一株非还原性二糖——海藻糖产生菌的筛选

报导了一株非还原性二糖———海藻糖产生菌的筛选过程。通过平板初筛和摇瓶产酶转化试验 ,经TLC及HPLC检测 ,确证得到一株海藻糖产生菌 ,转化率为 13.8% ,对该菌株形状的研究 。

树舌灵芝中分离得到非还原性二糖—海藻糖(英文)

Trehalose, a non-reducing disaccharide, was separated for the first time from Ganoderma applanatum fruit body. The structure of trehalose was determined by electrospray ionization mass spectrometric and NMR data. The content of trehalose was determined by improved anthrone-sulphuric acid colorimetric method and it was 0.48% of dried weight of G. applanatum fruit body. Mannitol was simultaneously obtained during separation of trehalose from G. applanatum.

蔗糖及其有关物质含量的HPLC-CAD分析研究

目的:建立蔗糖及其有关物质含量的HPLC-CAD分析测定法。方法:采用Waters XBridge Amide色谱柱(4.6 mm×250 mm,3.5μm),柱温为30℃;以乙腈-0.2%三乙胺水溶液(65∶35)为流动相,流速为1.0 mL·min^(-1);检测器为电喷雾检测器(CAD),雾化温度HIGH(50℃),幂率1.15,采样频率10 Hz,过滤常数5.0 s。结果:蔗糖与相邻杂质以及各杂质峰之间均达到基线分离;蔗糖质量浓度在4.95～99.08μg·mL^(-1)及0.30～0.80 mg·mL^(-1)范围内线性关系良好(r>0.999),可分别用于有关物质检查和含量测定;检测下限为1.49μg·mL^(-1),定量下限为4.95μg·mL^(-1);进样精密度RSD为0.31%;重复性RSD为0.61%。4个批次的蔗糖样品含量均在99.09%～100.48%之间,有关物质含量在0.15%～0.25%之间。结论:所建方法专属、灵敏、耐用,操作简单,具有很好的应用前景。