Colony morphology is important for Microcystis to sustain a competitive advantage in eutrophic lakes. The mechanism of colony formation in Microcystis is currently unclear. Extracellular polysaccharide (EPS) has bee...Colony morphology is important for Microcystis to sustain a competitive advantage in eutrophic lakes. The mechanism of colony formation in Microcystis is currently unclear. Extracellular polysaccharide (EPS) has been reported to play an important role in cell aggregate formation of some phytoplankton. Microcystis aeruginosa was cultivated under varied abiotic conditions, including different nutrient, light, and temperature conditions, to investigate their effects on EPS production and morphological change. The results show that nutrient concentration and light intensity have great effects on EPS production in M. aeruginosa. There was a considerable increase in EPS production after M. aeruginosa was cultivated in adjusted culture conditions similar to those present in the field (28.9 mg C/L, 1.98 mg N/L, 0.65 mg P/L, light intensity: 100 μtmol/(m2.s)). These results indicate that abiotic factors might be one of the triggers for colony formation in Microcystis.展开更多
In this study, we aimed to investigate the influencing factors of postoperative 5-year survival rate in non small-cell lung cancer(NSCLC) patients with stage I–III. We enrolled 220 patients who underwent surgical o...In this study, we aimed to investigate the influencing factors of postoperative 5-year survival rate in non small-cell lung cancer(NSCLC) patients with stage I–III. We enrolled 220 patients who underwent surgical operation and survived longer than 3 months, with histologically confirmed adenocarcinoma or squamous cell carcinoma from Jan. 2009 to Dec. 2009. Multiple variables, like age, gender, smoking, alcohol, obesity, family history of cancer, diabetes, hypertension, antidiabetic drugs, tumor staging, pathology, treatments, surgical side as well as lipid levels, were selected. We found that tumor staging was associated with 5-year survival rate of stage I–III NSCLC patients by univariate analysis. Furthermore, diabetes mellitus, hypertension and surgical side were associated with the overall survival of NSCLC patients with stage I, stageⅡand stage III, respectively. In conclusion, the status of chronic disease had potential effect on the postoperative 5-year survival rate of lung cancer patients.展开更多
The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants. Different biotic and abiotie factors can affect the survival and activity of the applie...The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants. Different biotic and abiotie factors can affect the survival and activity of the applied bacteria and consequently plant growth and phy- toremediation efficiency. The effect of inoculum density on the abundance and expression of alkune-degrading genes in the rhizosphere of plant vegetated in hydrocarbon-contaminated soil has been rarely observed. In this study, an alkane-degrading bacterium (Pantoea sp. strain BTRH79), at different inoculum densities (10^5 to 10^8 cells cm^-3 soil), was inoculated to ryegrass (Lolium perenne) vegetated in diesel-contaminated soil to find the optimum inoculum density needed for its efficient colonization and hydrocarbon degradation activity. Bacterial inoculation improved plant growth and hydrocarbon degradation. Maximum plant growth and hydrocarbon degra- dation were observed with the inoculum having the highest cell density (10^8 cells cm^-3 soil). Moreover, the inoculum with higher cell density exhibited more abundance and expression of alkane hydroxylase gene, CYP153. This study suggests that the inoculum density is one of the main factors that can affect bacterial colonization and activity during phytoremediation.展开更多
基金Supported by the National Natural Science Foundation of China (No.31200296)the CAS International Partnership Creative Group (No.KZZD-EW-TZ-08-01)
文摘Colony morphology is important for Microcystis to sustain a competitive advantage in eutrophic lakes. The mechanism of colony formation in Microcystis is currently unclear. Extracellular polysaccharide (EPS) has been reported to play an important role in cell aggregate formation of some phytoplankton. Microcystis aeruginosa was cultivated under varied abiotic conditions, including different nutrient, light, and temperature conditions, to investigate their effects on EPS production and morphological change. The results show that nutrient concentration and light intensity have great effects on EPS production in M. aeruginosa. There was a considerable increase in EPS production after M. aeruginosa was cultivated in adjusted culture conditions similar to those present in the field (28.9 mg C/L, 1.98 mg N/L, 0.65 mg P/L, light intensity: 100 μtmol/(m2.s)). These results indicate that abiotic factors might be one of the triggers for colony formation in Microcystis.
基金General Research Program of Medical Health in Zhejiang Province(Grant No.2014KYB039)Zhejiang Provincial Program for the Zhejiang Medical and Health Science and Technology Program(Grant No.2015KYB071)+2 种基金Scientific Research Fund Project of Integrated Chinese and Western Medicine Institute in Zhejiang Province(Grant No.2014LYK021)Science and Technology in Zhejiang Province Chinese Medicine Program(Grant No.2016ZA038)1022 Talent Training Program of Zhejiang Cancer Hospital
文摘In this study, we aimed to investigate the influencing factors of postoperative 5-year survival rate in non small-cell lung cancer(NSCLC) patients with stage I–III. We enrolled 220 patients who underwent surgical operation and survived longer than 3 months, with histologically confirmed adenocarcinoma or squamous cell carcinoma from Jan. 2009 to Dec. 2009. Multiple variables, like age, gender, smoking, alcohol, obesity, family history of cancer, diabetes, hypertension, antidiabetic drugs, tumor staging, pathology, treatments, surgical side as well as lipid levels, were selected. We found that tumor staging was associated with 5-year survival rate of stage I–III NSCLC patients by univariate analysis. Furthermore, diabetes mellitus, hypertension and surgical side were associated with the overall survival of NSCLC patients with stage I, stageⅡand stage III, respectively. In conclusion, the status of chronic disease had potential effect on the postoperative 5-year survival rate of lung cancer patients.
基金supported by the Higher Education Commission (HEC), Pakistan (No. 20-2011-1997)
文摘The combined use of plants and bacteria is a promising approach for the remediation of soil contaminated with organic pollutants. Different biotic and abiotie factors can affect the survival and activity of the applied bacteria and consequently plant growth and phy- toremediation efficiency. The effect of inoculum density on the abundance and expression of alkune-degrading genes in the rhizosphere of plant vegetated in hydrocarbon-contaminated soil has been rarely observed. In this study, an alkane-degrading bacterium (Pantoea sp. strain BTRH79), at different inoculum densities (10^5 to 10^8 cells cm^-3 soil), was inoculated to ryegrass (Lolium perenne) vegetated in diesel-contaminated soil to find the optimum inoculum density needed for its efficient colonization and hydrocarbon degradation activity. Bacterial inoculation improved plant growth and hydrocarbon degradation. Maximum plant growth and hydrocarbon degra- dation were observed with the inoculum having the highest cell density (10^8 cells cm^-3 soil). Moreover, the inoculum with higher cell density exhibited more abundance and expression of alkane hydroxylase gene, CYP153. This study suggests that the inoculum density is one of the main factors that can affect bacterial colonization and activity during phytoremediation.