中西医结合治疗对痰瘀互结型非酒精性脂肪肝患者相关指标及中医证候积分的影响分析

分析中西医结合的治疗方案对痰瘀互结型非酒精性脂肪肝病情的影响。方法 纳入灵山县人民医院收治的38例痰瘀互结型非酒精性脂肪肝患者,研究时长为两年半,2020.1开始,2023.6结束,以交叉分组的方式分组,其中50%患者采用单一西药治疗(参照组,口服水飞蓟宾胶囊),另50%患者采用中西结合治疗(治疗组,口服水飞蓟宾胶囊以及中药脂肝消方),以肝功能指标、肝纤维化指标、中医证候积分、不良反应纳入对比项目,使用SPSS25.0处理数据。结果 治疗前,两组患者肝功能指标、肝纤维化指标、中医证候积分等数据统计学P>0.05,治疗后,两组肝功能指标、肝纤维化指标,脘腹胀满、胁肋刺痛或钝痛、纳呆厌油、形体肥胖等中医证候积分降低,而治疗组下降幅度更大,统计学P<0.05。治疗组的治疗有效率较之于参照组更高,统计学P<0.05。两组患者治疗过程中均未出现不良反应。结论 针对痰瘀互结型非酒精性脂肪肝患者采用水飞蓟宾+中药脂肝消方的中西结合治疗方案,可明显改善肝功能以及不良症状,用药安全性较好,有应用价值。

关系数据库中非结构型数据的处理

本文分析了非结构型数据的特点，探讨了关系数据库中对非结构型数据的处理方法，并对数据库的存取操作及应用范围进行了讨论。

应用点突变预测程序(SIFT)检查MLH1蛋白质中的结肠癌相关点突变

目的:分析MLH1蛋白质序列上的结肠癌相关点突变,并确定突变对蛋白质功能的影响程度。方法:通过SIFT(Sorting Intolerant From Tolerant)程序来评价和预测点突变对蛋白质功能的影响。结果:MLH1蛋白序列含有756个氨基酸,已确定的突变82个,其中和结肠癌相关的点突变有74个。23个结肠癌相关点突变属于严重等级,14个属于中度影响等级,剩下的37个点突变属于良性等级。结论:运用SIFT程序可迅速准确地预测p53蛋白质序列上的结肠癌相关点突变。

Application of discrete choice model in trip mode structure forecast:a case study of Bengbu

In order to find the main factors that influence the urban traffic structure,a relational model between the travelers＇ characteristics and the trip mode choice is built.The data of urban residents＇ characteristics are obtained from statistical data,while the trip mode split data is collected through a trip survey in Bengbu.In addition,the discrete choice model is adopted to build the functional relationship between the mode choice and the travelers＇ personal characteristics,as well as family characteristics and trip characteristics.The model shows that the relationship between the mode split and the personal,as well as family and trip characteristics is stable and changes little as the time changes.Deduced by the discrete model,the mode split result is relatively accurate and can be feasibly used for trip mode structure forecasts.Furthermore,the proposed model can also contribute to find the key influencing factors on trip mode choice,and restructure or optimize the urban trip mode structure.

A FVCOM-Based Unstructured Grid Wave, Current,Sediment Transport Model,I.Model Description and Validation

An effort was made to couple FVCOM (a three-dimensional (3D),unstructured grid,Finite Volume Coastal Ocean Model) and FVCOM-SWAVE (an unstructured grid,finite-volume surface wave model) for the study of nearshore ocean processes such as tides,circulation,storm surge,waves,sediment transport,and morphological evolution.The coupling between FVCOM and FVCOM-SWAVE was achieved through incorporating 3D radiation stress,wave-current-sediment-related bottom boundary layer,sea surface stress parameterizations,and morphology process.FVCOM also includes a 3D sediment transport module.With accurate fitting of irregular coastlines,the model provides a unique tool to study sediment dynamics in coastal ocean,estuaries,and wetlands where local geometries are characterized by inlets,islands,and intertidal marsh zones.The model was validated by two standard benchmark tests: 1) spectral waves approaching a mild sloping beach and 2) morphological changes of seabed in an idealized tidal inlet.In Test 1,model results were compared with both analytical solutions and laboratory experiments.A further comparison was also made with the structured grid Regional Ocean Model System (ROMS),which provides an insight into the performance of the two models with the same open boundary forcing.

