 1269 patients were included. The methodological quality was poor in all trials except one trial （Jadad score=4）. In the meta-analysis, TCM-WM treatment was significantly superior to WM treatment in cure rate [0R=3.82, 95%C/（2.45, 5.95）], and the effective rate between TCM treatment and placebo also had significant difference [0R=4.31, 95%C/（2.35, 7.91）]. Moreover, pulmonary function of the patients in TCM-WM group and TCM group was significantly improved [forced vital capacity （FVC）, P=O.01, quali- ty of life, P〈O.O01 ]. CONCLUSION： The experience in TCM-WM treat- ment of COPD in stable phase was encouraging. The current evidence shows that TCM-WM treat- ment might be more efficient in effective rate, qual- ity of life, and FVC than WM treatment alone. But for forced expiratory volume in one second （FEVl） and FEV1/FVC, no matter TCM-WM treatment com- pared with WM treatment alone or TCM treatment compared with placebo, there was no significant difference, with no obvious adverse reactions. Due to the low methodological quality of trials includ- ed, more RCTs of high quality in large scale are re- quired.

AN IMPROVED RANDOM WALK SEGMENTATION ON THE LUNG NODULES

In this paper, we proposed a semi-automatic technique with a marker indicating the target to locate and segment nodules. For the lung nodule detection, we develop a Gabor texture feature by FCM （Fuzzy C Means） segmentation. Given a marker indicating a rough location of the nodules, a decision process is followed by applying an ellipse fitting algorithm. From the ellipse mask, the foreground and background seeds for the random walk segmentation can be automatically obtained. Finally, the edge of the nodules is obtained by the random walk algorithm. The feasibility and effectiveness of the proposed method are evaluated with the various types of the nodules to identify the edges, so that it can be used to locate the nodule edge and its growth rate.