用“皮肤生物钟”规律指导皮肤美容护理

皮肤是人体最大的器官,其结构是相对稳定的,但皮肤的功能和活力在一天中是随人体功能的不断变化而出现规律性变化。这种规律性变化是人体生物钟的一个重要组成部分,并具有一定特点,笔者暂且称为"皮肤生物钟"。皮肤美容和护理若能按照"皮肤生物钟"来制定护理计划,必将产生意想不到的美容效果。

Facial pain induces the alteration of transient receptor potential vanilloid receptor 1 expression in rat trigeminal ganglion

Objective To investigate the involvement of transient receptor potential vanilloid receptor 1 （TRPV1） in the facial inflammatory pain in relation to thermal hyperalgesia and cold pain sensation. Methods Facial inflammatory pain model was developed by subcutaneous injection of turpentine oil （TO） into rat facial area. Head withdrawal thermal latency （HWTL） and head withdrawal cold latency （HWCL） were measured once a day for 21 d after TO treatment using thermal and cold measurement apparatus. The immunohistochemical staining, cell-size frequency analysis and the survey of average optical density （OD） value were used to observe the changes of TRPV1 expression in the neurons of the trigeminal ganglion （TG）, peripheral nerve fibers in the vibrissal pad, and central projection processes in the trigeminal sensory nuclei caudalis （Vc） on day 3, 5, 7, 14, and 21 after TO injection. Results HWTL and HWCL decreased significantly from day 1 to day 14 after TO injection with the lowest value on day 5 and day 3, respectively, and both recovered on day 21. The number of TRPV1-labeled neurons increased remarkably from day 1 to day 14 with a peak on day 7, and returned back to the normal level on day 21. In control rats, only small and medium-sized TG neurons were immunoreactive （IR） to TRPV1, and the TRPV1-IR terminals were abundant in both the vibrissal pad and the Vc. Within 2 weeks of inflammation, the expression of TRPV1 in small and medium-sized TG neurons increased obviously. Also the TRPV1 stained terminals and fibers appeared more frequent and denser in both the vibrissal pad skin and throughout laminae Ⅰ and the outer zone of laminae Ⅱ （Ⅱo） of Vc. Conclusion Facial inflammatory pain could induce hyperalgesia to noxious heat and cold stimuli, and result in increase of the numbers of TRPV1 positive TG neurons and the peripheral and central terminals of TG. These results suggest that the phenotypic changes of TRPV1 expression in small and medium-sized TG neurons and terminals might play an important role in the development and maintenance of TO-induced inflammatory thermal hyperalgesia and cold pain sensation.

OBSERVATION ON CLINICAL THERAPEUTIC EFFECTS OF SCALP ACUPUNCTURE FOR FACIAL SPASM

Objective; To observe clinical therapeutic effect of scalp acupuncture in the treatment of facial spasm. Methods： Sixty-five cases of facial spasm were randomly divided into control group （n = 31 ） and treatment group（n = 34）. Patients of the treatment group were treated with penetrative acupuncture from Qienshencong（前神聪 Extra） to Xuanli（悬厘 GB 6）, Baihui（百会 GV 20） to Qubin（曲鬓 GB 7）, etc., and those of control group treated with acupuncture of Taiyang（太阳 EX-HN 5）, Yengbai（阳白 GB 14）, Quanliao（颧髎 SI 18） and Xiaguan（下关 ST 7）, etc. once daily, 20 sessions altogether. Results. After treatment, of the 31 cases and 34 cases in control and treatment groups, 5 and 12 were cured, 8 and 13 had remarkable improvement, 11 and 8 had improvement, 7 and 1 failed, with the total effective rates being 77.42% and 97.06% respectively; and the therapeutic elfact of treatment group was significantly superior to that of control group（P〈0.01）. Conclusion; Scalp acupurcture has an obvious therapeutic effect for facial spasm.

Olfactory ensheathing cells promote nerve regeneration and functional recovery after facial nerve defects

Olfactory ensheathing cells from the olfactory bulb and olfactory mucosa have been tbund to increase axonal sprouting and pathfinding and promote the recovery of vibrissae motor performance in facial nerve transection injured rats. However, it is not yet clear whether olfactory ensheathing cells promote the reparation of facial nerve defects in rats. In this study, a collagen sponge and silicone tube neural conduit was implanted into the 6-mm defect of the buccal branch of the facial nerve in adult rats. Olfactory ensheathing cells isolated from the olfactory bulb of newborn Sprague-Dawley rats were injected into the neural conduits connecting the ends of tile broken nerves, the morphology and function of the regenerated nerves were compared between the rats implanted with olfactory ensheathing cells with the rats injected with saline. Facial paralysis was assessed. Nerve electrography was used to measure facial nerve-induced action potentials. Visual inspection, anatomical microscopy and hematoxylin-eosin staining were used to assess the histomorphology around the trans planted neural conduit and the morphology of the regenerated nerve. Using fluorogold retrograde tracing, toluidine blue staining and lead uranyl acetate staining, we also measured the number of neurons in the anterior exterior lateral f：acial nerve motor nucleus, the number of myelinated nerve fibers, and nerve fiber diameter and myelin sheath thickness, respectively. After surgery, olfactory ensheathing cells de- creased facial paralysis and the latency of the facial nerve-induced action potentials. There were no differences in the general morphology of the regenerating nerves between the rats implanted with olfactory ensheathing cells and the rats injected with saline. Between-group results showed that olfactory ensheathing cell treatment increased the number of regenerated neurons, improved nerve fiber morphology, and increased the number of myelinated nerve fibers, nerve fiber diameter, and myelin sheath thickness. In conclusion, implantation of olfactory ensheathing cells can promote regeneration and functional recovery after facial nerve damage in rats.

