目的探究孕妇孕期血清样本中血清十八碳一不饱和脂肪酸硬脂酰鞘氨醇(serum stearoyl sphingosine,C18∶1-Cer)和1-硬脂酰基-sn-甘油-3-磷酸胆碱(lysophosphatidyl choline,LPC18∶0)水平在预测孕妇患妊娠期糖尿病(gestational diabetes ...目的探究孕妇孕期血清样本中血清十八碳一不饱和脂肪酸硬脂酰鞘氨醇(serum stearoyl sphingosine,C18∶1-Cer)和1-硬脂酰基-sn-甘油-3-磷酸胆碱(lysophosphatidyl choline,LPC18∶0)水平在预测孕妇患妊娠期糖尿病(gestational diabetes mellitus,GDM)方面的价值。方法回顾性分析126例孕妇的临床资料和实验室指标,根据GDM诊断结果,将研究对象分为GDM组(n=66)和对照组(n=60)。采用质谱法检测研究对象孕早期和孕中期血清C18∶1-Cer和LPC18∶0水平,通过Logistic回归分析筛选出GDM的危险因素,采用受试者工作特征(receiver operating characteristic,ROC)曲线评估C18∶1-Cer,LPC188∶0和两者联合对GDM的预测价值。结果与对照组相比,GDM组血清C18∶1-Cer和LPC18∶0水平在孕早期(18.92±2.77ng/ml vs 23.47±4.18ng/ml,41.32±17.55ng/ml vs 88.08±16.02ng/ml)和孕中期(23.14±4.10ng/ml vs 18.76±4.05ng/ml,84.60±14.53ng/ml vs 40.50±17.79ng/ml)均显著升高,差异具有统计学意义(t=7.127,15.637;-5.984,2.174,均P<0.05)。C18∶1-Cer与空腹血糖(fasting plasma glucose,FPG)、空腹胰岛素(fasting plasma insulin,FPI)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)、糖化血红蛋白(glycated hemoglobin,HbA1c)和三酰甘油(triglyceride,TG)呈正相关(r=0.458,0.209,0.317,0.223,0.219,均P<0.05)。LPC18∶0与FPG,FPI,HOMA-IR,HbA1c,总胆固醇(total cholesterol,TC)和TG呈正相关(r=0.715,0.426,0.580,0.465,0.232,0.372,均P<0.05)。Logistic回归分析结果显示,C18∶1-Cer[OR(95%CI):1.522(1.136~.039),P<0.05]和LPC18∶0[OR(95%CI):1.198(1.102~1.302),P<0.001]是GDM的独立危险因素。ROC曲线分析结果显示,血清C18∶1-Cer,LPC18∶0和两指标联合的曲线下面积(area under the curve,AUC)分别为0.819,0.971和0.986,两者联合检测的预测效能优于单独检测。结论妊娠早期血清中的C18∶1-Cer和LPC18∶0与GDM的发生密切相关,C18∶1-Cer联合LPC18∶0对GDM早期诊断有一定的预测价值。展开更多
The similarities of The mass spectra of any two isomers (n and m) are represented by a similarity index [SI(n, m)]. The similarity index is calculated using a novel formula: SI(n, m) ={min[R(n, i), R(m, i)] / max[R(n,...The similarities of The mass spectra of any two isomers (n and m) are represented by a similarity index [SI(n, m)]. The similarity index is calculated using a novel formula: SI(n, m) ={min[R(n, i), R(m, i)] / max[R(n, j), R(m, i)]} 100 Where R(n, j) and R(m, j) are The intensity ratio of a fragment pair (j); j is an ordinal number which is arbitrarily assigned to a selected pair of fragments. The value of similarity index ranges from 0 to 100, when n = m, The SI(n, m) = 100. Similarity indices of mass spectra of isomeric tetradecadien-1-ols were calculated by The "min.-max." Fuzzy similarity analysis. The universality of The method was confirmed by The testing with mass spectra of ten isomers of tetradecadien-1-ols.展开更多
