Objective: To detect the expression of CEA mRNA in patients with non-small cell lung cancer (NSCLC) and to investigate it's significance. Methods: The blood samples were taken from peripheral veins of 70 patients ...Objective: To detect the expression of CEA mRNA in patients with non-small cell lung cancer (NSCLC) and to investigate it's significance. Methods: The blood samples were taken from peripheral veins of 70 patients with NSCLC and 18 patients with benign diseases at 3 intervals during the surgery. The transcription of carcinoembryonic antigen messenger ribonucleic acid (CEA mRNA) was assayed by means of nested reverse transcriptase polymerase chain reaction (RT-PCR) and micro-fluid chip. Results: The CEA mRNA positive rates at each of the 3 time spots were as follows: 50% at beginning of the surgery (group 1), 62.8% in the samples collected when ligating the pulmonary vein (group 2) and 57.1% in samples collected 1 h after ligation (group 3). A significant difference was found between groups 1 and 2 (χ2 = 7.114, P < 0.05). Con-clusion: Cancer cell dissemination during surgery is demonstrated indirectly in our study, when to ligate the pulmonary vein (earlier or later) may affect the quantity of tumor cells spread into the circulation.展开更多
OBJECTIVE To explore the effects of cisplatin chemotherapy via the vein and the abdominal cavity on the functions of endothelial cells of mice with non-small cell lung cancer. METHODS 75 mice were divided equally into...OBJECTIVE To explore the effects of cisplatin chemotherapy via the vein and the abdominal cavity on the functions of endothelial cells of mice with non-small cell lung cancer. METHODS 75 mice were divided equally into a untreated group, a group treated with chemotherapy （cisplatin： 2 mg/kg, 0.5 mL） via an intravenous method and a group treated with chemotherapy （cisplatin： 2 mg/kg, 0.5 mL） via an intraperitoneal method; changes in the morpholoqy and ultrastructure of vein endothelial cells were observed.展开更多
基金the Natural Sciences Foundation of Anhui Province (No. 2002kj220)
文摘Objective: To detect the expression of CEA mRNA in patients with non-small cell lung cancer (NSCLC) and to investigate it's significance. Methods: The blood samples were taken from peripheral veins of 70 patients with NSCLC and 18 patients with benign diseases at 3 intervals during the surgery. The transcription of carcinoembryonic antigen messenger ribonucleic acid (CEA mRNA) was assayed by means of nested reverse transcriptase polymerase chain reaction (RT-PCR) and micro-fluid chip. Results: The CEA mRNA positive rates at each of the 3 time spots were as follows: 50% at beginning of the surgery (group 1), 62.8% in the samples collected when ligating the pulmonary vein (group 2) and 57.1% in samples collected 1 h after ligation (group 3). A significant difference was found between groups 1 and 2 (χ2 = 7.114, P < 0.05). Con-clusion: Cancer cell dissemination during surgery is demonstrated indirectly in our study, when to ligate the pulmonary vein (earlier or later) may affect the quantity of tumor cells spread into the circulation.
文摘OBJECTIVE To explore the effects of cisplatin chemotherapy via the vein and the abdominal cavity on the functions of endothelial cells of mice with non-small cell lung cancer. METHODS 75 mice were divided equally into a untreated group, a group treated with chemotherapy （cisplatin： 2 mg/kg, 0.5 mL） via an intravenous method and a group treated with chemotherapy （cisplatin： 2 mg/kg, 0.5 mL） via an intraperitoneal method; changes in the morpholoqy and ultrastructure of vein endothelial cells were observed.
