It is to recognize glaucoma in view of Chinese medicine and synthesize the treatments and mechanisms of acupuncture on glaucoma in the paper. It is indicated in the results that acupuncture plays the importance in low...It is to recognize glaucoma in view of Chinese medicine and synthesize the treatments and mechanisms of acupuncture on glaucoma in the paper. It is indicated in the results that acupuncture plays the importance in lowering ocular tension, improving ocular circulation and protecting optic nerve. Additionally, acupuncture inhibits probably the cellular apoptosis of optic ganglion and provides definite actions on psychological factors of glaucoma. Acupuncture cannot replace operation and medicinal therapy in lowing ocular tension, but their coordinated and auxiliary effects are quite clear and definite.展开更多
In this study, the role of melanopsin-expressing retinal ganglion cells （mRGCs） in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five ...In this study, the role of melanopsin-expressing retinal ganglion cells （mRGCs） in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study. Immunohistochemical labeling, quantitative analysis of mRGC morphology, open field test （OFT）, and statistical analysis were used. In comparison with C57 BL/6 mice, the age-matched CFP-D2 mice had significantly elevated intraocular pressure （lOP）. We observed parallel morphological changes in the retina, including a reduction in the density of cyan fluorescent protein- （CFP） expressing cells （cells mm^-2 at 2 months of age, 1309±26; 14 months, 878±30, P〈0.001）, mRGCs （2 months, 48_＋3; 14 months, 19±4, P〈0.001）, Brn3b-expressing RGCs （2 months, 1283±80; 14 months, 950±31, P〈0.001）, Brn-3b expressing mRGCs （5 months, 50.17%±5.5%; 14 months, 12.61%±3.8%, P〈0.001）, and reduction in the dendritic field size of mRGCs （mm^2 at 2 months, 0.077±0.015; 14 months, 0.065±0.015, P〈0.05）. CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled, number of entries into the center, and time spent in the center of the testing apparatus. The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs, most likely Brn-3b-positive mRGCs in CFP-D2 mice.展开更多
文摘It is to recognize glaucoma in view of Chinese medicine and synthesize the treatments and mechanisms of acupuncture on glaucoma in the paper. It is indicated in the results that acupuncture plays the importance in lowering ocular tension, improving ocular circulation and protecting optic nerve. Additionally, acupuncture inhibits probably the cellular apoptosis of optic ganglion and provides definite actions on psychological factors of glaucoma. Acupuncture cannot replace operation and medicinal therapy in lowing ocular tension, but their coordinated and auxiliary effects are quite clear and definite.
基金supported by the National Basic Research Program of China (2009CB320900 to Pu MingLiang,2011CB510206 to Pu MingLiangand Gao Jie)National Natural Science Foundation of China(30831160516 to Pu MingLiang)+2 种基金NIH EY04067 (N.C. Brecha)VAMerit Review (N.C. Brecha).supported by a summer fellowship from the PKU-UCLA Joint Research Institute
文摘In this study, the role of melanopsin-expressing retinal ganglion cells （mRGCs） in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study. Immunohistochemical labeling, quantitative analysis of mRGC morphology, open field test （OFT）, and statistical analysis were used. In comparison with C57 BL/6 mice, the age-matched CFP-D2 mice had significantly elevated intraocular pressure （lOP）. We observed parallel morphological changes in the retina, including a reduction in the density of cyan fluorescent protein- （CFP） expressing cells （cells mm^-2 at 2 months of age, 1309±26; 14 months, 878±30, P〈0.001）, mRGCs （2 months, 48_＋3; 14 months, 19±4, P〈0.001）, Brn3b-expressing RGCs （2 months, 1283±80; 14 months, 950±31, P〈0.001）, Brn-3b expressing mRGCs （5 months, 50.17%±5.5%; 14 months, 12.61%±3.8%, P〈0.001）, and reduction in the dendritic field size of mRGCs （mm^2 at 2 months, 0.077±0.015; 14 months, 0.065±0.015, P〈0.05）. CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled, number of entries into the center, and time spent in the center of the testing apparatus. The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs, most likely Brn-3b-positive mRGCs in CFP-D2 mice.
