A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was bette...A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in ail of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.展开更多
Observations of accumulated precipitation are extremely valuable for effectively improving rainfall analysis and forecast. It is, however, difficult to use such observations directly through sequential assimilation me...Observations of accumulated precipitation are extremely valuable for effectively improving rainfall analysis and forecast. It is, however, difficult to use such observations directly through sequential assimilation methods, such as three-dimensional variational data assimilation or an Ensemble Kalman Filter. In this study, the authors illustrate a new approach that makes effective use of precipitation data to improve rainfall forecast. The new method directly obtains an optimal solution in a reduced space by fitting observations with historical time series generated by the model; it also avoids the implementation of tangent linear model and its adjoint. A lot of historical samples are produced as the ensemble of precipitation observations with the fully nonlinear forecast model. The results show that the new approach is capable of extracting information from precipitation observations to improve the analysis and forecast. This method provides comparable performance with the standard fourdimensional variational data assimilation at a much lower computational cost.展开更多
The binding of (-)-epigallocatechin-3-O-gallate (EGCG) to bovine serum albumin (BSA) were investigated for the first time with spectral methods, including fluorescence and absorption spectrometry under simulativ...The binding of (-)-epigallocatechin-3-O-gallate (EGCG) to bovine serum albumin (BSA) were investigated for the first time with spectral methods, including fluorescence and absorption spectrometry under simulative physiological conditions. A strong fluorescence quenching reaction of EGCG to BSA was observed. It was proved that the fluorescence quenching of BSA by addition of EGCG was a result of the formation of EGCG-BSA complex. The binding constant K and the number of binding sites n were determined at physiological conditions and three different temperatures with fluorescence quenching method. The binding distance R and transfer efficiency E between BSA and EGCG were also obtained according to Forster theory of non-radiation energy transfer. The effects of Al^3+, Cu^2+, Mg^2+ and Fe^2+ on the binding constant between EGCG and BSA were studied at 298 K. The effect of EGCG on the conformation of BSA was also analyzed by synchronous fluorescence spectroscopy. PACS. 21. 10. Dr; 32. 50. +d; 32. 30. Jc; 82.80. Dx展开更多
Assume that each completely irrational noncommutative torus is realized as an inductive limit of circle algebras, and that for a completely irrational noncommutative torus Aω of rank m there are a completely irration...Assume that each completely irrational noncommutative torus is realized as an inductive limit of circle algebras, and that for a completely irrational noncommutative torus Aω of rank m there are a completely irrational noncommutative torus Aρ of rank m and a positive integer d such that tr(Aω) = tr(Aρ). It is proved that the set of all C*-algebras of sections of locally trivial C*-algebra bundles over S2 with fibres Aω. has a group structure, denoted by π1(Aut(Aω.)), which is isomorphic to Z if d > 1 and {0} if d > 1. Let Bcd be a cd-homogeneous C*-algebra over S2 x T2 of which no non-trivial matrix algebra can be factored out. The spherical noncommutative torns Sρcd is defined by twisting C*(T2 x Zm-2) in Bcd C* (Z(m-2)) by a totally skew multiplier ρ on T2 x Z(m-2). It is shown that Sρcd Mp∞ is isomorphic to C(S2) C* (T2 x Zm-2, ρ) Mcd(C) Mp∞ if and only if the set of prime factors of cd is a subset of the set of prime factors of p.展开更多
In this paper, we generate all nonisomorphic tournaments of order at mostnine, all nonisomorphic almost regular tournaments of order 10 and all nonisomorphic regulartournaments of order 11. For each of these tournamen...In this paper, we generate all nonisomorphic tournaments of order at mostnine, all nonisomorphic almost regular tournaments of order 10 and all nonisomorphic regulartournaments of order 11. For each of these tournaments, we have given its score-list, connectivity,diameter, the minimal number of feedbacks, automorphisms and spectra. Moreover, we have verified thewell-known Kelly's Conjecture for n = 2k + 1 ≤ 11. And we also determine the n-universaltournaments for n ≤ 6. However, several related results are given and some related open problemsare raised.展开更多
The kinetic fractionation of open-water evaporation against the stable water isotope H_2 ^(18)O is an important mechanism underlying many hydrologic studies that use ^(18)O as an isotopic tracer. A recent in-situ meas...The kinetic fractionation of open-water evaporation against the stable water isotope H_2 ^(18)O is an important mechanism underlying many hydrologic studies that use ^(18)O as an isotopic tracer. A recent in-situ measurement of the isotopic water vapor flux over a lake indicates that the kinetic effect is much weaker(kinetic factor 6.2‰) than assumed previously(kinetic factor14.2‰) by lake isotopic budget studies. This study investigates the implications of the weak kinetic effect for studies of deuterium excess-humidity relationships, regional moisture recycling, and global evapotranspiration partitioning. The results indicate that the low kinetic factor is consistent with the deuterium excess-humidity relationships observed over open oceans.The moisture recycling rate in the Great Lakes region derived from the isotopic tracer method with the low kinetic factor is a much better agreement with those from atmospheric modeling studies than if the default kinetic factor of 14.2‰ is used. The ratio of transpiration to evapotranspiration at global scale decreases from 84±9%(with the default kinetic factor) to 76±19%(with the low kinetic factor), the latter of which is in slightly better agreement with other non-isotopic partitioning results.展开更多