Stuck with the Sur-face： An Ethical Approach to Facial Disfigurement in Willa Cather＇s ＂The Profile＂

The human face consists of three dimensions： （1） the visible, static sur-face, （2） the mobile dimension, which communicates thoughts, emotions, and states of mind, and （3） the invisible dimension, the face aspars-pro-toto for the whole person who owns the face. In this latter sense, the human face is at the center of Emmanuel Levinas＇ theory of ethics： We encounter each other face-to-face, he argues, and it is the other＇s face that calls for my ethical response to the holiness of his/her wholeness and essence. The primary ethical imperative is： ＂Thou shalt not kill.＂ When we encounter a facially disfigured person, we usually fail to respond ethically because we concentrate on the disfigurement. The second dimension, as a result of the disfigurement, is often impaired and thus incapable of communicating in such a way that we can ＂read＂ the face. With our gaze fixed on the disfigured part of the face, we lose sight of the person as a whole. Metaphorically speaking, we could even say that we ＂kill＂ the person. Levinas conceives of the other＇s face not in its plastic manifestation. But the encounter with the other does at first take place as embodiment. According to Merleau-Ponty, this encounter is by definition interactive and dialogical. In Willa Cather＇s short story ＂The Profile,＂ young beautiful Virginia marries a portrait painter after he has finished her portrait in profile. The left side of her face is severely disfigured because of a burn. Her husband is waiting impatiently for her to talk about ＂it＂ and the suffering it must cause her in order to ＂heal＂ her with his love, but she behaves as if it did not exist and even likes to dress extravagantly. In the following analysis of the short story, it will be illustrated how the husband fails to respond to his wife＇s face＇s invitation to interact with it and get in touch with the wholeness of her person that it stands for. Instead, he reduces her to the disfigurement. When he finally names the scar, he metaphorically ＂kills＂ her, as well as their marriage.

CHARACTERISTIC OF FACIAL MUSCLE AND EFFERENT NERVE ON PHYSIOLOGY AND ANATOMY

Objective.To study the speciality of orbicularis oculi muscles, nerves and oris muscles, nerves and mechanism of difference of faclal muscular injury in facial paralysis deeply.Methods. The conductive velocity of the efferent nerves of orbicularis oculi and oris muscles of the health human beings and guinea pigs is measured with electromyogram (EMG) apparatus.Results. The conductive velocity of orbicularis oculi nerve is quicker and oris muscles are controlled by facial nerves on both sides. Measuring the threshold of Strength-Duration (S-D) curves of the motor point of orbicularis oculi and oris muscles shows the threshold of the former is lower. Measuring the diameter of orbicularis oculi and oris nerve fibers on the guinea pigs and rabbits shows the diameter of orbicularis oculi nerve fiber is bigger. The area of secondary synapse space of orbicularis oculi motor end plate is larger than that of oris under scanning electromicroscope.Conclusions. Orbicularis oculi muscles,orbicularis oculi nerves and oris muscles,oris nerves all have their own characteristic on physiology and anatomy. It elucidated the mechanism that orbicularis oculi muscle is easy to be injuried.

Mechanism of action of two insect toxins huwentoxin-Ⅲ and hainantoxin-Ⅵ on voltage-gated sodium channels

Selenocosmia huwena and Selenocosmia hainana are two tarantula species found in southern China.Their venoms contain abundant peptide toxins.Two new neurotoxic peptides,huwentoxin-Ⅲ(HWTX-Ⅲ) and hainantoxin-VI(HNTX-VI),were obtained from the venom using ion-exchange chromatography and reverse-phase high performance liquid chromatography(RP-HPLC).The mechanism of action of HWTX-Ⅲ and HNTX-VI on insect neuronal voltage-gated sodium channels(VGSCs) was studied via whole-cell patch clamp techniques.In a fashion similar to δ-atracotoxins,HNTX-VI can induce a slowdown of current inactivation of the VGSC and reduction in the peak of Na+ current in cockroach dorsal unpaired median(DUM) neurons.Meanwhile,10 μmol/L HNTX-IV caused a positive shift of steady-state inactivation of sodium channel.HWTX-ⅡI inhibited VGSCs on DUM neurons(concentration of toxin at half-maximal inhibition(IC50)≈1.106 μmol/L) in a way much similar to tetrodotoxin(TTX).HWTX-Ⅲ had no effect on the kinetics of activation and inactivation.The shift in the steady-state inactivation curve was distinct from other depressant spider toxins.The diverse effect and the mechanism of action of the two insect toxins illustrate the diverse biological activities of spider toxins and provide a fresh theoretical foundation to design and develop novel insecticides.