Objective： To observe the effect of the artesunate （ART） on cellular proliferation in vitro, to search for the possible anti-tumor mechanism of ART on endometrial carcinoma at the molecular level and to provide the...Objective： To observe the effect of the artesunate （ART） on cellular proliferation in vitro, to search for the possible anti-tumor mechanism of ART on endometrial carcinoma at the molecular level and to provide the experimental and theoretical foundations for the clinical applications of ART. Methods： The cell proliferation was observed by microscope; MTT was used to examine the effects of ART on proliferation of HEC-1B cells, and flow cytometric analysis was used to detect cell cycle and apoptosis. The human endometrial carcinoma HEC-1B cells were conventionally cultured; ART was administered with a concentration of 40 μg/ml before the total RNA were extracted, mRNA expression of Survivin, Caspase-3, N-Cadherin, E-Cadherin, Fibronectinl and Cox-2 were detected using RT-PCR. Results： ART reduced proliferation in human endometrial carcinoma cell line HEC-1B in a dose- and time-dependent effect. The cells of G0/G1 stage were significantly increased （P〈0.05）, but the cells of G2/M stages were significantly decreased （P〈0.05）, so it has shown that the cell cycle was probably blocked in G0/G1 stage. After intervention with ART at 20 and 80 μg/ml for 48 h, cellular apoptosis rate respectively was （36.42±0.77）% and （11.77±0.58）%, and the difference was statistically significant compared with the control （[6.64±0.191%, P〈0.01）. The expression of Cox-2 mRNA in the ART group was lower than those of control group, yet the expression of Caspase-3 and E-Cadherin mRNA in the ART group was higher than those of control group. Conclusion： ART can inhibit HEC-1B cell growth and proliferation in a dose- and time-dependent manner. Furthermore, ART can induce apoptosis in a dose-dependent manner. ART is able to downregulate Cox-2 mRNA expression and to upregulate E-Cadherin and Caspase-3 mRNA expression. So we can conclude that ART could induce the endometrial carcinoma HEC-1B cell apoptosis and inhibit tumor cell proliferation.展开更多
In the 1970 s, artemisinin(‘‘qinghaosu'' in Chinese), a sesquiterpene lactone with an unusual peroxide bridge, was isolated from Artemisia annua L. It showed promising antimalarial activity, particularly by ...In the 1970 s, artemisinin(‘‘qinghaosu'' in Chinese), a sesquiterpene lactone with an unusual peroxide bridge, was isolated from Artemisia annua L. It showed promising antimalarial activity, particularly by eliminating parasites resistant to chloroquine. For more than 30 years,artemisinin has contributed to worldwide health as a new type of antimalarial drug. Artemisinin and its analogs, such as dihydroartemisinin, artemether, artesunate, artemiside,artemisone, and arteether, possess not only potent antimalarial activity but also anti-viral, antifungal, anticancer,and anti-inflammatory properties. In this review, we discuss the current understanding of how artemisinin and its analogs affect the immune system and immune-related diseases.展开更多
文摘Objective： To observe the effect of the artesunate （ART） on cellular proliferation in vitro, to search for the possible anti-tumor mechanism of ART on endometrial carcinoma at the molecular level and to provide the experimental and theoretical foundations for the clinical applications of ART. Methods： The cell proliferation was observed by microscope; MTT was used to examine the effects of ART on proliferation of HEC-1B cells, and flow cytometric analysis was used to detect cell cycle and apoptosis. The human endometrial carcinoma HEC-1B cells were conventionally cultured; ART was administered with a concentration of 40 μg/ml before the total RNA were extracted, mRNA expression of Survivin, Caspase-3, N-Cadherin, E-Cadherin, Fibronectinl and Cox-2 were detected using RT-PCR. Results： ART reduced proliferation in human endometrial carcinoma cell line HEC-1B in a dose- and time-dependent effect. The cells of G0/G1 stage were significantly increased （P〈0.05）, but the cells of G2/M stages were significantly decreased （P〈0.05）, so it has shown that the cell cycle was probably blocked in G0/G1 stage. After intervention with ART at 20 and 80 μg/ml for 48 h, cellular apoptosis rate respectively was （36.42±0.77）% and （11.77±0.58）%, and the difference was statistically significant compared with the control （[6.64±0.191%, P〈0.01）. The expression of Cox-2 mRNA in the ART group was lower than those of control group, yet the expression of Caspase-3 and E-Cadherin mRNA in the ART group was higher than those of control group. Conclusion： ART can inhibit HEC-1B cell growth and proliferation in a dose- and time-dependent manner. Furthermore, ART can induce apoptosis in a dose-dependent manner. ART is able to downregulate Cox-2 mRNA expression and to upregulate E-Cadherin and Caspase-3 mRNA expression. So we can conclude that ART could induce the endometrial carcinoma HEC-1B cell apoptosis and inhibit tumor cell proliferation.
基金supported by the National Natural Science Foundation of China (91529305, 81427805, 81302507, 81302809, 31401611, 81573161, and 81502122)the Ministry of Science and Technology of China (2014AA020524)+2 种基金the CAS/ SAFEA International Partnership Program for Creative Research Teams of the Chinese Academy of Sciencesthe Science and Technology Commission of Shanghai Municipality (14391901800)the Key Laboratory of Food Safety Research of INS, SIBS, CAS
文摘In the 1970 s, artemisinin(‘‘qinghaosu'' in Chinese), a sesquiterpene lactone with an unusual peroxide bridge, was isolated from Artemisia annua L. It showed promising antimalarial activity, particularly by eliminating parasites resistant to chloroquine. For more than 30 years,artemisinin has contributed to worldwide health as a new type of antimalarial drug. Artemisinin and its analogs, such as dihydroartemisinin, artemether, artesunate, artemiside,artemisone, and arteether, possess not only potent antimalarial activity but also anti-viral, antifungal, anticancer,and anti-inflammatory properties. In this review, we discuss the current understanding of how artemisinin and its analogs affect the immune system and immune-related diseases.
