冷激条件下预形成副溶血性弧菌生物被膜的发展变化

【目的】生物被膜可附着在食品或医药生物材料表面引起持续性的感染,而现今极少有关于温度变化对预形成生物被膜影响的研究。本文分析了冷激条件下副溶血性弧菌预形成生物被膜的发展变化。【方法】以改进的结晶紫染色法检测生物被膜总量,以改进的超声法和Lowry法量化胞外多糖和蛋白质,以RNA试剂盒提取并纯化生物被膜形成的相关基因。同时,激光共聚焦扫描显微镜直观显示了冷激条件下预形成生物被膜形态结构的变化,并深入分析了生物被膜结构的变化,以及生物被膜结构参数和基因转录的相关性。【结果】在冷激条件下,副溶血性弧菌生物被膜总量增加,同时,副溶血性弧菌预形成的生物被膜胞外多聚物的主要成分胞外多糖和蛋白质也逐渐增加,被膜形成相关鞭毛基因和毒力基因的转录活跃。生物被膜的平均厚度(MT)、平均扩散距离(ADD)、孔隙率(P)、生物被膜粗糙度(BR)和均匀性(H)在冷激过程中也发生了变化,同时这些参数之间存在显著相关性(P<0.01),而生物被膜结构参数与生物被膜相关基因转录的相关性较弱(P<0.05)。【结论】因此,4°C和10°C冷激不能完全抑制副溶血性弧菌预形成生物被膜的生长,风险评估人员在制定控制食源性感染风险的战略时应考虑到这一因素。

芍药根茎芽发育及更新规律的形态学研究

对芍药根茎性质及根茎芽发生、发育及成枝规律进行动态观察研究,为揭示芍药营养繁殖特性,提高营养繁殖效率提供理论指导。结果表明:芍药根茎是芍药的地下短缩茎,节间产生不定根,节上有芽,属于腋芽类型。根茎以根茎芽来实现每年更新生长,最终呈现合轴分枝形态。根茎芽的叶元呈现:鳞片-茎节-侧芽-叶-茎节-腋芽的依次发生模式,从第5枚鳞片原基开始叶元由交互对生向螺旋状着生状态转变,并一直持续到花瓣原基形成。叶片同株异型,叶原基呈向顶式发育,而花原基呈向基式发育。通过越冬状态下的侧芽的发育状态观察,初步确定了根茎的茎节范围,根茎顶部1~2枚根茎芽在第2年春天萌发成茎,其余根茎芽则随根茎的生长发育长期处于休眠状态。越冬状态下,顶芽顶端分生组织进入雌蕊原基分化期,顶部4~5枚腋芽进入花瓣原基分化期,而其余腋芽处于原基初始发生状态而生长停滞,从而形成"一茎多枝"。

Prediction of stiffener buckling in press bend forming of integral panels

In order to predict the buckling of stiffeners in the press bend forming of the integral panel,a method for solving the critical buckling load of the stiffeners in press bend forming process was proposed based on energy method,elastic-plastic mechanics and numerical analysis.Bend to buckle experiments were carried out on the designed press bend dies.It is found that the predicted results based on the proposed method agree well with the experimental results.With the proposed method,the buckling of the stiffeners in press bend forming of the aluminum alloy integral panels with high-stiffener can be predicted reasonably.

Theoretical prediction of forming limit diagram of AZ31 magnesium alloy sheet at warm temperatures

A theoretical prediction on forming limit diagram（FLD） of AZ31 magnesium alloy sheet was developed at warm temperatures based on the M-K theory. Two different yield criteria of von Mises and Hill＇48 were applied in this model. Mechanical properties of AZ31 magnesium alloy used in the prediction were obtained by uniaxial tensile tests and the Fields-Backofen equation was incorporated in the analysis. In addition, experimental FLDs of AZ31 were acquired by conducting rigid die swell test at different temperatures to verify the prediction. It is demonstrated from a comparison between the predicted and the experimental FLDs at 473 K and 523 K that the predicted results are influenced by the type of yield criterion used in the calculation, especially at lower temperatures. Furthermore, a better agreement between the predicted results and experimental data for AZ31 magnesium alloy sheet at warm temperatures was obtained when Hill＇48 yield criterion was applied.

