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转接方法和青霉素处理对葡萄试管苗芽孢杆菌抑制效应的研究
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作者 周晓康 李贻华 《落叶果树》 2004年第5期4-6,共3页
研究了用不同节位材料转接处理和不同浓度青霉素处理挽救在继代中被芽孢杆菌污染的葡萄试管苗的效果。结果表明 ,培养基灭完菌后未凝固时每升培养基中加入 5 0ml 3 2万单位 /L的青霉素钠溶液 ,能显著抑制芽孢杆菌发生 ,且试管苗生长正... 研究了用不同节位材料转接处理和不同浓度青霉素处理挽救在继代中被芽孢杆菌污染的葡萄试管苗的效果。结果表明 ,培养基灭完菌后未凝固时每升培养基中加入 5 0ml 3 2万单位 /L的青霉素钠溶液 ,能显著抑制芽孢杆菌发生 ,且试管苗生长正常。灭菌前加入青霉素的处理方法 ,虽对芽孢杆菌的抑制作用很强 ,但对试管苗的生长有明显抑制作用。经各种方法处理后获得的无芽孢材料 ,转接到改良B5培养基上均能正常生长且无芽孢再发生。 展开更多
关键词 葡萄试管苗 芽孢杆菌 转接方法 青霉素处理 抑制效应 组织培养
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青霉素处理检查和分离蓝藻细胞液泡
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作者 郭碧薇 易平 +1 位作者 刘希玲 郭厚良 《武汉植物学研究》 CSCD 2003年第3期273-275,共3页
Growing in the liquid medium containing penicillin, the cells of the Cyanobacteria, Anabaena 7120, Nostoc flagelliforme, and Synechocystis 6803 were broken and vacuoles were released. Percentage of broken cells declin... Growing in the liquid medium containing penicillin, the cells of the Cyanobacteria, Anabaena 7120, Nostoc flagelliforme, and Synechocystis 6803 were broken and vacuoles were released. Percentage of broken cells declined and percentage of broken cells increased with the growing days of the algae. The percentage of vacuoles to broken cells were respectively 0.7%, 0 8%, and 13.3% in the three types of algae Anabaena 7120, N.flagelliforme and Synechocystis 6803 which had grown for 3 days. 展开更多
关键词 蓝藻 青霉素处理 细胞液泡 分离
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Nonfoaming Bubble Separation for Recovery of Butyl Acetate from Discharged Wastewater During Penicillin Production 被引量:3
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作者 孙兴华 常志东 +2 位作者 胡欣 申淑锋 刘会洲 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2005年第3期329-333,共5页
Two nonfoaming bubble separation techniques, air stripping and solvent sublation, are presented and discussed in order to recover butyl acetate (BA) from discharged wastewater after solvent extraction of penicillin. R... Two nonfoaming bubble separation techniques, air stripping and solvent sublation, are presented and discussed in order to recover butyl acetate (BA) from discharged wastewater after solvent extraction of penicillin. Results show air stripping is not suitable for the recovery of BA from the wastewater. Axial concentration of BA had a noted maximum point along the column. In contrast, solvent sublation is very effective to recover BA from the wastewater. In solvent sublation experiments, axial concentration of BA along the column first increased and then decreased from the bottom to the top because of two primary mass transport processes. One is the transport by adsorption or attachment to ascending bubbles, and the other is by dispersion at water-solvent interface and by water film in organic solvent layer. In order to elucidate the high removal efficiency in solvent sublation, the microstructure of the wastewater was studied with optic microscope, which was showed to be an emulsion of BA in water at large concentration of BA. Solvent sublation can be successfully used in the removal of BA from its emulsion in the wastewater. The surface tension of simulated solution composed of lysozyme and BA was studied to understand mutual effect of biological materials and BA. Results show that lysozyme affects the adsorption of BA at air-water interface and they may form a complex between BA and lysozyme molecules. 展开更多
关键词 bubble separation air stripping solvent sublation butyl acetate WASTEWATER EMULSION
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