Prediction of Secondary Structure and B Cell Epitope of GH Protein from Acipenser sinensis

[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.

Relationship between co-stimulatory molecule B7-H3 expression and gastric carcinoma histology and prognosis

AIM:To investigate the expression of co-stimulatorymolecule B7-H3 in gastric carcinoma and adenomatissue as well as normal gastric tissue and to explore therelationship between B7-H3 expression and pathologicalfeatures and prognosis of gastric carcinoma.METHODS:B7-H3 expression was detected in 102samples of human gastric carcinoma and 10 samples ofgastric adenoma and 10 samples of normal gastric tissueby immunohistochemical assay.Correlation betweenthe expression of B7-H3 and the patients'age,sex,gastric carcinoma locus,tumor size,tissue type,tumorinfiltration depth,differentiation degree,lymph nodemetastasis,and survival time was analyzed.RESULTS:B7-H3 was expressed in all gastric adenomasamples and in 58.8% samples of gastric carcinoma.B7-H3 expression in gastric carcinoma samples wasnot related with the patients'age,sex,lymph nodemetastasis,and tumor size(P>0.05),but with thesurvival time,infiltration depth of tumor and tissue type.CONCLUSION:Detection of B7-H3 expression in gastriccarcinoma tissue is beneficial to the judgment of theprognosis of gastric carcinoma patients and the choice oftreatment.

On Self and Other

In the philosophy of mind, the essential qualities of self and selfhood have been discussed widely. According to Zahavi （2015, 149）, ＂the notion of an experiential self is the precondition of any plausible account of intersubjectivity.＂ In Zahavi （2015）, he elaborates two influential views on selfhood and offers a phenomenological perspective from which he argues that selfhood is both a built-in feature of experience and socially constructed. In the following paper, we would further investigate the essential qualities of self in the line with Zahavi （2015） and provide a complementary quality of self from a phenomenological view, namely, the predictability of the expressive phenomena of subjects. We would also explore the intersubjective relationship between others and self and discuss the sharing phenomenon of empathy.

Advances of Bioinformatics Tools Applied in Virus Epitopes Prediction

In recent years,the in silico epitopes prediction tools have facilitated the progress of vaccines development significantly and many have been applied to predict epitopes in viruses successfully. Herein,a general overview of different tools currently available,including T cell and B cell epitopes prediction tools,is presented. And the principles of different prediction algorithms are reviewed briefly. Finally,several examples are present to illustrate the application of the prediction tools.

The Key to Second Language Writing Performance： The Relationship Between Lexical Competence and Writing

Vocabulary knowledge is an important component of the writing skill and it has many dimensions, such as size, depth, and productive, in interaction with writing skill. To evaluate this relation and determine which dimension is the most effective for second language writing quality, the present study was conducted. Turkish EFL （English as a Foreign Language） learners＇ lexical competence and writing abilities were examined through their vocabulary profiles and academic essays. The results of each vocabulary measure indicated that the participants had a limited vocabulary size, containing words mostly from 2,000 to 3,000 frequency bands and thus, the productive vocabulary knowledge of the participants mostly consisted of lk ＋ 2k words and the use of academic words in their essays was very low. The results of the study revealed that the lexical competence covering the main components of vocabulary knowledge was a good predictor of the students＇ quality of writing performance.

ASSIMILATION OF OBSERVED SURFACE WIND WITH GRAPES

With the advances of numerical weather simulation and reduced data assimilation updating cycle, surface observation data assimilation becomes more and more important in data assimilation systems It is widely accepted that a better data assimilation system should contain the restriction of thermod^amic processes in the surface layer. Therefore, in this paper, a new surface wind observation operator is utilized in Global and Regional Assimilation PrEdiction System_3D-Variance （GRAPES_3D-Var）, with the restriction of thermodynamic process in the planetary boundary layer （PBL）. In order to research the ability of this new surface wind observation operator in assimilation and forecasting, a series of experiments are operated by using the GRAPES model. The main results indicate that this new method of surface wind observation operator has positive impact on the forecast with the GRAPES model.

Influence of surface etching pretreatment on PEO process of eutectic Al–Si alloy

To solve the problems generally encountered during the plasma electrolytic oxidation(PEO) of Al alloys with high Si content, a pretreatment of chemical etching was applied before the process. The influence of such pretreatment was studied by SEM, EDS and XRD. The pretreatment presents a significant effect on positive voltage at the beginning stage of PEO, leading to higher voltage over the whole process. The difference between the positive voltages of non-etched and etched specimens decreases gradually with the increase of processing time. The pretreatment exhibits much less influence on the negative voltage. For the sample with surface pretreatment, the average growth rate of PEO coating is increased from 0.50 to 0.84 μm·min-1and the energy consumption is decreased from 6.30 to 4.36 k W·h·μm-1·m-2. At the same time, both mullite and amorphous Si O2 contents are decreased in the coating.

“喻象”的释义与考辨——一段概念史

'喻象'本意为'立体造型',其语义流变肇始于公元前1世纪罗马教育的希腊化。在其发轫阶段,有三位作家发挥了至关重要的作用——瓦罗、卢克莱修和西塞罗。自公元4世纪起,'喻象'除了形象建构的意义之外,还获得了对基督宗教及其圣经解释极为重要的'预表功能','喻象'的用法以及与之相关的阐述方法,在几乎所有拉丁教会作家中得到全面发展。从'形象'到'喻象'到'兆象',一段概念史的考察表明,一个词如何通过语义发展,进入历史环境,并催生历经几个世纪而不衰的结构。促使圣保罗到异教徒中传道的历史环境,发展了兆象阐释,为它在古代晚期乃至中世纪发挥影响铺平了道路。

The active-to-passive oxidation transition mechanism and engineering prediction method of C/SiC composites

This paper studied the active-to-passive oxidative mechanism of C/SiC composite under high temperature and oxidative conditions. An analytic model and computational method were established based on the process of gas diffusion in boundary layer and the equilibrium relations in surface chemical reactions. Simultaneously, an engineering equation to predict the oxygen partial pressure of active-to-passive transition was derived under the specific temperature zone. The results indicated that the active-to-passive oxidation transition of C/SiC is closely related to the composition of the material. At certain temperature and oxygen partial pressure conditions, the composite with high carbon content is prone to cause active oxidation which is negative to the oxidation resistance of the material.

Cancers chemoprevention by dietary phytochemicals and traditional Chinese medicines: epigenetics and cellular signaling pathways

The incidence and mortality of cancer have been increasing alarmingly in China and worldwide.Unlike other chronic diseases such as metabolic,cardiovascular,and neuropathies,most cancers,especially metastatic ones,are still essentially uncontrollable in spite of significant progresses such as early diagnosis and immunotherapies.