The selective epoxidation of 3,3-(1,2-ethanediyl-bisoxy)-5(10),9(11)-estradiene-17-α-(1-propynyl)-17-β-ol with Fe(Ⅱ)Pc/PhIO was investigated at 25 ℃? atmospheric pressure. With a molar ratio of Fe(Ⅱ)Pc∶compound...The selective epoxidation of 3,3-(1,2-ethanediyl-bisoxy)-5(10),9(11)-estradiene-17-α-(1-propynyl)-17-β-ol with Fe(Ⅱ)Pc/PhIO was investigated at 25 ℃? atmospheric pressure. With a molar ratio of Fe(Ⅱ)Pc∶compound(I)∶PhIO at 6.25∶50∶120, the conversion reaches 100%. The overall yield of the products 5α, 10α-epoxide(2) and 5β, 10β-epoxide(3)is 60%, and the molar ratio of 2 to 3 is {8.39∶}1. The product 5α, 10α-epoxide is identified by melting point and HPLC.展开更多
探索新化合物Z-十八碳-9-烯-丙磺酰胺(N15)对糖尿病认知功能障碍(DACD)小鼠学习记忆功能的作用及机制。采用链脲佐菌素(STZ)连续小剂量腹腔注射以及高脂高糖加STZ诱导2型糖尿病小鼠模型,N15(50和100mg·kg^(-1)·d^(-1))连续灌...探索新化合物Z-十八碳-9-烯-丙磺酰胺(N15)对糖尿病认知功能障碍(DACD)小鼠学习记忆功能的作用及机制。采用链脲佐菌素(STZ)连续小剂量腹腔注射以及高脂高糖加STZ诱导2型糖尿病小鼠模型,N15(50和100mg·kg^(-1)·d^(-1))连续灌胃给药6周,于给药末期进行跳台、避暗以及Morris水迷宫测试以评价小鼠学习记忆功能;对海马内葡萄糖和乳酸水平进行测定;通过realtimePCR测定海马内突触成长相关蛋白-43(GAP-43)、突触素(SYN)、脑源性生长因子(BDNF)和神经营养因子-3(NT-3)m RNA的表达。结果表明,N15可显著改善模型小鼠学习记忆能力,降低海马内葡萄糖和乳酸含量,显著上调海马内GAP-43、SYN、BDNF和NT-3 m RNA表达水平。上述结果表明,新型化合物N15具有改善糖尿病认知功能障碍的作用,其机制可能与增加海马内突触生长相关因子和神经营养因子表达相关。展开更多
文摘目的探究孕妇孕期血清样本中血清十八碳一不饱和脂肪酸硬脂酰鞘氨醇(serum stearoyl sphingosine,C18∶1-Cer)和1-硬脂酰基-sn-甘油-3-磷酸胆碱(lysophosphatidyl choline,LPC18∶0)水平在预测孕妇患妊娠期糖尿病(gestational diabetes mellitus,GDM)方面的价值。方法回顾性分析126例孕妇的临床资料和实验室指标,根据GDM诊断结果,将研究对象分为GDM组(n=66)和对照组(n=60)。采用质谱法检测研究对象孕早期和孕中期血清C18∶1-Cer和LPC18∶0水平,通过Logistic回归分析筛选出GDM的危险因素,采用受试者工作特征(receiver operating characteristic,ROC)曲线评估C18∶1-Cer,LPC188∶0和两者联合对GDM的预测价值。结果与对照组相比,GDM组血清C18∶1-Cer和LPC18∶0水平在孕早期(18.92±2.77ng/ml vs 23.47±4.18ng/ml,41.32±17.55ng/ml vs 88.08±16.02ng/ml)和孕中期(23.14±4.10ng/ml vs 18.76±4.05ng/ml,84.60±14.53ng/ml vs 40.50±17.79ng/ml)均显著升高,差异具有统计学意义(t=7.127,15.637;-5.984,2.174,均P<0.05)。