Objective To observe the clinical efficacy on simple obesity of spleen and kidney yang deficiency pattern/syndrome in females treated with electroacupuncture and isolated-medicinal moxibustion.Methods Fifty-eight case...Objective To observe the clinical efficacy on simple obesity of spleen and kidney yang deficiency pattern/syndrome in females treated with electroacupuncture and isolated-medicinal moxibustion.Methods Fifty-eight cases were randomized into an acupuncture-moxibustion group(28 cases) and an electroacupuncture group(30 cases).In the electroacupuncture(EA) group,EA was applied to Zhōngwǎn(中脘 CV 12),Xiàwǎn(下脘 CV 10),Qìhǎi(气海 CV 6),Guānyuán(关元 CV 4),Tiānshū(天枢 ST 25),Dàhéng(大横 SP 15),Jiǎnféidiǎn(减肥点 Extra),Ashi(阿是穴) and others,in which Tiānshū(天枢 ST 25) made a pair with Dàhéng(大横 SP 15) for electric stimulation,Jiǎnféidiǎn(减肥点 Extra) made the pairs with Xiàwǎn(下脘 CV 10) and Qìhǎi(气海 CV 6) separately for electric stimulation.The needles were remained for 40 min.In the acupuncture-moxibustion(AM) group,on the basis of the treatment as the EA group,the isolated-medicinal moxibustion was supplemented at Píshū(脾俞 BL 20) and Shènshū(肾俞 BL 23).Five treatments made one session.The efficacy was assessed after 3 sessions.Results The total effective rate was 92.9%(26/28) in the AM group,which was superior to 70.0%(21/30) in the EA group(P〈0.05).Conclusion Electroacupuncture in combination with isolated-medicinal moxibustion improves the efficacy on simple obesity of spleen and kidney yang deficiency pattern/syndrome in females,which is superior to the efficacy with simple electroacupuncture therapy.展开更多
Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibus...Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibustion intervening CACC through the purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)pathway.Methods:A total of 26 male Sprague-Dawley rats were selected.According to the random number table method,6 rats were selected as the normal group.The remaining 20 rats were injected intraperitoneally with azoxymethane(AOM)combined with oral dextran sodium sulfate(DSS)to prepare the CACC model.After the model was successfully established,2 rats were randomly selected for model identification.The remaining 18 rats which were successfully modeled were randomly divided into a model group,a herb-partitioned moxibustion group and a ginger-partitioned moxibustion group,with 6 rats in each group.Moxibustion intervention was performed in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group at Qihai(CV 6)and bilateral Tianshu(ST 25).Moxibustion was performed twice at each point each time,once a day,at a 1-day interval after 6 consecutive interventions,for a total of 30 interventions.After intervention,the colon tumor load,pathological change and histopathological score were observed.Immunohistochemistry was used to detect the expressions of VEGF,P2X7R,phospho-STAT3(p-STAT3),and nuclear factor-kappa B p65(NF-κB p65)proteins in rat colon tissue.Western blot was used to detect the levels of p-STAT3 and NF-κB p65 proteins in rat colon tissue.Results:Compared with the normal group,the colon tumor load and histopathological score in the model group were significantly increased(both P<0.001),and different grades of dysplasia were observed in colon tissue from the model group,reaching the degree of adenocarcinoma;the expression level of P2X7R protein in colon tissue was significantly decreased(P<0.001),and the expression levels of p-STAT3,NF-κB p65 and VEGF proteins were significantly increased(all P<0.001)in the model group.Compared with the model group,the colon tumor load,colon histopathological score and the levels of p-STAT3,NF-κB p65 and VEGF proteins in colon tissue were significantly decreased(all P<0.05)in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group while the expression levels of P2X7R protein in colon tissue were significantly increased(both P<0.05).Conclusion:Both herb-partitioned moxibustion and ginger-partitioned moxibustion can reduce the colon tumor load in CACC rats and delay the progression of colon adenomas.The mechanism may be mediated by the P2X7R/STAT3 pathway to inhibit STAT3 phosphorylation,thereby reducing VEGF protein expression.展开更多
