何静带我们与《时光的邂逅》

2009年炎热的夏季,一个动人的声音萦绕在耳际,在我们的心底激起了美丽的涟漪。那正是何静与爱女JuoJuo一起倾情演唱的《时光的邂逅》。那传递着母女情深的歌声是那么温暖。

避静带给我心灵回归

1995年4月6～8日,基督苦难主日前夕,北京西什库本堂神父组织了一次避静。感谢无限仁慈的天主,也恩赐了我避静的机会。三天时间比较紧张,除念经、听道理、默想外,就是自我省察,最后是避静神功。

带锁髓内钉静力固定改为动力固定治疗骨不连7例报告

传统加压钢板内固定由于常伴有应力遮挡和各种骨折的发生,临床应用近年来有减少趋势[1],而交锁髓内钉固定已经成为四肢长管骨骨折治疗的首选[2].作者2001年4月～2003年12月对68例股骨骨折的病人行带锁髓内钉内固定术,但术后半年发现有5例骨不连,另有2例外院手术治疗后骨不连.改动力固定后,效果满意.

静力型带锁髓内钉治疗不稳定胫骨骨折

目的:探讨静力型带锁髓内钉治疗不稳定胫骨骨折的效果。方法对我院自2006年3月~2011年3月采用静力型交锁髓内钉治疗不稳定型胫骨骨折42例进行回顾性分析。结果42例患者中38例获得随访。愈合时间16~42w,平均28w。随访时间16~28个月,平均21个月。膝关节功能满意。结论采用静力型锁定髓内钉治疗胫骨骨折患者愈合率很高,是治疗胫骨骨折较理想的内固定方法。

许美静高唱“快乐无罪”

一年没去台的许美静带着全新专辑《快乐无罪》,以一身前卫的“生化科技”造型在去年底登台,再次掀起歌迷的注目。早先由于身份问题无法入台的她,这回喜孜孜地重新亮相,

下肢长骨带锁髓内钉适时动力化的应用体会

为了对交锁髓内钉的动力化问题有更好的认识,本院收集2004年6月至2008年6月期间,采用静力型带锁髓内钉治疗下肢长管状骨骨干骨折的患者中随访资料较全者135例，改动力化固定47例，对其随诊资料进行了回顾总结。

带锁髓内钉治疗股骨干骨折失败一例

患者男，40岁。因重物砸伤左大腿致左股骨干中下段粉碎性骨折，急诊于硬膜外麻醉下行切开复位静力型带锁髓内钉、钢丝内固定术，术后X线片示骨折断端复位满意，无间隙。术后3d扶拐下地活动，7d活动膝关节防止僵硬。1个月后复查X线片发现骨折断端出现间隙，中间少量碎骨。3个月后复查断端间隙无明显生长迹象，

南海北部磁异常特征及对前新生代构造的指示

为了研究南海北部前新生代构造,利用新近的船载磁力测量数据,对磁异常进行变纬度化极,并反演计算视磁化强度和磁源重力异常,以及对三条OBS剖面进行重磁拟合.结果认为东沙隆起高磁异常带是浙闽沿海火山岩带向西的延续,其间被NW向古老的转换边界断裂F10错断;NE向的F2断裂是高磁异常带的南界,并限制了底侵活动的北界;F3断裂在拉伸减薄前是一个薄弱带,两侧深部热状态存在差异,极有可能是晚中生代古俯冲缝合带位置.磁静区在F2和F3断裂之间的磁性层磁性减弱,主要原因是底侵活动引起的热蚀变作用影响;而在F3断裂南侧磁性层磁性较强且较为破碎,斜磁化下磁异常正负相互压制是该区形成弱正磁异常的主要原因,该区磁性层的独特特征也可能反映了"古洋壳"的存在.

铺管设备电控系统的研究

主要阐述了S-LAY铺管设备的电控系统控制方式,实施细则以及现场调试解决方案,通过A&R绞车仿真移船功能,解决铺管作业中关键技术,实现驾控台对铺管设备远程控制的手段,以达到铺管设备作业的自动控制。

An anomaly in persistent right umbilical vein of portal vein diagnosed by ultrasonography

AIM: To detect the anomaly in the persistent right umbilical vein (PRUV) of portal vein (PV) with deviation of the ligamentum tere and left-sided gallbladder. METHODS: A total of 5783 candidates for routine analysis were evaluated for hepatic vascular abnormalities by ultrasonography. RESULTS: Ten candidates (0.17%) had a portal vein anomaly with a rightward-deviated ligamentum tere. The blood-flow velocity in the PRUV of the portal vein (17.7±3.0 cm/s) of the 10 cases was similar to that of the right anterior portal trunk (17.6±4.1 cm/s). However, the vessel diameter of the PRUV (φ12.4±4.4 mm) was larger than the right anterior portal trunk (φ6.1?.9 mm). Therefore, flow volume in the anomalous portion (0.97±0.30 L/min) was more than that in the right anterior portal trunk (0.18±0.05 L/min). CONCLUSION: The anomaly plays an important role in intra-hepatic PV flow.

Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells

AIM: To evaluate the killing effect of double suicide gene mediated by adenovirus and regulated under kinase domain insert containing receptor (KDR) promoter on human umbilical vein endothelial cells. METHODS: By PCR technology, human KDR promoter gene, Escherichia coli(E. coli) cytosine deaminase (CD) gene and the herpes simple virus-thymidine kinase (TK) gene were cloned. Plasmid pKDR-CDglyTK was constructed with them. Then, a recombinant adenoviral plasmid pAdKDRCDglyTK was constructed in a 'two-step transformation protocol'. The newly constructed plasmids were transfected to 293 packaging cells to grow adenoviruses, which were further propagated and purified. Human umbilical vein endothelial cells (HUVEC) were infected with a different multiplicity of infection (MOI) of resultant recombinant adenovirus, the infection rate was measured with the aid of (GFP) expression. Infected cells were cultured in culture media containing different concentrations of (GCV) and/or 5-(FC), and the killing effects were measured.RESULTS: Recombinant adenoviruses AdKDR-CDglyTK were successfully constructed, and they infected HUVEC cells efficiently. Our data indicated that the infection rate was relevant to MOI of recombinant adenoviruses. HUVEC cells infected with AdKDR-CDglyTK were highly sensitive to the prodrugs, their survival rate correlated to both the concentration of the prodrugs and the MOI of recombinant adenoviruses. Our data also indicated that the two prodrugs used in combination were much more effective on killing transgeneic cells than GCV or 5-FC used alone. CONCLUSION: Prodrug/KDR-CDglyTK system is effective on killing HUVEC cells, its killing effect correlates to the concentration of prodrugs and recombinant adenovirus' MOI. Combined use of the two prodrugs confers better killing effects on transgeneic cells.

Immortalization of human umbilical vein endothelial cells with telomerase reverse transcriptase and simian virus 40 large T antigen

Objective: To establish normally conditionally-immortalized human umbilical vein endothelial cells (HUVECs) by ectopic expression of the human telomerase catalytic enzyme (hTERT) and simian virus 40 large T (SV40 LT) antigen. Methods:Primary HUVECs were transfected with recombinant retrovirus containing hTERT or SV40 LT respectively. Subsequently drug resistant cell clones were screened and expanded for further studies. Endothelial cell biomarkers were confirmed by examination.Results: The morphological phenotype of the transfected cells was similar to the non-transfected cells. Von Willebrand factor,hTERT and SV40 LT could be detected in transfected HUVECs. Moreover, higher telomerase activity in transfected cells was maintained for over 50 population doublings compared with only low level of endogenous telomerase transiently at early population doublings in primary HUVECs. When exposed to TNF-α (tumor necrosis factor-α), the expression of E-selectin in transfected cells was significantly up-regulated, but no alteration of endothelial lipase was found. Conclusion: Ectopic coexpression of hTERT and SV40 LT can effectively immortalize HUVECs without tumorigenicity in vitro. Immortalized HUVECs may be an ideal target of further molecular function studies.

Effects of static magnetic field on human umbilical vessel endothelial cell

Objective: To investigate the effects of static magnetic field(SMF) on the viability, adhesion molecule expression of human umbilical vessel endothelial cell. Methods: Magnetic flux intensity was 0. 1 mT, 1 mT, 10 mT. Cell viability and proliferation were measured with 3H-TdR and MTT methods; and apoptosis of human umbilical vein endothelial cell (HUVEC) was studied by flow cytometry and transmission electric microscopy. ELISA was used to measure the expression of ICAM-1 and VCAM-1 on endothelium. Results: 0. 1 mT SMF had no effects on the growth of HUVEC. however,SMF of 1 mT, 10 mT attenuated growth of HUVEC. 10 mT static magnetic field could induce apoptosis and necrosis of HUVEC. 10 mT SMF enhanced the expression of ICAM-1 and VCAM-1 on endothelium. Conclusion: The effect of SMF depends on the intensity of SMF. 10 mT SMF has adverse effects on human umbilical vessel endothelial cell.

Electrostatic force on the rectangular shield wall of a strip transmission line

For a strip transmission line shielded by rectangular walls, the Green抯 function is helpful to construct the variation expression of the electrostatic energy. Thomson theorem is employed to determine the charge distribution on the strip. The electrostatic force on each side of the rectangular shield wall is achieved by using the principle of virtual work. The result is easy to be obtained by computerized calculation.

