Based on seismic and drilling data, we calculated tectonic subsidence amounts and rates of the Wan'an Basin by backstripping. The genetic mechanism and syn-rifting process of the basin were analyzed in combination...Based on seismic and drilling data, we calculated tectonic subsidence amounts and rates of the Wan'an Basin by backstripping. The genetic mechanism and syn-rifting process of the basin were analyzed in combination with the regional geological setting. The results reveal that the basin syn-rifted in the Eocene and early Miocene under the control of the dextral strike-slip Wan'an Fault Zone. The transtensional/ extentional stresses along this fault zone may be attributed to seafloor spreading of the South China Sea (SCS) in multiple episodes. Extensive basal faults and some small initial rifts in the early Paleogene can be related to southeastward extrusion and clockwise rotation of the Indochina Block. During the Oligocene, the nearly N-S directed spreading of the SCS derived the transtensional stresses in a roughly NW-SE orientation. The basin subsided rapidly in the middle and north to form two major subsidence centers. In the early Miocene, the SCS spread again in a nearly NW-SE direction, resulting in rapid subsidence in the southern basin continuous extending until the period ～16.3 Ma.展开更多
Objective： Fruit of Phyllanthus emblica Linn. （PE） is widely consumed as a functional food and used as a folk medicine due to its remarkable nutritional and pharmacological effects. Mitomycin C （MMC） and cisplat...Objective： Fruit of Phyllanthus emblica Linn. （PE） is widely consumed as a functional food and used as a folk medicine due to its remarkable nutritional and pharmacological effects. Mitomycin C （MMC） and cisplatin （cDDP） are the most widely used forms of chemotherapeutic drug, but their clinical use is limited by their genotoxicity to normal cells. We aimed to determine whether PE has potential to reduce the genotoxicity, while improving the anticancer effect, of MMC and cDDP. Methods： Cell proliferation was evaluated using the trypan blue exclusion assay and colony-forming assay. Genomic instability （GIN） was measured using the cytokinesis-block micronucieus assay. Results： Co-treatment （72 h） with PE at 20-320 μg/ml significantly enhanced the efficacy of MMC （0.05 μg/ml） and cDDP （1 μg/ml） against Colo205 colorectal cancer cells （P〈0.05）, and at 80-320 μg/ml significantly decreased MMC- and cDDP-induced GIN and multinucleation in normal colonic NCM460 cells （P〈0.05）. PE significantly decreased the mitotic index （P〈0.01）, blocked mitotic progression （P〈0.05）, and promoted apoptosis （P〈0.01） in MMC- and cDDP-treated NCM460 cells, suggesting that PE-mediated inhibition of mitosis and induction of apoptosis may limit the division and survival of highly damaged cells. Also, PE was found to inhibit the clonal expansion of MMC- and cDDP-treated NCM460 cells （P〈0.05） and decrease the heterogeneity of the surviving clones. Conclusions： PE potentiates the anticancer efficacy of MMC and cDDP, while preventing their genotoxicity and inhibiting clonal expansions of unstable genomes in normal cells. These data suggest that PE has the potential to reduce the risk of secondary cancers induced by chemotherapeutics.展开更多
基金Supported by the National Basic Research Program of China (973 Program) (No. 2009CB219406)the National Natural Science Foundation of China (No. 40930845)
文摘Based on seismic and drilling data, we calculated tectonic subsidence amounts and rates of the Wan'an Basin by backstripping. The genetic mechanism and syn-rifting process of the basin were analyzed in combination with the regional geological setting. The results reveal that the basin syn-rifted in the Eocene and early Miocene under the control of the dextral strike-slip Wan'an Fault Zone. The transtensional/ extentional stresses along this fault zone may be attributed to seafloor spreading of the South China Sea (SCS) in multiple episodes. Extensive basal faults and some small initial rifts in the early Paleogene can be related to southeastward extrusion and clockwise rotation of the Indochina Block. During the Oligocene, the nearly N-S directed spreading of the SCS derived the transtensional stresses in a roughly NW-SE orientation. The basin subsided rapidly in the middle and north to form two major subsidence centers. In the early Miocene, the SCS spread again in a nearly NW-SE direction, resulting in rapid subsidence in the southern basin continuous extending until the period ～16.3 Ma.
基金Project supported by the National Natural Science Foundation of China(Nos.31260268 and 31560307)
文摘Objective： Fruit of Phyllanthus emblica Linn. （PE） is widely consumed as a functional food and used as a folk medicine due to its remarkable nutritional and pharmacological effects. Mitomycin C （MMC） and cisplatin （cDDP） are the most widely used forms of chemotherapeutic drug, but their clinical use is limited by their genotoxicity to normal cells. We aimed to determine whether PE has potential to reduce the genotoxicity, while improving the anticancer effect, of MMC and cDDP. Methods： Cell proliferation was evaluated using the trypan blue exclusion assay and colony-forming assay. Genomic instability （GIN） was measured using the cytokinesis-block micronucieus assay. Results： Co-treatment （72 h） with PE at 20-320 μg/ml significantly enhanced the efficacy of MMC （0.05 μg/ml） and cDDP （1 μg/ml） against Colo205 colorectal cancer cells （P〈0.05）, and at 80-320 μg/ml significantly decreased MMC- and cDDP-induced GIN and multinucleation in normal colonic NCM460 cells （P〈0.05）. PE significantly decreased the mitotic index （P〈0.01）, blocked mitotic progression （P〈0.05）, and promoted apoptosis （P〈0.01） in MMC- and cDDP-treated NCM460 cells, suggesting that PE-mediated inhibition of mitosis and induction of apoptosis may limit the division and survival of highly damaged cells. Also, PE was found to inhibit the clonal expansion of MMC- and cDDP-treated NCM460 cells （P〈0.05） and decrease the heterogeneity of the surviving clones. Conclusions： PE potentiates the anticancer efficacy of MMC and cDDP, while preventing their genotoxicity and inhibiting clonal expansions of unstable genomes in normal cells. These data suggest that PE has the potential to reduce the risk of secondary cancers induced by chemotherapeutics.
