A new method was used to preparing genomic DNA from Microbacterium sp.quickly and efficiently.DNA quantity and purity was measured by UV absorbance.Integrity of the genomic DNA was tested by agarose gel eletrophoresis...A new method was used to preparing genomic DNA from Microbacterium sp.quickly and efficiently.DNA quantity and purity was measured by UV absorbance.Integrity of the genomic DNA was tested by agarose gel eletrophoresis.The DNA prepared by this method was sufficiently pure for PCR.This method saves time and cost,practices easily as well.展开更多
Purpose: To determine the frequency of histologically verified lesions of the lacrimal drainage system in Denmark between the years 1910 and 1999. Furthermore , to correlate the clinical diagnosis with the pathology d...Purpose: To determine the frequency of histologically verified lesions of the lacrimal drainage system in Denmark between the years 1910 and 1999. Furthermore , to correlate the clinical diagnosis with the pathology diagnosis. Methods: Ret rospective review of all pathology reports from 1910 to 1999 in the files of the Eye Pathology Institute, University of Copenhagen, describing a lesion of the l acrimal drainage system. In addition, a retrospective review of all reports desc ribing a lesion of the lacrimal drainage system from the Danish Pathology Databa se. All specimens were re-evaluated, except in cases with a primary diagnosis o f dacryocystitis. In these cases a sample of 25%was re-evaluated. Results: A t otal of 643 lesions were collected. Dacryocystitis was the most frequent lesion, constituting 508 cases (79%). The remaining cases were diagnosed as dacryolith iasis (62 cases; 7.9%), tumour (29 cases; 4.5%), trauma (19 cases; 3.0%), con genital malformation (nine cases; 1.4%), canaliculitis (eight cases; 1.2%) and granulomatous inflammation (eight cases; 1.2%). Seventeen tumours were maligna nt, of which B-cell lymphoma was the most common (six cases). In 0.6%of cases with a clinical diagnosis of dacryocystitis/lithiasis a non-suspected malignant tumour was diagnosed. Micro-organisms were uncommon in dacryocystitis (9%) bu t frequent in cases of dacryolithiasis (87%). Conclusion: Dacryocystitis was by far the most frequent lesion of the lacrimal drainage system referred for histo pathological evaluation. Dacryolithiasis was often associated with micro-organi sms, especially Gram positive rods. Histopathology is necessary to confirm suspe cted tumours, more than half of which were inflammatory lesions, and to detect t umours that sometimes masquerade as inflammation.展开更多
文摘A new method was used to preparing genomic DNA from Microbacterium sp.quickly and efficiently.DNA quantity and purity was measured by UV absorbance.Integrity of the genomic DNA was tested by agarose gel eletrophoresis.The DNA prepared by this method was sufficiently pure for PCR.This method saves time and cost,practices easily as well.
文摘Purpose: To determine the frequency of histologically verified lesions of the lacrimal drainage system in Denmark between the years 1910 and 1999. Furthermore , to correlate the clinical diagnosis with the pathology diagnosis. Methods: Ret rospective review of all pathology reports from 1910 to 1999 in the files of the Eye Pathology Institute, University of Copenhagen, describing a lesion of the l acrimal drainage system. In addition, a retrospective review of all reports desc ribing a lesion of the lacrimal drainage system from the Danish Pathology Databa se. All specimens were re-evaluated, except in cases with a primary diagnosis o f dacryocystitis. In these cases a sample of 25%was re-evaluated. Results: A t otal of 643 lesions were collected. Dacryocystitis was the most frequent lesion, constituting 508 cases (79%). The remaining cases were diagnosed as dacryolith iasis (62 cases; 7.9%), tumour (29 cases; 4.5%), trauma (19 cases; 3.0%), con genital malformation (nine cases; 1.4%), canaliculitis (eight cases; 1.2%) and granulomatous inflammation (eight cases; 1.2%). Seventeen tumours were maligna nt, of which B-cell lymphoma was the most common (six cases). In 0.6%of cases with a clinical diagnosis of dacryocystitis/lithiasis a non-suspected malignant tumour was diagnosed. Micro-organisms were uncommon in dacryocystitis (9%) bu t frequent in cases of dacryolithiasis (87%). Conclusion: Dacryocystitis was by far the most frequent lesion of the lacrimal drainage system referred for histo pathological evaluation. Dacryolithiasis was often associated with micro-organi sms, especially Gram positive rods. Histopathology is necessary to confirm suspe cted tumours, more than half of which were inflammatory lesions, and to detect t umours that sometimes masquerade as inflammation.