文摘Objective To observe the clinical efficacy on simple obesity of spleen and kidney yang deficiency pattern/syndrome in females treated with electroacupuncture and isolated-medicinal moxibustion.Methods Fifty-eight cases were randomized into an acupuncture-moxibustion group(28 cases) and an electroacupuncture group(30 cases).In the electroacupuncture(EA) group,EA was applied to Zhōngwǎn(中脘 CV 12),Xiàwǎn(下脘 CV 10),Qìhǎi(气海 CV 6),Guānyuán(关元 CV 4),Tiānshū(天枢 ST 25),Dàhéng(大横 SP 15),Jiǎnféidiǎn(减肥点 Extra),Ashi(阿是穴) and others,in which Tiānshū(天枢 ST 25) made a pair with Dàhéng(大横 SP 15) for electric stimulation,Jiǎnféidiǎn(减肥点 Extra) made the pairs with Xiàwǎn(下脘 CV 10) and Qìhǎi(气海 CV 6) separately for electric stimulation.The needles were remained for 40 min.In the acupuncture-moxibustion(AM) group,on the basis of the treatment as the EA group,the isolated-medicinal moxibustion was supplemented at Píshū(脾俞 BL 20) and Shènshū(肾俞 BL 23).Five treatments made one session.The efficacy was assessed after 3 sessions.Results The total effective rate was 92.9%(26/28) in the AM group,which was superior to 70.0%(21/30) in the EA group(P〈0.05).Conclusion Electroacupuncture in combination with isolated-medicinal moxibustion improves the efficacy on simple obesity of spleen and kidney yang deficiency pattern/syndrome in females,which is superior to the efficacy with simple electroacupuncture therapy.
文摘Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibustion intervening CACC through the purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)pathway.Methods:A total of 26 male Sprague-Dawley rats were selected.According to the random number table method,6 rats were selected as the normal group.The remaining 20 rats were injected intraperitoneally with azoxymethane(AOM)combined with oral dextran sodium sulfate(DSS)to prepare the CACC model.After the model was successfully established,2 rats were randomly selected for model identification.The remaining 18 rats which were successfully modeled were randomly divided into a model group,a herb-partitioned moxibustion group and a ginger-partitioned moxibustion group,with 6 rats in each group.Moxibustion intervention was performed in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group at Qihai(CV 6)and bilateral Tianshu(ST 25).Moxibustion was performed twice at each point each time,once a day,at a 1-day interval after 6 consecutive interventions,for a total of 30 interventions.After intervention,the colon tumor load,pathological change and histopathological score were observed.Immunohistochemistry was used to detect the expressions of VEGF,P2X7R,phospho-STAT3(p-STAT3),and nuclear factor-kappa B p65(NF-κB p65)proteins in rat colon tissue.Western blot was used to detect the levels of p-STAT3 and NF-κB p65 proteins in rat colon tissue.Results:Compared with the normal group,the colon tumor load and histopathological score in the model group were significantly increased(both P<0.001),and different grades of dysplasia were observed in colon tissue from the model group,reaching the degree of adenocarcinoma;the expression level of P2X7R protein in colon tissue was significantly decreased(P<0.001),and the expression levels of p-STAT3,NF-κB p65 and VEGF proteins were significantly increased(all P<0.001)in the model group.Compared with the model group,the colon tumor load,colon histopathological score and the levels of p-STAT3,NF-κB p65 and VEGF proteins in colon tissue were significantly decreased(all P<0.05)in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group while the expression levels of P2X7R protein in colon tissue were significantly increased(both P<0.05).Conclusion:Both herb-partitioned moxibustion and ginger-partitioned moxibustion can reduce the colon tumor load in CACC rats and delay the progression of colon adenomas.The mechanism may be mediated by the P2X7R/STAT3 pathway to inhibit STAT3 phosphorylation,thereby reducing VEGF protein expression.