Akt and Bcl-xL both promote resistance to apoptosis. A comparison of Akt- and Bcl-xL-dependent cell survival was undertaken. Expression of constitutively active Akt allows cells to survive for prolonged periods in the...Akt and Bcl-xL both promote resistance to apoptosis. A comparison of Akt- and Bcl-xL-dependent cell survival was undertaken. Expression of constitutively active Akt allows cells to survive for prolonged periods in the absence of growth factors. This survival correlates with the expression level of activated Akt and is comparable in magnitude to the protection provided by the anti-apoptotic gene Bcl-xL. Although both genes prevent cell death, Akt-protected cells can be distinguished from Bcl-xL-protected cells on the basis of increased glucose transporter expression, glycolytic activity, mitochondrial potential, and cell size. In addition, Akt-expressing cells require high levels of extracellular nutrients to support cell survival.展开更多
文摘Akt and Bcl-xL both promote resistance to apoptosis. A comparison of Akt- and Bcl-xL-dependent cell survival was undertaken. Expression of constitutively active Akt allows cells to survive for prolonged periods in the absence of growth factors. This survival correlates with the expression level of activated Akt and is comparable in magnitude to the protection provided by the anti-apoptotic gene Bcl-xL. Although both genes prevent cell death, Akt-protected cells can be distinguished from Bcl-xL-protected cells on the basis of increased glucose transporter expression, glycolytic activity, mitochondrial potential, and cell size. In addition, Akt-expressing cells require high levels of extracellular nutrients to support cell survival.
