The primer pair sex1/sex2,which can be widely applied for sex identification in Galliform species,was used to determine the sex of 17 Passeriform species.As CHD-W fragments tended to be preferentially amplified,which ...The primer pair sex1/sex2,which can be widely applied for sex identification in Galliform species,was used to determine the sex of 17 Passeriform species.As CHD-W fragments tended to be preferentially amplified,which may cause unnecessary misidentification in bird species with little difference between CHD-Z and CHD-W,we modified sex1 and sex2,obtaining sex1' and sex-mix respectively.Primer sets were then recombined to conduct sex identification.After testing several Passeriforme birds of known sex,we found that the primer pair sex1'/sex2 was better at limiting the preferential amplification of CHD-W fragments.As they are being frequently used in sex allocation study of Aegithalos concinnus and song learning research of Lonchura striata,we can expect more applications of this primer pair to further studies in Passeriformes.展开更多
The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogen...The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogenetic relationships of 18 species of passerines were studied using Adenylate Kinase lntron 5 （AKS） sequences and DNA techniques. Through sequences analysis in comparison with each other, phylogenetic tree figures of 18 species of passerines were constructed using Neighbor-Joining （N J） and Maximum-Parsimony （MP） meth- ods . The results showed that sylviids should be listed as an independent family, while robins and flycatchers should be listed into Musci- capidae. Since the phylogenetic relationships between long-tailed tits and old world warblers are closer than that between long-tailed tits and parids, the long-tailed tits should be independent of paridae and be categorized into aegithalidae. Muscicapidae and Paridae are known to be two monophylitic families, but Sylviidae is not a monophyletic group. AK5 sequences had better efficacy in resolving close relationships of interspecies among intrageneric groups.展开更多
基金supported by the National Natural Science Foundation of China (Nos.30570234,30330050)
文摘The primer pair sex1/sex2,which can be widely applied for sex identification in Galliform species,was used to determine the sex of 17 Passeriform species.As CHD-W fragments tended to be preferentially amplified,which may cause unnecessary misidentification in bird species with little difference between CHD-Z and CHD-W,we modified sex1 and sex2,obtaining sex1' and sex-mix respectively.Primer sets were then recombined to conduct sex identification.After testing several Passeriforme birds of known sex,we found that the primer pair sex1'/sex2 was better at limiting the preferential amplification of CHD-W fragments.As they are being frequently used in sex allocation study of Aegithalos concinnus and song learning research of Lonchura striata,we can expect more applications of this primer pair to further studies in Passeriformes.
文摘The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogenetic relationships of 18 species of passerines were studied using Adenylate Kinase lntron 5 （AKS） sequences and DNA techniques. Through sequences analysis in comparison with each other, phylogenetic tree figures of 18 species of passerines were constructed using Neighbor-Joining （N J） and Maximum-Parsimony （MP） meth- ods . The results showed that sylviids should be listed as an independent family, while robins and flycatchers should be listed into Musci- capidae. Since the phylogenetic relationships between long-tailed tits and old world warblers are closer than that between long-tailed tits and parids, the long-tailed tits should be independent of paridae and be categorized into aegithalidae. Muscicapidae and Paridae are known to be two monophylitic families, but Sylviidae is not a monophyletic group. AK5 sequences had better efficacy in resolving close relationships of interspecies among intrageneric groups.
