e抗原阴性慢性乙型肝炎病毒(hepatitis B virus,HBV)感染是世界范围内慢性乙型肝炎(chronic hepatitis B,CHB)的主要形式,其管理是世界公共卫生的重大负担。近年,对慢性HBV感染的进一步探索,使得CHB的管理更加精确。欧洲肝病学会提出将...e抗原阴性慢性乙型肝炎病毒(hepatitis B virus,HBV)感染是世界范围内慢性乙型肝炎(chronic hepatitis B,CHB)的主要形式,其管理是世界公共卫生的重大负担。近年,对慢性HBV感染的进一步探索,使得CHB的管理更加精确。欧洲肝病学会提出将CHB自然史分为以下5个阶段:HBeAg阳性HBV感染、HBeAg阳性慢性肝炎、HBeAg阴性HBV感染、HBeAg阴性慢性肝炎、HBeAg阴性HBV感染(隐匿性HBV感染),有相当比例的患者不属于以上任何一个分期,即不确定期。本文总结了关于e抗原阴性HBV感染的文献和指南,旨在帮助临床医师使用无创性工具(如乙肝表面抗原定量和弹性成像)评估HBeAg阴性非活动期与不确定期患者,并及时开始抗病毒治疗。展开更多
患者女,20岁,自述2年前无明显诱因反复头枕部及颈部疼痛,严重时伴有左上肢乏力,自觉颈部发热。近1个月开始持续疼痛。体格检查:颈动脉听诊可闻及血管杂音,上臂肱动脉处血压为65~82/44~64 mm Hg(1 mm Hg=0.133 kPa),下肢腘动脉处血压为13...患者女,20岁,自述2年前无明显诱因反复头枕部及颈部疼痛,严重时伴有左上肢乏力,自觉颈部发热。近1个月开始持续疼痛。体格检查:颈动脉听诊可闻及血管杂音,上臂肱动脉处血压为65~82/44~64 mm Hg(1 mm Hg=0.133 kPa),下肢腘动脉处血压为130/75 mm Hg。实验室检查:血沉28 mm/h,白细胞13.0×109/L,血小板223×109/L。超声检查:左侧颈总、颈内、颈动脉球部、颈外动脉内及右侧颈总动脉内-中膜弥漫性增厚,较厚处约3.2 mm(图1A),左侧颈总动脉接近闭塞.展开更多
Background/Aims:Hepatitis B virus (HBV) infection in extrahepatic tissues is controversial. To clarify whether episomal HBV can infect nonhepatic tissues, we investigated the molecular forms of HBV in the lymphatic ce...Background/Aims:Hepatitis B virus (HBV) infection in extrahepatic tissues is controversial. To clarify whether episomal HBV can infect nonhepatic tissues, we investigated the molecular forms of HBV in the lymphatic cells of inactive HBV carriers who lacked viremia, thus avoiding contamination with HBV genomes originating from the viral particles present in the serum. Methods:We assessed HBV genome, replicative forms, and viral integrants in the liver, serum, peripheral blood mononuclear cells (PBMC), and lymph nodes of 21 inactive HBV carriers who tested positive for antibodies against the HBV core antigen (anti-HBc). Results:Of the 21 anti-HBc positive individuals, HBV-DNA was detected in liver samples of 15 (71.4%), in the lymph nodes of 11 (52.4%), and in PBMC of three (14.3%). However, none of the detected HBV genomes from lymphatic tissues included the replicative forms of HBV. In one case, integrated HBV was present in the lymphatic tissues and the host-viral junction was present in the intronic sequences of chromosome 17. Conclusions:These data suggest that human lymphatic tissues cannot support viral replication in anti-HBc positive inactive HBV carriers, while retaining the viral genome as an integrated form.展开更多
文摘e抗原阴性慢性乙型肝炎病毒(hepatitis B virus,HBV)感染是世界范围内慢性乙型肝炎(chronic hepatitis B,CHB)的主要形式,其管理是世界公共卫生的重大负担。近年,对慢性HBV感染的进一步探索,使得CHB的管理更加精确。欧洲肝病学会提出将CHB自然史分为以下5个阶段:HBeAg阳性HBV感染、HBeAg阳性慢性肝炎、HBeAg阴性HBV感染、HBeAg阴性慢性肝炎、HBeAg阴性HBV感染(隐匿性HBV感染),有相当比例的患者不属于以上任何一个分期,即不确定期。本文总结了关于e抗原阴性HBV感染的文献和指南,旨在帮助临床医师使用无创性工具(如乙肝表面抗原定量和弹性成像)评估HBeAg阴性非活动期与不确定期患者,并及时开始抗病毒治疗。
文摘患者女,20岁,自述2年前无明显诱因反复头枕部及颈部疼痛,严重时伴有左上肢乏力,自觉颈部发热。近1个月开始持续疼痛。体格检查:颈动脉听诊可闻及血管杂音,上臂肱动脉处血压为65~82/44~64 mm Hg(1 mm Hg=0.133 kPa),下肢腘动脉处血压为130/75 mm Hg。实验室检查:血沉28 mm/h,白细胞13.0×109/L,血小板223×109/L。超声检查:左侧颈总、颈内、颈动脉球部、颈外动脉内及右侧颈总动脉内-中膜弥漫性增厚,较厚处约3.2 mm(图1A),左侧颈总动脉接近闭塞.
文摘Background/Aims:Hepatitis B virus (HBV) infection in extrahepatic tissues is controversial. To clarify whether episomal HBV can infect nonhepatic tissues, we investigated the molecular forms of HBV in the lymphatic cells of inactive HBV carriers who lacked viremia, thus avoiding contamination with HBV genomes originating from the viral particles present in the serum. Methods:We assessed HBV genome, replicative forms, and viral integrants in the liver, serum, peripheral blood mononuclear cells (PBMC), and lymph nodes of 21 inactive HBV carriers who tested positive for antibodies against the HBV core antigen (anti-HBc). Results:Of the 21 anti-HBc positive individuals, HBV-DNA was detected in liver samples of 15 (71.4%), in the lymph nodes of 11 (52.4%), and in PBMC of three (14.3%). However, none of the detected HBV genomes from lymphatic tissues included the replicative forms of HBV. In one case, integrated HBV was present in the lymphatic tissues and the host-viral junction was present in the intronic sequences of chromosome 17. Conclusions:These data suggest that human lymphatic tissues cannot support viral replication in anti-HBc positive inactive HBV carriers, while retaining the viral genome as an integrated form.
