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大豆顶芽和子叶节组织培养再生成苗的研究 被引量:1
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作者 王慧中 李安生 邵启全 《江西农业大学学报》 CAS CSCD 1991年第4期352-356,共5页
以大豆品种小黑豆的顶芽作外植体,研究了激素、基本培养基对愈伤组织诱导及植株再生的影响.B_5+2,4-D0.5mg/L+KT0.1mg/L 作诱导培养基,愈伤组织诱导率达92.4%;愈伤组织在 MS+6-BA1mg/L+KT0.5mg/L+ZT0.5mg/L 的培养基上,分化频率47.2%.... 以大豆品种小黑豆的顶芽作外植体,研究了激素、基本培养基对愈伤组织诱导及植株再生的影响.B_5+2,4-D0.5mg/L+KT0.1mg/L 作诱导培养基,愈伤组织诱导率达92.4%;愈伤组织在 MS+6-BA1mg/L+KT0.5mg/L+ZT0.5mg/L 的培养基上,分化频率47.2%.以子叶节为外植体,通过附加外源的6-BA 刺激子叶节处潜在的分生细胞,使其不断分裂,结果子叶节膨大增粗.切下子叶节培养,结果每个子叶节最高能分化出11棵正常的苗. 展开更多
关键词 大豆 顶芹 子叶 组织培养 再生苗
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Essential Oil Composition of Endemic Pimpinella tunceliana YiId. from Turkey for the First Time
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作者 Omer Kilic Sinasi Yildirimli 《Journal of Agricultural Science and Technology(A)》 2015年第9期732-737,共6页
The objective of this study was to examine the essential oil ofPimpinella tunceliana Yild. growing wild in the Eastern part of Turkey for the first time. Obtained results were discussed with the genus patterns and nat... The objective of this study was to examine the essential oil ofPimpinella tunceliana Yild. growing wild in the Eastern part of Turkey for the first time. Obtained results were discussed with the genus patterns and natural products. The essential oil of P. tunceliana was analyzed by head space-solid phase microextraction (HS-SPME)/gas chromatography-mass spectrometry (GC-MS) technique, and 50 compounds were identified, representing 96.3% of the oil. The main constituents of P. tunceliana were β-caryophyllene (13.9%), β-bisabolene (10.4%), anethole (9.3%), sabinene (7.5%), limonene (6.9%) and germacrene D (6.8%). In this study, essential oil contents of P. tunceliana that is a new and endemic species was studied for the first time, and important results were presented in respect to the composition of the oil of this new species of Pimpinella. 展开更多
关键词 P. tunceliana essential oil HS-SPME/GC-MS.
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