三阴性乳腺癌（triple negative breast carcinoma,TNBC）是指乳腺癌细胞的雌激素受体、孕激素受体和人表皮生长因子受体-2为阴性的一类乳腺癌[1]。TNBC因具有侵袭性强、缺乏有效的靶向治疗和内分泌治疗,是乳腺癌分子亚型中预后最差的...三阴性乳腺癌（triple negative breast carcinoma,TNBC）是指乳腺癌细胞的雌激素受体、孕激素受体和人表皮生长因子受体-2为阴性的一类乳腺癌[1]。TNBC因具有侵袭性强、缺乏有效的靶向治疗和内分泌治疗,是乳腺癌分子亚型中预后最差的一种。本文通过对三阴性乳腺癌与非三阴性乳腺癌的常规超声、超声弹性成像特点进行分析,有助于减少临床医生对三阴性乳腺癌的误诊。展开更多
Triple-negative breast cancer is the tumor that lacks expressions of estrogen receptor（ER）, progesterone receptor（PR） and human epidermal growth factor receptor-2（HER2）. A regular chemotherapy cannot eradicate t...Triple-negative breast cancer is the tumor that lacks expressions of estrogen receptor（ER）, progesterone receptor（PR） and human epidermal growth factor receptor-2（HER2）. A regular chemotherapy cannot eradicate triple-negative breast cancer. In the present study, we aimed to develop a combined use of daunorubicin and rofecoxib to treat triple-negative breast cancer, and reveal the underlying mechanisms. A gradient elution HPLC-UV method was developed for quantification, and the evaluations were performed on the triple-negative breast cancer MDA-MB-231 cells using a high content screening system. The results demonstrated that daunorubicin alone was insensitive to the triple negative breast cancer cells, while the combined use of daunorubicin and rofecoxib was able to effectively kill these triple-negative cancer cells, exhibiting a rofecoxib concentration-dependent manner. The mechanism revealed that the augmented anticancer efficacy was associated with direct killing effect, inducing apoptosis and inducing autophagy by the combination treatment. Besides, the apoptosis signaling pathways were correlated to a cascade of reactions by activating apoptotic enzyme caspase family and by suppressing anti-apoptotic gene expressed protein Bcl-2 family. In conclusion, this study provided a fundamental evidence for further developing the combined use of daunorubicin and rofecoxib formulation, hence offering a promising strategy for eradicating the triple negative breast cancer.展开更多
A metal-organic framework （MOF） has been obtained by using a linker extension strategy. Three di-anions of 4-（3,5-dimethyl- 1H-pyrazol-4-yl）-benzoic acid coordinate to three Cu（I） ions forming an extended trigon...A metal-organic framework （MOF） has been obtained by using a linker extension strategy. Three di-anions of 4-（3,5-dimethyl- 1H-pyrazol-4-yl）-benzoic acid coordinate to three Cu（I） ions forming an extended trigonal planar ligand, which links three di-copper paddlewheel units giving rise to a Pt304 net.展开更多
文摘三阴性乳腺癌（triple negative breast carcinoma,TNBC）是指乳腺癌细胞的雌激素受体、孕激素受体和人表皮生长因子受体-2为阴性的一类乳腺癌[1]。TNBC因具有侵袭性强、缺乏有效的靶向治疗和内分泌治疗,是乳腺癌分子亚型中预后最差的一种。本文通过对三阴性乳腺癌与非三阴性乳腺癌的常规超声、超声弹性成像特点进行分析,有助于减少临床医生对三阴性乳腺癌的误诊。
基金National Natural Science Foundation of China(Grant No.81373343)the Key Grant of Beijing Natural Science Foundation(Grant No.7131009)
文摘Triple-negative breast cancer is the tumor that lacks expressions of estrogen receptor（ER）, progesterone receptor（PR） and human epidermal growth factor receptor-2（HER2）. A regular chemotherapy cannot eradicate triple-negative breast cancer. In the present study, we aimed to develop a combined use of daunorubicin and rofecoxib to treat triple-negative breast cancer, and reveal the underlying mechanisms. A gradient elution HPLC-UV method was developed for quantification, and the evaluations were performed on the triple-negative breast cancer MDA-MB-231 cells using a high content screening system. The results demonstrated that daunorubicin alone was insensitive to the triple negative breast cancer cells, while the combined use of daunorubicin and rofecoxib was able to effectively kill these triple-negative cancer cells, exhibiting a rofecoxib concentration-dependent manner. The mechanism revealed that the augmented anticancer efficacy was associated with direct killing effect, inducing apoptosis and inducing autophagy by the combination treatment. Besides, the apoptosis signaling pathways were correlated to a cascade of reactions by activating apoptotic enzyme caspase family and by suppressing anti-apoptotic gene expressed protein Bcl-2 family. In conclusion, this study provided a fundamental evidence for further developing the combined use of daunorubicin and rofecoxib formulation, hence offering a promising strategy for eradicating the triple negative breast cancer.
基金supported by the U.S. Department of Energy (DOE Grants DE-FC36-07GO17033)the Welch Foundation (A-1725)+1 种基金supported by the National Science Foundation/Department of Energy under grant number NSF/CHE-0822838supported by the U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357
文摘A metal-organic framework （MOF） has been obtained by using a linker extension strategy. Three di-anions of 4-（3,5-dimethyl- 1H-pyrazol-4-yl）-benzoic acid coordinate to three Cu（I） ions forming an extended trigonal planar ligand, which links three di-copper paddlewheel units giving rise to a Pt304 net.
