According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning...According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning the complete coding region of SENV-D variant gene were amplified by semi-nested PCRThe amplified fragments were cloned and sequencedThe nucleotide sequence homology of this Chinese strain with SENV-D(AX025730),SENV-D(AB059352) and TTV(AB028668) were 90%,88% and 91% respectivelyThe protein encoded by ORF1 has two putative conserved sequence motifs relating to the replication of the virus and,besides,a conserved ATP/GTP-binding motif and several highly conserved protein kinase phosphorylation展开更多
文摘According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning the complete coding region of SENV-D variant gene were amplified by semi-nested PCRThe amplified fragments were cloned and sequencedThe nucleotide sequence homology of this Chinese strain with SENV-D(AX025730),SENV-D(AB059352) and TTV(AB028668) were 90%,88% and 91% respectivelyThe protein encoded by ORF1 has two putative conserved sequence motifs relating to the replication of the virus and,besides,a conserved ATP/GTP-binding motif and several highly conserved protein kinase phosphorylation