Mechanism Study of Endothelial Protection and Inhibits Platelet Activation of Low Molecular Weight Fucoidan from Laminaria japonica

Several studies have indicated that fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica could inhibit the activation of platelets directly by reducing the platelet aggregation. To explore the direct effect of LMW fucoidan on the platelet system furthermore and examine the possible mechanism, the endothelial protection and inhibits platelet activation effects of two LMW fucoidan were investigated. In the present study, Endothelial injury model of rats was made by injection of adrenaline(0.4 mg kg-1) and human umbilical vein endothelial cells were cultured. v WF level was be investigated in vivo and in vitro as an important index of endothelial injury. LMW fucoidan could significantly reduce v WF level in vascular endothelial injury rats and also significantly reduce v WF level in vitro. The number of EMPs was be detected as another important index of endothelial injury. The results showed that LMW fucoidan reduced EMPs stimulated by tumor necrosis factor. In this study, it was found that by inhibiting platelet adhesion, LMW fucoidan played a role in anti-thrombosis and the specific mechanism of action is to inhibit the flow of extracellular Ca2+. All in a word, LMW fucoidan could inhibit the activation of platelets indirectly by reducing the concentration of EMPs and v WF, at the same time; LMW fucoidan inhibited the activation of platelets directly by inhibiting the flow of extracellular Ca2+.

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminariajaponica

A rapid, sensitive and reproducible high performance liquid chromatography （HPLC） method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular- weight sulfated polysaccharide （GFS） in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows： half-time of distribution phase （t1/2α）=11.2±2.93 min, half-time of elimination phase （tl/2α）=98.20±25.78 min, maximum concentration （Cmax）=110.53 gg/mL and peak time （Tmax）=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration （200 mg/kg body weight）. Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with post-column derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.

Is transient elastography a useful tool for screening liver disease?

Transient elastography(TE)is a new non invasive tool for measuring liver stiffness,which is correlated to the histologic stage of liver fibrosis.Several studies in chronic liver disease(CLD)have determined a good accuracy of TE in predicting significant fibrosis and an optimal accuracy in predicting cirrhosis.Normal liver stiffness ranges between 3.3-7.8 KPa and using a cut off of 7.1 KPa,significant fibrosis and cirrhosis can be excluded with a very high negative predictive value(NPV).Positive predictive value(PPV)for the diagnosis of cirrhosis is lower using just a single scan but increases to 90% if high stiffness values are confirmed by a second independent scan.However the presence of fatty liver and metabolic syndrome slightly increases the readings and may reduce the accuracy of the test.It is uncertain if this increase is related to the presence of steatofibrosis or ifit is caused by steatosis itself.TE can be used in screening patients attending the liver clinics to identify those with signifi cant fi brosis or cirrhosis and may be particularly useful in discriminating HBV inactive carriers from chronic hepatitis B patients.TE,however,is not reliable in predicting the presence of esophageal varices in cirrhotics.Another potential indication for TE is the systematic screening of populations at high risk for CLD,such as intravenous drug users and alcoholics,but further studies are needed to determine its diagnostic accuracy in these settings.

Possible Thermal Brightness Temperature Anomalies Associated with the Yutian (China) M_S7.3 Earthquake on February 12,2014

In order to analyze the seismic brightness temperature anomalies associated with the Yutian earthquake which occurred at Yutian County, Xinjiang on February 12,2014, daily brightness temperature data was collected from the China Geostationary Meteorological Satellite FY-2E,for the period from May 1,2012 to April 30,2014 and the geographical extent of 30°- 45°N latitude and 70°- 95°E longitude. The continuous wavelet transform method was used to analyze the relative wavelet power spectrum( RWPS) of brightness temperature data for each pixel. And the RWPS time-spatial evolution within the analysis area was obtained. The results showed that the anomaly started to appear at the vicinity of epicentre since October 2013, and anomalous areas gradually enlarged and stretched towards to Altun fault zone and the eastern part of West Kunlun fault zone. Anomalies began to appear at fault zones at Middle Tianshan Mountains, Southern Tianshan Mountains and the western part of the West Kunlun Mountains area which is located at the western margin of Tarim basin,since November 2013. Then anomalous area further enlarged and gathered along fault zones,and eventually,anomalous belts were developed along fault zones around the Tarim basin. The anomaly area and amplitude reached their maximum in late December 2013 and early January 2014. With the impending earthquake,the anomaly area and amplitude dwindled. Anomalies at the vicinity of epicentre disappeared days before the occurrence of the main shock. However, the anomaly at Altun and Middle Tianshan areas still remained. After the main shock,the anomaly attenuated quickly and the whole anomaly disappeared in late February 2014.

Exact Solution to the Equation of Motion of a Charged Particle Driven by an Electrostatic Wave

An exact solution is derived for the equation of motion of a charged particle driven by an electrostatic wave.The explicit expression of particle velocity is obtained,and the trapping condition of the charged particle in the electrostatic wave is also derived exactly.The interaction between the charged particle and the electrostatic wave is discussed,which is a supplement to the existing textbook of plasma physics.The results are of interest to particle accelerators,microwave tubes,and basic plasma processes.