Down-regulated expression of atypical PKC-binding domain deleted asip isoforms in human hepatocellular carcinomas

Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5’-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,353 aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 52.6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.

Effect of pile-cap connection on behavior of torsionally loaded pile groups

To evaluate the responses of fixed and pinned pile groups under torsion, a method is presented to analyze the nonlinear behavior of free-standing pile groups with rigid pile caps. The method is capable of simulating the nonlinear soil response in the near field usingp-y and r-θ curves, the far-field interactions through Mindlin＇s and Randolph＇s elastic solutions, and the coupling effect of lateral resistance on torsional resistance of the individual piles using an empirical factor. Based on comparisons of the solutions for fixed- and pinned-head, 1×2, 2×2, and 3×3 pile groups subjected to torsion, it was found that pile-cap connection significantly influences the torsional capacity of pile groups and the assignment of applied torques in the pile groups. In this study, the applied torques for the pinned-head pile groups are only 44%-64% of those for the corresponding fixed-head pile groups at a twist angle of 2^o. Such a difference is mainly due to the change of the lateral resistances of individual piles in the groups.

First report of a de novo germline mutation in the MLH1 gene

Hereditary non-polyposis colorectal carcinoma （HNPCC） is an autosomal dominant disorder associated with colorectal and endometrial cancer and a range of other tumor types. Germline mutations in the DNA mismatch repair （MMR） genes, particularly MLH1, MEH2, and MEH5, underlie this disorder. The vast majority of these HNPCC-associated mutations have been proven, or assumed, given the family history of cancer, to be transmitted through several generations. To the best of our knowledge, only a single case of a de novo germline MMR gene mutation （in MEH2） has been reported till now. Here, we report a patient with a de novo mutation in MLH1. We identified a MLH1 Q701X truncating mutation in the blood lymphocytes of a male who had been diagnosed with rectal cancer at the age of 35. His family history of cancer was negative for the first- and second-degree relatives. The mutation could not be detected in the patient＇s parents and sibling and paternity was confirmed with a set of highly polymorphic markers. Non-penetrance and small family size is the common explanation of verified negative family histories of cancer in patients with a germline MMR gene mutation. However, in addition to some cases explained by non-paternity, de novo germline mutations should be considered as a possible explanation as well. As guidelines that stress not to restrict MMR gene mutation testing to patients with a positive family history are more widely introduced, more cases of de novo MMR gene germline mutations may be revealed.

Three-Dimensional Structure of Optimal Nonlinear Excitation for Decadal Variability of the Thermohaline Circulation

The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results show that the optimal initial perturbations of temperature and salinity exciting the strongest decadal THC variations have similar structures: the perturbations are mainly in the northwestern basin at a depth ranging from 1500 to 3000 m. These temperature and salinity perturbations act as the optimal precursors for future modifications of the THC, highlighting the importance of observations in the northwestern basin to monitor the variations of temperature and salinity at depth. The decadal THC variation in the nonlinear model initialized by the optimal salinity perturbations is much stronger than that caused by the optimal temperature perturbations, indicating that salinity variations might play a relatively important role in exciting the decadal THC variability. Moreover, the decadal THC variations in the tangent linear and nonlinear models show remarkably different characteristics, suggesting the importance of nonlinear processes in the decadal variability of the THC.

The Partition of Unity Method for High-Order Finite Volume Schemes Using Radial Basis Functions Reconstruction

This paper introduces the use of partition of unity method for the development of a high order finite volume discretization scheme on unstructured grids for solving diffusion models based on partial differential equations.The unknown function and its gradient can be accurately reconstructed using high order optimal recovery based on radial basis functions.The methodology proposed is applied to the noise removal problem in functional surfaces and images.Numerical results demonstrate the effectiveness of the new numerical approach and provide experimental order of convergence.

