The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medi...The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medium was obtained (g/L):Glucose,65;Yeast extract,2.0;KNO 3 3.0,KH 2PO 4 1.0,MgSO 4·7H 2O 0.6,NaCl 5.8,CaCl 2·2H 2O 0.1,ZnSO 4·7H 2O 0.0067,FeCl 3·6H 2O 0.014,CuSO 4·5H 2O 0.0004,MnSO 4·H 2O 0.0002.Under the optimum culture conditions,the microalgae dry cell weight and eicosapentaenoic acid was 18.3g/L and 0.891 g/L,respectively.The submersion culture process was analysed.展开更多
文摘为了得到寇氏隐甲藻突变株的最佳发酵条件。先通过单因素试验考察接种量、培养时间、培养温度、装液量、培养基初始p H值等因素对二十二碳六烯酸产量的影响,在此基础上选择接种量、培养基初始p H、装液量为主要影响因素,通过Box-Behnken设计,得到最优发酵条件为接种量9.2%,初始p H值为6.9,装液量为65 m L/500 m L时,DHA产量最大为5.81 g/L,优化后DHA的产量比优化前提高了13.28%。
文摘The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medium was obtained (g/L):Glucose,65;Yeast extract,2.0;KNO 3 3.0,KH 2PO 4 1.0,MgSO 4·7H 2O 0.6,NaCl 5.8,CaCl 2·2H 2O 0.1,ZnSO 4·7H 2O 0.0067,FeCl 3·6H 2O 0.014,CuSO 4·5H 2O 0.0004,MnSO 4·H 2O 0.0002.Under the optimum culture conditions,the microalgae dry cell weight and eicosapentaenoic acid was 18.3g/L and 0.891 g/L,respectively.The submersion culture process was analysed.