Metric n-Lie algebras have wide applications in mathematics and mathematical physics. In this paper, the authors introduce two methods to construct metric (n+1)-Lie algebras from metric n-Lie algebras for n≥2. For a ...Metric n-Lie algebras have wide applications in mathematics and mathematical physics. In this paper, the authors introduce two methods to construct metric (n+1)-Lie algebras from metric n-Lie algebras for n≥2. For a given m-dimensional metric n-Lie algebra(g, [, ···, ], B_g), via one and two dimensional extensions £=g+IFc and g0= g+IFx^(-1)+IFx^0 of the vector space g and a certain linear function f on g, we construct(m+1)-and (m+2)-dimensional (n+1)-Lie algebras(£, [, ···, ]cf) and(g0, [, ···, ]1), respectively.Furthermore, if the center Z(g) is non-isotropic, then we obtain metric(n + 1)-Lie algebras(L, [, ···, ]cf, B) and(g0, [, ···, ]1, B) which satisfy B|g×g = Bg. Following this approach the extensions of all(n + 2)-dimensional metric n-Lie algebras are discussed.展开更多
In this paper, we find that under a diffeomorphic of nonlinear geodesic equations are concerned with light-like extremal surfaces in curved spaeetimes. It is interesting to transformation of variables, the light-like ...In this paper, we find that under a diffeomorphic of nonlinear geodesic equations are concerned with light-like extremal surfaces in curved spaeetimes. It is interesting to transformation of variables, the light-like extremal surfaces can be described by a system Particularly, we investigate the light-like extremal surfaces in Schwarzschild spacetime in detail and some new special solutions are derived systematically with aim to compare with the known results and to illustrate the method.展开更多
文摘A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in ail of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.
基金the Ministry of Finance of China and China Meteorological Administration for the Special Project of Meteorological Sector (Grant No. GYHY(QX)2007-615)the National Basic Research Program of China (Grant No. 2005CB321703)
文摘Observations of accumulated precipitation are extremely valuable for effectively improving rainfall analysis and forecast. It is, however, difficult to use such observations directly through sequential assimilation methods, such as three-dimensional variational data assimilation or an Ensemble Kalman Filter. In this study, the authors illustrate a new approach that makes effective use of precipitation data to improve rainfall forecast. The new method directly obtains an optimal solution in a reduced space by fitting observations with historical time series generated by the model; it also avoids the implementation of tangent linear model and its adjoint. A lot of historical samples are produced as the ensemble of precipitation observations with the fully nonlinear forecast model. The results show that the new approach is capable of extracting information from precipitation observations to improve the analysis and forecast. This method provides comparable performance with the standard fourdimensional variational data assimilation at a much lower computational cost.
文摘The binding of (-)-epigallocatechin-3-O-gallate (EGCG) to bovine serum albumin (BSA) were investigated for the first time with spectral methods, including fluorescence and absorption spectrometry under simulative physiological conditions. A strong fluorescence quenching reaction of EGCG to BSA was observed. It was proved that the fluorescence quenching of BSA by addition of EGCG was a result of the formation of EGCG-BSA complex. The binding constant K and the number of binding sites n were determined at physiological conditions and three different temperatures with fluorescence quenching method. The binding distance R and transfer efficiency E between BSA and EGCG were also obtained according to Forster theory of non-radiation energy transfer. The effects of Al^3+, Cu^2+, Mg^2+ and Fe^2+ on the binding constant between EGCG and BSA were studied at 298 K. The effect of EGCG on the conformation of BSA was also analyzed by synchronous fluorescence spectroscopy. PACS. 21. 10. Dr; 32. 50. +d; 32. 30. Jc; 82.80. Dx
基金Project supported by the grant No. 1999-2-102-001-3 from the Interdisciplinary Research Program Year of the KOSEF
文摘Assume that each completely irrational noncommutative torus is realized as an inductive limit of circle algebras, and that for a completely irrational noncommutative torus Aω of rank m there are a completely irrational noncommutative torus Aρ of rank m and a positive integer d such that tr(Aω) = tr(Aρ). It is proved that the set of all C*-algebras of sections of locally trivial C*-algebra bundles over S2 with fibres Aω. has a group structure, denoted by π1(Aut(Aω.)), which is isomorphic to Z if d > 1 and {0} if d > 1. Let Bcd be a cd-homogeneous C*-algebra over S2 x T2 of which no non-trivial matrix algebra can be factored out. The spherical noncommutative torns Sρcd is defined by twisting C*(T2 x Zm-2) in Bcd C* (Z(m-2)) by a totally skew multiplier ρ on T2 x Z(m-2). It is shown that Sρcd Mp∞ is isomorphic to C(S2) C* (T2 x Zm-2, ρ) Mcd(C) Mp∞ if and only if the set of prime factors of cd is a subset of the set of prime factors of p.