Springback prediction for incremental sheet forming based on FEM-PSONN technology

In the incremental sheet forming （ISF） process, springback is a very important factor that affects the quality of parts. Predicting and controlling springback accurately is essential for the design of the toolpath for ISF. A three-dimensional elasto-plastic finite element model （FEM） was developed to simulate the process and the simulated results were compared with those from the experiment. The springback angle was found to be in accordance with the experimental result, proving the FEM to be effective. A coupled artificial neural networks （ANN） and finite element method technique was developed to simulate and predict springback responses to changes in the processing parameters. A particle swarm optimization （PSO） algorithm was used to optimize the weights and thresholds of the neural network model. The neural network was trained using available FEM simulation data. The results showed that a more accurate prediction of s!oringback can be acquired using the FEM-PSONN model.

新合成核素^(223)Np及其α衰变链上核素的半衰期计算

基于α衰变的两势方法理论模型,采用考虑了同位旋效应的唯象cosh型α-子核核势和考虑了壳效应及质子—中子相互作用的α预形成因子解析式系统地计算了最近新合成的短寿命核素223Np及其α衰变链上核素α的衰变半衰期.计算结果表明,采用考虑了壳效应及质子—中子相互作用的α预形成因子的α衰变半衰期的理论计算结果能更好地符合实验数据.这项工作可以作为将来实验和理论研究α衰变及核结构的参考.

Optimizing microstructure and mechanical properties of heat-treated Al-Zn-Mg-Cu alloy by indirect hot deformation technology

In this paper,the indirect thermal tensile experiments of 7075 aluminum alloy including the pre-deformation process at room temperature and the final heat tensile process were carried out,the plastic deformation behavior and forming limit of the material in the compound forming process were investigated considering three pre-deformation amounts 4%,9%,14%,two strain rates 0.001 s^(-1),0.01 s^(-1) and four forming temperatures 300℃,350℃,400℃,450℃.In the indirect hot forming process,the material is sensitive to the pre-deformation,strain rate,and forming temperature,when the strain rate is 0.01 s^(-1),the pre-deformation amount is 4%,and the forming temperature is 400℃,respectively,the maximum tensile deformation is 50 mm.Finally,taking the process in which the forming temperature is 450℃ as an example,according to the observation of the microstructure appearance of fracture,the fracture type in the hot forming process was judged as the ductile fracture.By analyzing the microstructure of the specimen treated with the quenching and artificial aging process,the eutectic T(AlZnMgCu)phase and α(Al)matrix formed a network of non-equilibrium alpha binary eutectic.

Numerical study on forming complex fitting body on end of integrated stainless steel pipe without welds

It is necessary to use the integrated stainless steel pipe having two fitting bodies without welds while train travelling at high speed. In order to form this type of integrated stainless steel pipe, the method of preforming combined finish forming process is developed. The preforming process is characterized by flaring combined upsetting for left fitting body which is like a flange, and is characterized by tube axial compressive process under die constraint for right fitting body which is like a double-wall pipe. The finite element simulations of the processes are carried out by software package DEFORM, and the results indicate that: 1) left or right fitting body can be formed by a two-step forming process without folding and under-filling defects; 2) by using two-step forming, strain and stress in left fitting body are larger than those in right fitting body, and deformation in right fitting body is more homogenous than the deformation in left fitting body; 3) two or more preforming steps may be needed for left fitting body considering the distributions of strain and stress.

Clinical analysis of primary anaplastic carcinoma of the small intestine

Primary anaplastic carcinoma is a rare variant of small intestinal cancer. Most reports of primary anaplastic carcinoma of the small intestine are isolated case reports, therefore the clinicopathological features, therapeutic management, and surgical outcome of this tumor type remain unclear. This review analyzes the available clinical characteristics of primary anaplastic carcinoma of the small intestine and investigates key differences from differentiated adenocarcinoma of the small intestine. A Medline search was performed using the keywords ＇small intestine＇ and ＇anaplastic carcinoma＇ or ＇undifferentiated carcinoma＇. Additional articles were obtained from references within the papers identified by the Medline search. The literature revealed a poor prognosis for patients who underwent surgical resection for anaplastic carcinoma of the small intestine, which gave a 3-year overall survival rate of 10.8% and a median survival time of 5.0 mo. The literature suggests that anaplastic carcinoma~ is markedly more aggressive than differentiated adenocarcinoma of the small intestine. Surgical resection with the aim of complete tumor removal provides the only beneficial therapeutic option for patients with anaplastic carcinoma of the small intestine, because chemotherapy and radiation therapy have no significant effect on the rate of survival. However, despite complete tumor resection, most patients with anaplastic carcinoma of the small intestine are at great risk of disease recurrence. Multicenter clinical trials are expected to provide additional therapeutic strategies and establish the efficacy of multimodality adjuvant therapy. This report also highlights the importance of a systematic diagnostic approach for anaplastic carcinoma of the small intestine.

