为建立以HPLC-ELSD法测定顺铂脂质体中DSPE-PEG2000含量的方法,以Waters Sun Fire prep Silica(250 mm×4.6 mm,5μm)为分离柱(柱温25℃),甲醇-冰醋酸-三乙胺(500∶8∶2,V/V/V)为流动相(流速1 m L/min),蒸发光散射检测器检测(雾化温...为建立以HPLC-ELSD法测定顺铂脂质体中DSPE-PEG2000含量的方法,以Waters Sun Fire prep Silica(250 mm×4.6 mm,5μm)为分离柱(柱温25℃),甲醇-冰醋酸-三乙胺(500∶8∶2,V/V/V)为流动相(流速1 m L/min),蒸发光散射检测器检测(雾化温度50℃,蒸发温度100℃,气体流速1.6 m L/min).结果表明,DSPE-PEG2000在21.08421.6μg/m L浓度范围内线性关系良好(r=0.999 3),加样回收率为99.03%101.23%(RSD=1.08%,n=9).该法能简便、快速、准确地测定顺铂脂质体中DSPE-PEG2000含量,重复性良好.展开更多
Objective:The aim of the study was to compare the efficacy,side effect and influence on the survival rate of two chemotherapy regimens,paclitaxel liposome combined with platinum and paclitaxel combined with platinum,i...Objective:The aim of the study was to compare the efficacy,side effect and influence on the survival rate of two chemotherapy regimens,paclitaxel liposome combined with platinum and paclitaxel combined with platinum,in concurrent chemoradiotherapy for cervical carcinoma.Methods:The 162 cases with primary cervical carcinoma diagnosed between January 2008 and November 2009 in Jiangxi Maternal and Child Health Hospital(China) were enrolled in this randomized controlled trial.Seventy-one cases were included in paclitaxel group and 91 in paclitaxel liposome group.And the chemotherapy doses were as follows:paclitaxel liposome and paclitaxel 135 mg/m2;cisplatin 80 mg/m2 or carboplatin AUC 4-6;then repeated every 21 days for two or three times.Radical radiotherapy was given to both groups at the same time.Efficacy was evaluated according to the tumor regression six months later and follow-up was done consistently.Results:The overall response rates of paclitaxel group and paclitaxel liposome group were 90.1% and 89 % respectively(P > 0.05).The one year cumulative survival was 91.4% for paclitaxel group and 89.2% for paclitaxel liposome group(P > 0.05).The incidence rates of adverse effects such as rash,gastrointestinal toxicity,bone marrow suppression and muscle/joint pain in paclitaxel liposome group were much lower than those in paclitaxel group(P < 0.05),while there was no difference regarding hair loss,hepatic function damage,peripheral neuritis and other aspects(P > 0.05).Conclusion:Paclitaxel liposome plus platinum is a safe and effective method for staging IIa-IV cervical carcinomas.While the long-term efficacy should be further observed.展开更多
文摘为建立以HPLC-ELSD法测定顺铂脂质体中DSPE-PEG2000含量的方法,以Waters Sun Fire prep Silica(250 mm×4.6 mm,5μm)为分离柱(柱温25℃),甲醇-冰醋酸-三乙胺(500∶8∶2,V/V/V)为流动相(流速1 m L/min),蒸发光散射检测器检测(雾化温度50℃,蒸发温度100℃,气体流速1.6 m L/min).结果表明,DSPE-PEG2000在21.08421.6μg/m L浓度范围内线性关系良好(r=0.999 3),加样回收率为99.03%101.23%(RSD=1.08%,n=9).该法能简便、快速、准确地测定顺铂脂质体中DSPE-PEG2000含量,重复性良好.
文摘Objective:The aim of the study was to compare the efficacy,side effect and influence on the survival rate of two chemotherapy regimens,paclitaxel liposome combined with platinum and paclitaxel combined with platinum,in concurrent chemoradiotherapy for cervical carcinoma.Methods:The 162 cases with primary cervical carcinoma diagnosed between January 2008 and November 2009 in Jiangxi Maternal and Child Health Hospital(China) were enrolled in this randomized controlled trial.Seventy-one cases were included in paclitaxel group and 91 in paclitaxel liposome group.And the chemotherapy doses were as follows:paclitaxel liposome and paclitaxel 135 mg/m2;cisplatin 80 mg/m2 or carboplatin AUC 4-6;then repeated every 21 days for two or three times.Radical radiotherapy was given to both groups at the same time.Efficacy was evaluated according to the tumor regression six months later and follow-up was done consistently.Results:The overall response rates of paclitaxel group and paclitaxel liposome group were 90.1% and 89 % respectively(P > 0.05).The one year cumulative survival was 91.4% for paclitaxel group and 89.2% for paclitaxel liposome group(P > 0.05).The incidence rates of adverse effects such as rash,gastrointestinal toxicity,bone marrow suppression and muscle/joint pain in paclitaxel liposome group were much lower than those in paclitaxel group(P < 0.05),while there was no difference regarding hair loss,hepatic function damage,peripheral neuritis and other aspects(P > 0.05).Conclusion:Paclitaxel liposome plus platinum is a safe and effective method for staging IIa-IV cervical carcinomas.While the long-term efficacy should be further observed.
