Docosahexaenoic acid (DHA) is an integral component of neural cell membranes and is critical to the development and function of the CNS. A premature delivery interrupts normal placental supply of DHA such that the inf...Docosahexaenoic acid (DHA) is an integral component of neural cell membranes and is critical to the development and function of the CNS. A premature delivery interrupts normal placental supply of DHA such that the infant is dependent on the nature of the nutritional support offered. The most abundant omega-3 fatty acid in preterm formulas is αlinolenic acid(ALNA),the precursor of DHA. This project studied the absorption,β-oxidation and conversion of ALNA to DHA by pre-term infants ranging from 30-37 wk of corrected gestation. U-13C -ALNA was administered emulsified with a preterm formula to 20 well preterm infants on full enteral feeds. Enrichment of 13C in stool and as 13CO2 in breath was used to estimate absorption across the gut and partitioning toward β-oxidation respectively. Excretion of the administered dose of 13C in stool ranged from 2.0 to 26.2%; excretion decreased with increasing birth gestation. Appearance as 13CO2 on breath ranged from 7.6 to 19.0%. All infants synthesised eicosapentaenoic acid (EPA),docosapentaenoic acid (DPA) and DHA with the least mature having the highest cumulative plasma DHA. These results show considerable variation suggesting that the worst absorption of ALNA and the greatest production of DHA occur in infants born at the earliest gestation.展开更多
目的研究二十二碳六烯酸(DHA)联合5氟尿嘧啶(5-FU)对人肝癌细胞HepG2耐药相关蛋白ERK、JNK及p38的表达。方法 Western blot检测5-FU5μg/mL,或5-FU5μg/mL联合DHA30μg/mL作用HepG2细胞48h后ERK、JNK及p38蛋白表达。结果与单用5-FU组比...目的研究二十二碳六烯酸(DHA)联合5氟尿嘧啶(5-FU)对人肝癌细胞HepG2耐药相关蛋白ERK、JNK及p38的表达。方法 Western blot检测5-FU5μg/mL,或5-FU5μg/mL联合DHA30μg/mL作用HepG2细胞48h后ERK、JNK及p38蛋白表达。结果与单用5-FU组比较,5-FU联合DHA组对耐药相关蛋白的影响,p-ERK明显受到抑制,而p-JNK表达显著增加,p-p38表达无差别。结论 DHA对肝癌细胞耐药相关蛋白的调控可能通过抑制ERK、激活JNK信号通路来增强5-FU的细胞毒活动,发挥增敏化疗药物的作用。展开更多
文摘Docosahexaenoic acid (DHA) is an integral component of neural cell membranes and is critical to the development and function of the CNS. A premature delivery interrupts normal placental supply of DHA such that the infant is dependent on the nature of the nutritional support offered. The most abundant omega-3 fatty acid in preterm formulas is αlinolenic acid(ALNA),the precursor of DHA. This project studied the absorption,β-oxidation and conversion of ALNA to DHA by pre-term infants ranging from 30-37 wk of corrected gestation. U-13C -ALNA was administered emulsified with a preterm formula to 20 well preterm infants on full enteral feeds. Enrichment of 13C in stool and as 13CO2 in breath was used to estimate absorption across the gut and partitioning toward β-oxidation respectively. Excretion of the administered dose of 13C in stool ranged from 2.0 to 26.2%; excretion decreased with increasing birth gestation. Appearance as 13CO2 on breath ranged from 7.6 to 19.0%. All infants synthesised eicosapentaenoic acid (EPA),docosapentaenoic acid (DPA) and DHA with the least mature having the highest cumulative plasma DHA. These results show considerable variation suggesting that the worst absorption of ALNA and the greatest production of DHA occur in infants born at the earliest gestation.
文摘目的研究二十二碳六烯酸(DHA)联合5氟尿嘧啶(5-FU)对人肝癌细胞HepG2耐药相关蛋白ERK、JNK及p38的表达。方法 Western blot检测5-FU5μg/mL,或5-FU5μg/mL联合DHA30μg/mL作用HepG2细胞48h后ERK、JNK及p38蛋白表达。结果与单用5-FU组比较,5-FU联合DHA组对耐药相关蛋白的影响,p-ERK明显受到抑制,而p-JNK表达显著增加,p-p38表达无差别。结论 DHA对肝癌细胞耐药相关蛋白的调控可能通过抑制ERK、激活JNK信号通路来增强5-FU的细胞毒活动,发挥增敏化疗药物的作用。
基金supported by the Educational Science Foundation of Beijing (No. KM200510025009, KM200710025004)theNational Basic Research Development Program of China (No. 2006CB503807)
基金This work was supported by the National Natural Science Foundation of China(No.30370578),ScienceTechnology Foundation of Educational Office of Heilongjiang Province(No.10551174)Graduate Innovation Foundation of Harbin Medical University.