Effect of Qi-protecting powder (Huqi San) on expression of c-jun, c-fos and c-myc in diethylnitrosamine-mediated hepatocarcinogenesis

To study the inhibitory effect of Huqi San （Qi- protecting powder） on rat prehepatocarcinoma induced by diethylinitrosamine （DEN） by analyzing the mutational activation of c-fos proto-oncogene and over-expression of c-jun and c-myc oncogenes. METHODS： A Solt-Farber two-step test model of prehepatocarcinoma was induced in rats by DEN and 2-acetylaminofluorene （AAF） to investigate the modifying effects of Huqi San on the expression of c-jun, c-fos and c-myc in DEN-mediated hepatocarcinogenesis. Huqi San was made of eight medicinal herbs containing glycoprival granules, in which each milliliter contains 0.38 g crude drugs. T-glutamy-transpeptidase-isoenzyme （T-GTase） was determined with histochemical methods. Level of 8-hydroxydeoxyguanosine （OHdG） formed in liver and c-jun, c-fos and c-myc proto-oncogenes were detected by immunohistochemical methods. RESULTS： The level of 8-OHdG, a mark of oxidative DNA damage, was significantly decreased in the liver of rats with prehepatocarcinoma induced by DEN who received 8 g/kg body weight or 4 g/kg body weight Huqi San before （1 wk） and after DEN exposure （4 wk）. Huqi San- treated rats showed a significant decrease in number of T-GT positive foci （P 〈 0.001, prevention group： 4.96-0.72 vs 29.46-2.17; large dose therapeutic group： 7.53-0.88 vs 29.46-2.17）. On the other hand, significant changes in expression of c-jun, c-fos and c-myc were found in Huqi San-treated rats. CONCLUSION： Activation of c-jun, c-fos and c-myc plays a crucial role in the pathogenesis of liver cancer.Huqi San can inhibit the over-expression of c-jun, c-fos and c-myc oncogenes and liver preneolastic lesions induced by DEN.

Formation Mechanism and Size Prediction of Bubble in Opposite-Flowing T-Shaped Microchannel

Bubble formation in an opposite-flowing T-shaped microchannel with 40 μm in depth and 120 μm in width was real-time visualized and investigated experimentally by means of a high speed camera. N2 bubbles were generated in glycerol-water mixtures with different concentrations of surfactant sodium dodecyl sulfate (SDS). And the images were captured by the high speed camera linked to a computer. Results indicated that the bubble formation process can be distinguished into three consecutive stages, i.e., expansion, collapse and pinching off. The bubble size decreases with the increase of liquid flow rate and viscosity of liquid phase as well as the decrease of gas flow rate. The surface tension of the liquid phase has no measurable influence on the bubble size. Moreover, a new approach to predicting the size of bubbles formed in the T-shaped microchannel is proposed. And the predicted values agree well with the experimental data.

Effects of pre-deformation mode and strain on creep aging bend-forming process of Al-Cu-Li alloy

The bending deformation method was adopted to characterize the creep deformation behavior of Al-Cu-Li alloy in the creep aging forming(CAF) process based on a series of CAF tests, and the evolution laws of its mechanical properties and microstructures under different pre-deformation conditions were studied. The results show that the bending creep strain characterization method can intuitively describe the creep variation. With the increase of the pre-deformation strain, the creep strain of the specimen firstly increases and then decreases. The increase of the pre-deformation strain can promote the course of aging precipitation, and improve the formed alloy’s tensile properties at room temperature, the Kahn tearing properties, and the fatigue propagation properties. Pre-rolled specimens produce a slightly weaker work hardening than pre-stretched specimens, but they also create a stronger aging-strengthening effect;thus the strength, toughness and damage performance can be improved to some extent. Among all the types of specimens, the specimen with 3% rolling after CAF treatment has the best comprehensive mechanical properties.

