Understanding basic reproductive physiology is crucial for the management of both captive and free-ranging wolf. In the present study, we determined hormonal changes during pregnancy and the estrous cycle in captive f...Understanding basic reproductive physiology is crucial for the management of both captive and free-ranging wolf. In the present study, we determined hormonal changes during pregnancy and the estrous cycle in captive female wolf by measuring fecal steroids collected during the breeding season with high performance liquid chromatography （HPLC）. These biochemical analyses were validated using chemical devivatization and mass spectrometry, and interpreted along with the behavioral data. All four females undergoing estrus cycles were copulated with their partners and delivered pups successfully. We found that estradiol concen-trations were significantly higher during the estrus cycle than other stages （p0.01） and progesterone was also significantly increased throughout the pregnancy （p0.01）. These hormonal fluctuations demonstrated pregnancy-specific changes in the fecal progesterone and estradiol con-centrations. Patterns of fecal estradiol and progesterone concentrations during estrous cycles were similar to those reported for other canids.展开更多
目的利用计算机虚拟筛选技术对芹菜素的潜在靶标进行预测。方法采用PharmMapper网络服务器进行初步预测,利用PyRx0.8中的Autodock Vina模块进行分子对接,并结合文献挖掘进行验证,利用Discovery Studio 3.5对靶蛋白-小分子复合物的相互...目的利用计算机虚拟筛选技术对芹菜素的潜在靶标进行预测。方法采用PharmMapper网络服务器进行初步预测,利用PyRx0.8中的Autodock Vina模块进行分子对接,并结合文献挖掘进行验证,利用Discovery Studio 3.5对靶蛋白-小分子复合物的相互作用模式进行分析。结果 PharmMapper筛选显示芹菜素与胰岛素受体、17β-雌二醇脱氢酶1、组织蛋白酶K的结合较好;结合文献挖掘结果,发现胰岛素受体有相关实验研究,而其他2种则少见报道;利用分子对接技术分析芹菜素与靶蛋白核心氨基酸具有氢键、范德华力、静电作用力等相互作用。结论芹菜素的潜在靶标最有可能是胰岛素受体,而17β-雌二醇脱氢酶1和组织蛋白酶K也存在可能性。展开更多
Steroid sex hormones, such as estradiol-17β （E2） and testosterone （T）, are important regulators of sex change in fish. In this study, we examined the effects of E2 treatment on the dynamics of E2 and T during gon...Steroid sex hormones, such as estradiol-17β （E2） and testosterone （T）, are important regulators of sex change in fish. In this study, we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichtbys olivaceus using histology and radioimmunoassay （RIA）. Flounder larvae were divided into five groups （G0~G4）, and fed with 0 （control）, 0.2, 2, 20 and 100 mg E2/kg feed from 35 to 110 day post hatching （dph）. Fish growth in the G1 and G2 groups was not significantly different from that of the control group （P〉0.05）, while fish in the G3 and G4 groups were less active and showed growth depression and high mortality. The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue. The frequency of females in the G0-G4 groups was 54.5%, 75.0%, 100%, 100% and 93.3%, respectively. The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation, and increased slightly at the onset of ovarian differentiation, while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group. In the E2-treated groups, T levels decreased before the onset of ovarian differentiation. E2 levels were high on the 48 dph, but declined to a lower level on the 54 dph, and then increased gradually during gonadal differentiation. And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation. The data suggest that T and E2 play important roles during gonadal differentiation, and an E2 dose of 2 mg/kg feed could induce sex reversal in P olivaceus.展开更多
Steroid hormones participate in the modulation of serotonergic transmission, including the regulation of synthetic and metabolic enzyme production, as well as receptor and transporter activity. The changes of 5-HT5A a...Steroid hormones participate in the modulation of serotonergic transmission, including the regulation of synthetic and metabolic enzyme production, as well as receptor and transporter activity. The changes of 5-HT5A and 5-HT2c immunolabeling induced by steroids in the hippocampus ofovariectomized rats were studied in this work. Densitometric analysis in rat hippocampi were carried out for adjacent brain coronal immunolabeled sections after treatment with subcutaneous injections of vehicle, estradiol, progesterone or the combination of both steroids in ovariectomized rats. Exposure to estradiol and the combination of estradiol and progesterone significantly reduced the 5-HT5A-like immunosignal in the CA 1 region while progesterone did not induce changes. On the other hand, exposure to the combination of estradiol and progesterone or estradiol alone increased the 5-HT2c immunosignal in the same region. These results indicate that estradiol is involved in the discrete regulation of serotonin receptors 5-HT5A and 5-HT2c in rat hippocampus.展开更多
Objective：To observe the effect of acupuncture for infertility due to luteal phase defect （LPD）.Method：Fifty cases were randomized into an acupuncture group （30 cases） and a medication （Clomiphene） group （20 ...Objective：To observe the effect of acupuncture for infertility due to luteal phase defect （LPD）.Method：Fifty cases were randomized into an acupuncture group （30 cases） and a medication （Clomiphene） group （20 cases）.High phase score （HPS） was adopted to evaluate the luteal function before and after treatments,coupled with observation on the changes of E 2,progesterone （P）,follicles and endometrium.Results：The total effective rate in the acupuncture group was 93.4%,versus 70.0% in the medication group,showing a significant difference （P〈0.05）.Conclusion：Both acupuncture and Clomiphene can substantially improve the luteal function;however,acupuncture can get a better effect in improving the endometrium and a lower miscarriage rate than Clomiphene.展开更多
基金This research was supported by Program for New Century Excellent Talents (NCET-07-0507)Shandong Province Natural Science Foundation (Z2008D01)+1 种基金Project of Science and Technology Development Plan in Shandong Province (2007GG2009011)Shandong Province Science Fund for Distinguished Young Scholars(2005BS02005, 2008BS09011)
文摘Understanding basic reproductive physiology is crucial for the management of both captive and free-ranging wolf. In the present study, we determined hormonal changes during pregnancy and the estrous cycle in captive female wolf by measuring fecal steroids collected during the breeding season with high performance liquid chromatography （HPLC）. These biochemical analyses were validated using chemical devivatization and mass spectrometry, and interpreted along with the behavioral data. All four females undergoing estrus cycles were copulated with their partners and delivered pups successfully. We found that estradiol concen-trations were significantly higher during the estrus cycle than other stages （p0.01） and progesterone was also significantly increased throughout the pregnancy （p0.01）. These hormonal fluctuations demonstrated pregnancy-specific changes in the fecal progesterone and estradiol con-centrations. Patterns of fecal estradiol and progesterone concentrations during estrous cycles were similar to those reported for other canids.