C18∶1-Cer与空腹血糖(fasting plasma glucose,FPG)、空腹胰岛素(fasting plasma insulin,FPI)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)、糖化血红蛋白(glycated hemoglobin,HbA1c)和三酰甘油(triglyceride,TG)呈正相关(r=0.458,0.209,0.317,0.223,0.219,均P<0.05)。LPC18∶0与FPG,FPI,HOMA-IR,HbA1c,总胆固醇(total cholesterol,TC)和TG呈正相关(r=0.715,0.426,0.580,0.465,0.232,0.372,均P<0.05)。Logistic回归分析结果显示,C18∶1-Cer[OR(95%CI):1.522(1.136~.039),P<0.05]和LPC18∶0[OR(95%CI):1.198(1.102~1.302),P<0.001]是GDM的独立危险因素。ROC曲线分析结果显示,血清C18∶1-Cer,LPC18∶0和两指标联合的曲线下面积(area under the curve,AUC)分别为0.819,0.971和0.986,两者联合检测的预测效能优于单独检测。结论妊娠早期血清中的C18∶1-Cer和LPC18∶0与GDM的发生密切相关,C18∶1-Cer联合LPC18∶0对GDM早期诊断有一定的预测价值。
文摘The similarities of The mass spectra of any two isomers (n and m) are represented by a similarity index [SI(n, m)]. The similarity index is calculated using a novel formula: SI(n, m) ={min[R(n, i), R(m, i)] / max[R(n, j), R(m, i)]} 100 Where R(n, j) and R(m, j) are The intensity ratio of a fragment pair (j); j is an ordinal number which is arbitrarily assigned to a selected pair of fragments. The value of similarity index ranges from 0 to 100, when n = m, The SI(n, m) = 100. Similarity indices of mass spectra of isomeric tetradecadien-1-ols were calculated by The "min.-max." Fuzzy similarity analysis. The universality of The method was confirmed by The testing with mass spectra of ten isomers of tetradecadien-1-ols.
文摘The selective epoxidation of 3,3-(1,2-ethanediyl-bisoxy)-5(10),9(11)-estradiene-17-α-(1-propynyl)-17-β-ol with Fe(Ⅱ)Pc/PhIO was investigated at 25 ℃? atmospheric pressure. With a molar ratio of Fe(Ⅱ)Pc∶compound(I)∶PhIO at 6.25∶50∶120, the conversion reaches 100%. The overall yield of the products 5α, 10α-epoxide(2) and 5β, 10β-epoxide(3)is 60%, and the molar ratio of 2 to 3 is {8.39∶}1. The product 5α, 10α-epoxide is identified by melting point and HPLC.
文摘探索新化合物Z-十八碳-9-烯-丙磺酰胺(N15)对糖尿病认知功能障碍(DACD)小鼠学习记忆功能的作用及机制。采用链脲佐菌素(STZ)连续小剂量腹腔注射以及高脂高糖加STZ诱导2型糖尿病小鼠模型,N15(50和100mg·kg^(-1)·d^(-1))连续灌胃给药6周,于给药末期进行跳台、避暗以及Morris水迷宫测试以评价小鼠学习记忆功能;对海马内葡萄糖和乳酸水平进行测定;通过realtimePCR测定海马内突触成长相关蛋白-43(GAP-43)、突触素(SYN)、脑源性生长因子(BDNF)和神经营养因子-3(NT-3)m RNA的表达。结果表明,N15可显著改善模型小鼠学习记忆能力,降低海马内葡萄糖和乳酸含量,显著上调海马内GAP-43、SYN、BDNF和NT-3 m RNA表达水平。上述结果表明,新型化合物N15具有改善糖尿病认知功能障碍的作用,其机制可能与增加海马内突触生长相关因子和神经营养因子表达相关。