Causes Leading to Formation of Shot Cokes and Measures for Its Prevention in Super Viscous Oil Delayed Coking Unit

Shot cokes are frequently formed in the delayed coking unit treating the super viscous oil at PetroChina＇s Liaohe Petrochemical Company. Considerable work has been carded out to avoid the formation of shot cokes. The test results obtained have shown that the property of super viscous oil has played a key role in the formation of shot cokes. After adjusting and optimizing the process indices the operating regime of the delayed coking unit at a throughput of 118t/h of the super viscous oil is specified as follows： a reaction temperature of 498-502℃, a reaction pressure of 0.17-0.25 MPa, a recycle ratio of 0.5-0.6 and a fractionation tower bottom temperature of 355-365 ℃. In the meantime, the delayed coking process has adopted measures to enhance pre-fractionation of the feedstock to rationally remove light fractions and maintain a steady gas velocity in order to avoid the formation of shot cokes.

Preoperative predictors of portal vein thrombosis after splenectomy with periesophagogastric devascularization

AIM:To evaluate the predictive value of preoperative predictors for portal vein thrombosis(PVT)after splenectomy with periesophagogastric devascularization.METHODS:In this prospective study,69 continuous patients with portal hypertension caused by hepatitis B cirrhosis underwent splenectomy with periesophagogastric devascularization in West China Hospital of Sichuan University from January 2007 to August 2010.The portal vein flow velocity and the diameter of portal vein were measured by Doppler sonography.The hepatic congestion index and the ratio of velocity and diameter were calculated before operation.The prothrombin time(PT)and platelet(PLT)levels were measured before and after operation.The patients'spleens were weighed postoperatively.RESULTS:The diameter of portal vein was negatively correlated with the portal vein flow velocity(P<0.05).Thirty-three cases(47.83%)suffered from postoperative PVT.There was no statistically significant difference in the Child-Pugh score,the spleen weights,the PT,or PLT levels between patients with PVT and without PVT.Receiver operating characteristic curves showed four variables(portal vein flow velocity,the ratio of velocity and diameter,hepatic congestion index and diameter of portal vein)could be used as preoperative predictors of postoperative portal vein thrombosis.The respective values of the area under the curve were 0.865,0.893,0.884 and 0.742,and the respective cut-off values(24.45 cm/s,19.4333/s,0.1138 cm/s-1 and 13.5 mm) were of diagnostically efficient,generating sensitivity values of 87.9%,93.9%,87.9%and 81.8%,respectively,specificities of 75%,77.8%,86.1%and 63.9%,respectively.CONCLUSION:The ratio of velocity and diameter was the most accurate preoperative predictor of portal vein thrombosis after splenectomy with periesophagogastric devascularization in hepatitis B cirrhosis-related portal hypertension.

Forming response of AA5052-H32 sheet deformed using a shock tube

The effect of bending pre-strain and pressure on the forming behavior of AA5052-H32 sheets has been studied using a shock tube. Various forming parameters like dome height, effective strain and stress distribution, hardness, and grain size evolution have been measured. Circular grids are printed on the sheets and Hill’s 1948 yield criterion is used to calculate the effective strain distribution. The effective stress distribution is calculated by using the Hollomon’s power law. The strain evolution during the forming process is monitored by mounting a strain rosette at the mid location of the sheet. The strain-time graph confirms the sharp rise in the peak strain and it increases significantly at higher pressure. The variation in the forming parameters asserts that the material stretches uniformly without strain localization. The optical microstructures also depict that the equiaxed grains are stretched and elongated after the shock deformation. This analysis confirms that the forming behavior of the material is dependent both on the degree of pre-strain and the change in pressure.