文摘In this paper, we generate all nonisomorphic tournaments of order at mostnine, all nonisomorphic almost regular tournaments of order 10 and all nonisomorphic regulartournaments of order 11. For each of these tournaments, we have given its score-list, connectivity,diameter, the minimal number of feedbacks, automorphisms and spectra. Moreover, we have verified thewell-known Kelly's Conjecture for n = 2k + 1 ≤ 11. And we also determine the n-universaltournaments for n ≤ 6. However, several related results are given and some related open problemsare raised.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41475141, 41830860, 41575147 & 41505005)the National Key Research and Development Program of China (Grant No. 2016YFC0500102)+5 种基金the U. S. National Science Foundation (Grant No. 1520684)the Science and Technology Department of Ningxia (Grant No. 2015KJHM34)the China Special Fund for Meteorological Research in the Public Interest (Major projects, Grant No. GYHY201506001-6)the NUIST Scientific Foundation (Grant No. KLME1415)the Priority Academic Program Development of Jiangsu Higher Education Institutions (Grant No. PAPD)the Ministry of Education of the People’s Republic of China (Grant No. PCSIRT)
文摘The kinetic fractionation of open-water evaporation against the stable water isotope H_2 ^(18)O is an important mechanism underlying many hydrologic studies that use ^(18)O as an isotopic tracer. A recent in-situ measurement of the isotopic water vapor flux over a lake indicates that the kinetic effect is much weaker(kinetic factor 6.2‰) than assumed previously(kinetic factor14.2‰) by lake isotopic budget studies. This study investigates the implications of the weak kinetic effect for studies of deuterium excess-humidity relationships, regional moisture recycling, and global evapotranspiration partitioning. The results indicate that the low kinetic factor is consistent with the deuterium excess-humidity relationships observed over open oceans.The moisture recycling rate in the Great Lakes region derived from the isotopic tracer method with the low kinetic factor is a much better agreement with those from atmospheric modeling studies than if the default kinetic factor of 14.2‰ is used. The ratio of transpiration to evapotranspiration at global scale decreases from 84±9%(with the default kinetic factor) to 76±19%(with the low kinetic factor), the latter of which is in slightly better agreement with other non-isotopic partitioning results.
基金supported by the National Natural Science Foundation of China(No.11371245)the Natural Science Foundation of Hebei Province(No.A2014201006)
文摘Metric n-Lie algebras have wide applications in mathematics and mathematical physics. In this paper, the authors introduce two methods to construct metric (n+1)-Lie algebras from metric n-Lie algebras for n≥2. For a given m-dimensional metric n-Lie algebra(g, [, ···, ], B_g), via one and two dimensional extensions £=g+IFc and g0= g+IFx^(-1)+IFx^0 of the vector space g and a certain linear function f on g, we construct(m+1)-and (m+2)-dimensional (n+1)-Lie algebras(£, [, ···, ]cf) and(g0, [, ···, ]1), respectively.Furthermore, if the center Z(g) is non-isotropic, then we obtain metric(n + 1)-Lie algebras(L, [, ···, ]cf, B) and(g0, [, ···, ]1, B) which satisfy B|g×g = Bg. Following this approach the extensions of all(n + 2)-dimensional metric n-Lie algebras are discussed.
基金Supported by National Natural Science Foundation of China under Grant Nos.11026151,11101001the Anhui Provincial University’s Natural Science Foundation under Grant No.KJ2010A130
文摘In this paper, we find that under a diffeomorphic of nonlinear geodesic equations are concerned with light-like extremal surfaces in curved spaeetimes. It is interesting to transformation of variables, the light-like extremal surfaces can be described by a system Particularly, we investigate the light-like extremal surfaces in Schwarzschild spacetime in detail and some new special solutions are derived systematically with aim to compare with the known results and to illustrate the method.