微透析法测定青藤碱大鼠体外血浆蛋白结合率

Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.

微透析法进行盐酸青藤碱大鼠体外血浆蛋白结合率的测定及与超滤法的比较

目的:考察微透析技术进行蛋白结合率研究的可行性。方法:以微透析技术为采样手段,以HPLV-UV为检测工具,采用零净通量法测定了高、中、低浓度下盐酸青藤碱的大鼠离体血浆蛋白结合率,同时采用超滤法测其蛋白结合率,比较二者结果的异同。结果:微透析法测定的盐酸青藤碱大鼠蛋白结合率在所研究的浓度范围内保持了相对稳定,约为26%,与超滤法测定结果无显著区别。结论:盐酸青藤碱的蛋白结合率较低,微透析技术是可行的新型蛋白结合率研究方法。