Function of nonstructural 5A protein of genotype 2a in replication and infection of HCV with gene substitution

AIM:To explore the function of Nonstructural 5A(NS5A) protein of genotype 2a(JFH1)in the replication and infection of hepatitis C virus(HCV).METHODS:Intergenotypic chimera FL-J6JFH/J4NS5A was constructed by inserting NS5A gene from 1b stain HC-J4 by the overlapping polymerase chain reaction (PCR)method and the restriction enzyme reaction.In vitro RNA transcripts of chimera,prototype J6JFH and negative control J6JFH1(GND)were prepared and transfected into Huh-7.5 cells with liposomes.Immunofluorescence assay(IFA),fluorescence quantitative PCR and infection assay were performed to determine the protein expression and gene replication in Huh-7.5 cells.RESULTS:The HCV RNA levels in FL-J6JFH/J4NS5A chimera RNA transfected cells were significantly lower than the wild type at any indicated time point(2.58 ±5.97×106 vs 4.27±1.72×104,P=0.032).The maximal level of HCV RNA in chimera was 5.6±1.8× 104 GE/μg RNA at day 34 after transfection,while the wild type reached a peak level at day 13 which was 126 folds higher(70.65±14.11×105 vs 0.56±0.90 ×105,P=0.028).HCV proteins could also be detected by IFA in chimera-transfected cells with an obviously low level.Infection assay showed that FL-J6JFH/J4NS5A chimera could produce infectious virus particles,ranging from 10±5 ffu/mL to 78.3±23.6 ffu/mL,while that of FL-J6JFH1 ranged from 5.8±1.5×102 ffu/mL to 2.5±1.4×104 ffu/mL.CONCLUSION:JFH1 NS5A might play an important role in the robust replication of J6JFH1.The establishment of FL-J6JFH/J4NS5A provided a useful platform for studying the function of other proteins of HCV.

A model and simulation of cathode flooding and drying on unsteady proton exchange membrane fuel cell

A water balance has a significant impact on the overall system performance in proton exchange membrane fuel cell.An actual fuel cell application has a dynamic electrical load which means also dynamic electrical current.Therefore,since this electrical current is known,the water production from the fuel cell reaction is also able to be predicted.As long as the fuel cell water transportation model is provided,the present liquid water inside the porous medium is also able to be modeled.A model of the liquid water saturation level in a fuel cell in unsteady load condition was proposed.This model is a series of the water transportation model of water saturation level for the final output of proton exchange membrane(PEM) fuel cell to predict the flooding or drying of PEM fuel cell.The simulation of vehicle fuel cell in different dynamic load profiles and different inlet air conditions was done using this model.The simulation result shows that PEM fuel cell with different dynamic load profiles has different liquid water saturation level profiles.This means that a dynamic load fuel cell requires also a dynamic input air humidification.

Simultaneous optimization and control for polypropylene grade transition with two-layer hierarchical structure

In this paper,a two-layer hierarchical structure of optimization and control for polypropylene grade transition was raised to overcome process uncertain disturbances that led to the large deviation between the open-loop reference trajectory and the actual process.In the upper layer,the variant time scale based control vector parametric methods(VTS-CVP) was used for dynamic optimization of transition reference trajectory,while nonlinear model predictive controller(NMPC) based on closed-loop subspace and piece-wise linear(SSARX-PWL) model in the lower layer was tracking to the reference trajectory from the upper layer for overcoming high-frequency disturbances.Besides,mechanism about trajectory deviation detection and optimal trajectory updating online were introduced to ensure a smooth transition for the entire process.The proposed method was validated with the real data from an industrial double-loop propylene polymerization reaction process with developed dynamic mechanism mathematical model.

Interference Resistance of Pentamaran Ship Model With Asymmetric Outrigger Configurations

An experimental investigation is performed to assess the relation of interference performance on the total resistance of a pentamaran model advancing in calm water. For this motivation, the total drag of the ship is performed for several values of asymmetric outrigger configuration and hull separation, altering the Froude number in the range 0.3–0.9. Our results indicate that remarkable changes in resistance require notable changes in transverse distance values （hull separation） when wave interference may occur. In addition, there is no single configuration that consistently outperforms the other configurations across the entire speed range and the optimum interference factor -0.2 appears at a Froude number of 0.45 in S/L=0.33 with the outrigger outer position： asymmetric outboard for A3 configuration.

