[Objective] The aim was to investigate the optimal conditions of SRAP molecular marker used in the analysis on Fagopyrum tataricum（L.）Gaertn.[Method]SRAP-PCR amplification system on Fagopyrum tataricum was optimized...[Objective] The aim was to investigate the optimal conditions of SRAP molecular marker used in the analysis on Fagopyrum tataricum（L.）Gaertn.[Method]SRAP-PCR amplification system on Fagopyrum tataricum was optimized by interactive orthogonal design L27（313）in 5 elements（Mg2＋,dNTP,Taq DNA polymerase,template DNA and primer）at 3 levels.And the non-denaturing and denaturing PAGE detection methods were compared.The comparative test of DYCZ-24F and DYCZ-20C electrophoresis operating systems was carried out.[Result]The effects of four single-factor（Mg2＋,dNTP,Taq DNA polymerase and primer）and two interactions（Mg2＋×dNTP,Mg2＋×Taq DNA polymerase）on tartary buckwheat SRAP-PCR were significant.An optimal reaction system was established containing 1.5 mmol/L Mg2＋,0.2 mmol/L dNTP,1.5 u Taq DNA polymerase,40 ng DNA,0.25 μmol/L primer and 2 μl 10×buffer.Seven samples of tartary buckwheat were amplified using this system,and electrophoresis results showed clear bands,high level of polymorphism and good reproducibility.The PCR products were tested by denaturing and non-denaturing PAGE,and the results showed that the non-denaturing PAGE,DYCZ-24F operating system was more suitable for SRAP analysis.[Conclusion]This study established a foundation for the construction of SRAP genetic map of tartary buckwheat.展开更多
Latin hypercube design and uniform design are two kinds of most popular space-filling designs for computer experiments. The fact that the run size equals the number of factor levels in a Latin hypercube design makes i...Latin hypercube design and uniform design are two kinds of most popular space-filling designs for computer experiments. The fact that the run size equals the number of factor levels in a Latin hypercube design makes it difficult to be orthogonal. While for a uniform design, it usually has good space-filling properties, but does not necessarily have small or zero correlations between factors. In this paper, we construct a class of column-orthogonal and nearly column-orthogonal designs for computer experiments by rotating groups of factors of orthogonal arrays, which supplement the designs for computer experiments in terms of various run sizes and numbers of factor levels and are flexible in accommodating various combinations of factors with different numbers of levels. The resulting column-orthogonal designs not only have uniformly spaced levels for each factor but also have uncorrelated estimates of the linear effects in first order models. Further, they are 3-orthogonal if the corresponding orthogonal arrays have strength equal to or greater than three. Along with a large factor-to-run ratio, these newly constructed designs are economical and suitable for screening factors for physical experiments.展开更多
基金Supported by National Natural Science Foundation of China(30771310)~~
文摘[Objective] The aim was to investigate the optimal conditions of SRAP molecular marker used in the analysis on Fagopyrum tataricum（L.）Gaertn.[Method]SRAP-PCR amplification system on Fagopyrum tataricum was optimized by interactive orthogonal design L27（313）in 5 elements（Mg2＋,dNTP,Taq DNA polymerase,template DNA and primer）at 3 levels.And the non-denaturing and denaturing PAGE detection methods were compared.The comparative test of DYCZ-24F and DYCZ-20C electrophoresis operating systems was carried out.[Result]The effects of four single-factor（Mg2＋,dNTP,Taq DNA polymerase and primer）and two interactions（Mg2＋×dNTP,Mg2＋×Taq DNA polymerase）on tartary buckwheat SRAP-PCR were significant.An optimal reaction system was established containing 1.5 mmol/L Mg2＋,0.2 mmol/L dNTP,1.5 u Taq DNA polymerase,40 ng DNA,0.25 μmol/L primer and 2 μl 10×buffer.Seven samples of tartary buckwheat were amplified using this system,and electrophoresis results showed clear bands,high level of polymorphism and good reproducibility.The PCR products were tested by denaturing and non-denaturing PAGE,and the results showed that the non-denaturing PAGE,DYCZ-24F operating system was more suitable for SRAP analysis.[Conclusion]This study established a foundation for the construction of SRAP genetic map of tartary buckwheat.
基金supported by the Program for New Century Excellent Talents in Universityof China (Grant No. NCET-07-0454)National Natural Science Foundation of China (Grant No. 10971107)the Fundamental Research Funds for the Central Universities (Grant No. 10QNJJ003)
文摘Latin hypercube design and uniform design are two kinds of most popular space-filling designs for computer experiments. The fact that the run size equals the number of factor levels in a Latin hypercube design makes it difficult to be orthogonal. While for a uniform design, it usually has good space-filling properties, but does not necessarily have small or zero correlations between factors. In this paper, we construct a class of column-orthogonal and nearly column-orthogonal designs for computer experiments by rotating groups of factors of orthogonal arrays, which supplement the designs for computer experiments in terms of various run sizes and numbers of factor levels and are flexible in accommodating various combinations of factors with different numbers of levels. The resulting column-orthogonal designs not only have uniformly spaced levels for each factor but also have uncorrelated estimates of the linear effects in first order models. Further, they are 3-orthogonal if the corresponding orthogonal arrays have strength equal to or greater than three. Along with a large factor-to-run ratio, these newly constructed designs are economical and suitable for screening factors for physical experiments.
