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瑞氏和革氏染色法查滴虫、霉菌及淋菌的方法学探讨及质控 被引量:15
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作者 安邦权 凌晓午 +4 位作者 胡玫 姜鹏飞 何平 杨飞涛 刘广宣 《陕西医学检验》 1998年第3期33-33,共1页
将320例白带标本制成涂片后,每张涂片一半瑞氏染色一半革氏染色寻找滴虫、霉菌及淋菌,同时观察微生物象与异常细胞,并与悬浮法查滴虫、霉菌相比较.结果显示染色法查滴虫和霉菌的阳性检出率为40.9%和34.0%,明显高于悬浮法查滴... 将320例白带标本制成涂片后,每张涂片一半瑞氏染色一半革氏染色寻找滴虫、霉菌及淋菌,同时观察微生物象与异常细胞,并与悬浮法查滴虫、霉菌相比较.结果显示染色法查滴虫和霉菌的阳性检出率为40.9%和34.0%,明显高于悬浮法查滴虫和霉菌的29.8%和21.3%的阳性率(P<0.05和P<0.01).杂色法具有不受保温措施限制,医师涂片可保证标本质量,病原体被杀死而降低环境污染,尤其涂片可短期保存以便复查而建立质控措施等优点,可取代悬浮法推广应用. 展开更多
关键词 快速瑞染色 革氏染色 白带常规 滴虫 霉菌
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Selective inactivation of Gram-positive bacteria in vitro and in vivo through metabolic labelling 被引量:1
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作者 Tao Feng Hui Lu +6 位作者 Xiaoting Ye Chaofan Nie Jianhong Zhang Luofeng Yu Haoyu Jin Peng Li Wei Huang 《Science China Materials》 SCIE EI CAS CSCD 2022年第1期237-245,共9页
Bacterial infections are grave threats to human health,particularly those caused by the most common Grampositive bacteria.The massive administration of broad-spectrum antibiotics to treat various bacterial infections ... Bacterial infections are grave threats to human health,particularly those caused by the most common Grampositive bacteria.The massive administration of broad-spectrum antibiotics to treat various bacterial infections has led to the evolution and spread of drug resistance.As a universal antimicrobial technique unapt to induce drug resistance,photothermal therapy(PTT)is attracting extensive attention in recent years.However,its unspecific killing capability and side effects towards adjacent mammalian cells severely impede the practical applications.Herein,we proposed a metabolic engineering strategy to selectively inactivate Gram-positive bacteria by PTT.A bioorthogonal photothermal agent was prepared by the conjugation of IR-780 iodide and dibenzocyclooctyne(IR780-DBCO).Upon pre-metabolizing with 3-azido-D-alanine,Gram-positive bacteria rather than Gramnegative ones,such as Staphylococcus aureus and vancomycinresistant Enterococcus faecalis(VRE),could be specifically tied up by the explosive IR780-DBCO via copper-free click chemistry.Thereafter,they spontaneously detonated under 15 min near-infrared light irradiation and inactivated nearly 100% Gram-positive bacteria in vitro.Moreover,superbug VRE-induced infection was significantly inhibited by this approach in a mouse skin wound model.This metabolic labelling-based photothermal ablation strategy specific to Gram-positive microbes would stimulate the development of precise antibacterial candidates for preclinical applications. 展开更多
关键词 Gram-positive bacteria selective inactivation photothermal agent metabolic labelling wound infection
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