文摘目的利用计算机虚拟筛选技术对芹菜素的潜在靶标进行预测。方法采用PharmMapper网络服务器进行初步预测,利用PyRx0.8中的Autodock Vina模块进行分子对接,并结合文献挖掘进行验证,利用Discovery Studio 3.5对靶蛋白-小分子复合物的相互作用模式进行分析。结果 PharmMapper筛选显示芹菜素与胰岛素受体、17β-雌二醇脱氢酶1、组织蛋白酶K的结合较好;结合文献挖掘结果,发现胰岛素受体有相关实验研究,而其他2种则少见报道;利用分子对接技术分析芹菜素与靶蛋白核心氨基酸具有氢键、范德华力、静电作用力等相互作用。结论芹菜素的潜在靶标最有可能是胰岛素受体,而17β-雌二醇脱氢酶1和组织蛋白酶K也存在可能性。
基金Supported by the National Natural Science Foundation of China(No.30571445)the National High Technology Research and Development Program of China(863 Program)(No.2006AA10A404)the Earmarked Fund for Modern Agro-Industry Technology Research System,China(No.nycytx-50)
文摘Steroid sex hormones, such as estradiol-17β （E2） and testosterone （T）, are important regulators of sex change in fish. In this study, we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichtbys olivaceus using histology and radioimmunoassay （RIA）. Flounder larvae were divided into five groups （G0~G4）, and fed with 0 （control）, 0.2, 2, 20 and 100 mg E2/kg feed from 35 to 110 day post hatching （dph）. Fish growth in the G1 and G2 groups was not significantly different from that of the control group （P〉0.05）, while fish in the G3 and G4 groups were less active and showed growth depression and high mortality. The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue. The frequency of females in the G0-G4 groups was 54.5%, 75.0%, 100%, 100% and 93.3%, respectively. The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation, and increased slightly at the onset of ovarian differentiation, while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group. In the E2-treated groups, T levels decreased before the onset of ovarian differentiation. E2 levels were high on the 48 dph, but declined to a lower level on the 54 dph, and then increased gradually during gonadal differentiation. And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation. The data suggest that T and E2 play important roles during gonadal differentiation, and an E2 dose of 2 mg/kg feed could induce sex reversal in P olivaceus.
文摘Steroid hormones participate in the modulation of serotonergic transmission, including the regulation of synthetic and metabolic enzyme production, as well as receptor and transporter activity. The changes of 5-HT5A and 5-HT2c immunolabeling induced by steroids in the hippocampus ofovariectomized rats were studied in this work. Densitometric analysis in rat hippocampi were carried out for adjacent brain coronal immunolabeled sections after treatment with subcutaneous injections of vehicle, estradiol, progesterone or the combination of both steroids in ovariectomized rats. Exposure to estradiol and the combination of estradiol and progesterone significantly reduced the 5-HT5A-like immunosignal in the CA 1 region while progesterone did not induce changes. On the other hand, exposure to the combination of estradiol and progesterone or estradiol alone increased the 5-HT2c immunosignal in the same region. These results indicate that estradiol is involved in the discrete regulation of serotonin receptors 5-HT5A and 5-HT2c in rat hippocampus.
文摘Objective：To observe the effect of acupuncture for infertility due to luteal phase defect （LPD）.Method：Fifty cases were randomized into an acupuncture group （30 cases） and a medication （Clomiphene） group （20 cases）.High phase score （HPS） was adopted to evaluate the luteal function before and after treatments,coupled with observation on the changes of E 2,progesterone （P）,follicles and endometrium.Results：The total effective rate in the acupuncture group was 93.4%,versus 70.0% in the medication group,showing a significant difference （P〈0.05）.Conclusion：Both acupuncture and Clomiphene can substantially improve the luteal function;however,acupuncture can get a better effect in improving the endometrium and a lower miscarriage rate than Clomiphene.