ANALYTIC STUDY ON POTENTIAL INSTABILITY AND SPIRAL STRUCTURE OF RAIN CLUSTERS

This paper presents a study on potential instability and spiral structure of unstable rain clusters.First,we develop a linearized non-axisymmetrical mathematic model for rain clusters in circular cylindrical coordinates and acquire its analytic solution.Second,we discuss the potential instability of non-axisymmetrical rain clusters.Finally,we conclude that spiral structures can exist in rain clusters.Our analysis indicates that potential instability occurs when humid stratification coefficient is less than zero.Unstable growth rate increases with the increase of the absolute value for humid stratification coefficient.The simpler the vertical structure of perturbation,the thicker the inversion layer;additionally,the smaller the radius of the rain clusters,the larger the unstable growth rate.Simulation results agree well with those from observation and forecast.The spiral structure simulated by our model is similar to a radar echo,suggesting that rain clusters with spiral structures can occur in the atmosphere.In addition,they are generally close to the model solution in this work.

Performance Prediction of Structured Packing Column for Cryogenic Air Separation with Hybrid Model

A detailed investigation of a thermodynamic process in a structured packing distillation column is of great impor- tance in prediction of process efficiency. In order to keep the simplicity of an equilibrium stage model and the accu- racy of a non-equilibrium stage model, a hybrid model is developed to predict the structured packing column in cryogenic air separation. A general solution process for the equilibrium stage model is developed to solve the set of equations of the hybrid model, in which a separation efficiency function is introduced to obtain the resulting tri-diagonal matrix and its solution by the Thomas algorithm. As an example, the algorithm is applied to analyze an upper column of a cryogenic air separation plant with the capacity of 17000 m3·h-1. Rigorous simulations are conducted using Aspen RATEFRAC module to validate the approach. The temperature and composition distributions are in a good agreement with the two methods. The effects of inlet/outlet position and flow rate on the temperature and composition distributions in the column are analyzed. The results demonstrate that the hybrid model and the solution algorithms are effective in analvzin~ the distillation process for a a cryogenic structured packing column.

Non-Linear Dependence in Oil Price Behavior

In this paper, the authors analyze the adequacy of GARCH-type models to analyze oil price behavior by applying two types of non-parametric tests, the Hinich portmanteau test for non-linear dependence and a frequency-dominant test of time reversibility, the reverse test based on the bispectrum, to explore the high-order spectrum properties of the Mexican oil price series. The results suggest strong evidence of a non-linear structure and time irreversibility. Therefore, it does not comply with the i.i.d （independent and identically distributed） property. The non-linear dependence, however, is not consistent throughout the sample period, as indicated by a windowed test, suggesting episodic nonlinear dependence. The results imply that GARCH models cannot capture the series structure.

Practical Model for Real Structure and Performance of Heavy Vehicles

In this paper, a new practical model for real heavy vehicle structure is developed to investigate dynamic responses under steering/acceleration or braking maneuvers. The generalized six DoFs （degrees-of-freedom） nonlinear vehicle model M1 including longitudinal, lateral, yaw, vertical, roll and pitch dynamics is validated using the measured data reported in different studies. This model takes the CG （center of gravity） of sprung mass, unsprung mass and total vehicle mass into account. Based on this model, the effects of the inertia parameters on the vehicle dynamic responses are investigated for more comprehensive assessments of the model structure. Another nonlinear vehicle model 342 derived from M1 which assumes that the vehicle has a single CG as reported in literature is also developed. The dynamic responses of the vehicle model Mj are compared with those of the model M2 to demonstrate the performance potential of the proposed nonlinear model. The results of dynamic responses with the nonlinear vehicle model MI suggest that the model could offer considerable potential in realizing enhanced ride and handling performance, as well as improved roll and pitch properties in a flexible manner.