Effect of prestressed ultrasonic peen forming parameters on bending curvature and spherical deformation of plate

The elastic prestressed ultrasonic peen forming(UPF)was adopted in order to solve problems of insufficient bending deformation and large spherical deformation of plate during free UPF.The theoretical analysis of prestressed UPF and the influence of elastic prebending moment on deformation were analyzed.Spherical deformation coefficient was defined to quantificationally describe the spherical deformation.Experiments were conducted to compare the differences between free UPF and prestressed UPF processes and the effects of processing parameters on bending curvature and spherical deformation coefficient were studied.The results show that peening trajectory in chordwise direction is beneficial to enlarging spanwise bending deformation and decreasing spherical deformation coefficient.Large prebending curvature is helpful to increase spanwise bending deformation and decrease chordwise deformation,thereby obviously decreasing spherical deformation coefficient.Large spanwise deformation can be obtained under large firing pin velocity,small plate thickness and small offset distance.Large firing pin velocity plays a positive role in decreasing spherical deformation,while plate thickness and offset distance have little effect on it.Above all,prebending curvature and peening trajectory are the most important factors during prestressed UPF process.This study provides guidance for parameters optimization of prestressed UPF for wing plate with large thickness.

Chinese Orthopedic Surgeons' Practice Regarding Postoperative Thromboembolic Prophylaxis after Major Orthopedic Surgery

Objective To assess Chinese surgeon practice of thromboprophylaxis following major orthopedic surgery. Methods A questionnaire survey was conducted amongst Chinese orthopedic surgeons. A total of 293 surgeons were surveyed concerning five key aspects of thromboembolic prophylaxis after major orthopedic surgery at the proseminar of Chinese guidelines for prevention of venous thromboembolism (VTE) after major orthopedic surgery in January of 2009. Results Totally, 208 surgeons (71.0%) responded, successfully completing the questionnaire. Of them, 57.6% respondents selected combined basic, mechanical, and pharmacologic methods for thromboprophylaxis; 51.0% respondents prefer starting prophylaxis 12-24 hours after surgery; 60.3% surgeons would use chemoprophylaxis for 7-10 days; 47.6% respondents prefer VTE prevention based on patients' special conditions and needs upon discharge. "Safety" was the most repeated and emphasized factor during VTE prophylaxis. Conclusions Multimodal thromboprophylaxis is frequently used after major orthopedic surgery. Half surgeons prefer to start chemoprophylaxis 12-24 hours after surgery. Thromboprophylaxis regimen varies for discharged patients.

新课标理念下音乐欣赏该关注什么

音乐是时间的艺术,那些瞬间即逝的音响,形成单音或多音的组合,展现给听觉。听赏与评述是重要的音乐学习任务,是培育学生审美感知和文化理解素养的重要途径。艺术新课标颁布后,音乐欣赏教学被提升到了全新高度。对于一线音乐教师而言,欣赏课似乎比唱歌教学更难。而笔者对欣赏课情有独钟,结合自己的教学感受,总结出了欣赏教学的几点感悟。

Warpage prediction of the injection-molded strip-like plastic parts

For most strip-like plastic injection molded parts, whose cross section size is much smaller than their length, the traditional Hele-Shaw model and three-dimensional model do not work well in the prediction of the warpage be- cause of their special shape. A new solution was suggested in this work. The strip-like plastic part was regarded as a little-curved beam macrnscopically, and was divided into a few one-dimensional elements. On the section of each elemental node location, two-dimensional thermal finite element analysis was made to obtain the non- uniform thermal stress caused by the time difference of the solidification of the plastic melt in the mold. The stress relaxation, or equivalently, strain creep was dealt with by using a special computing model. On the bases of in-mold elastic stress, the final bending moment to the beam was obtained and the warpage was predict- ed in good a^reement with practical cases.

Clinical Observation on Shu Xin Yi Mai Capsules for Prevention of Recurrent Stricture after Coronary Artery Introducing Treatment

Shu Xin Yi Mai Capsules (舒心益脉胶囊) combined with western medicine was used in the routine treatment of 22 cases who successfully received coronary artery introducing therapy (Chinese-western medicine group),and the results was compared with the 26 cases treated routinely with simple western medicine in the control group (western medicine group).It was found that both the recurrence rate of angina pectoris and the incidence rate of recurrent stricture in the Chinese-western medicine group were significantly lower than that in the control group (both P<0.05).There was no significant difference between the two groups in expression of platelet activating molecules CD62P (a-granular membrane protein),CD63 (lysosome intact membrane protein) and CD41 (glucoprotein IIb) before the treatment,but with a significant difference after the treatment (P<0.05).