天仙果预防关节炎活性部位筛选

采用溶剂法提取分离天仙果不同极性部位,以Freund's完全佐剂型关节炎大鼠模型对天仙果提取分离的各部位进行预防关节炎的药效学筛选。实验表明天仙果醇提、醇提水沉部位具有明显的预防关节炎作用,其中以醇提水沉组效果最佳,即中等极性部位具有预防关节炎作用。

预防关节炎不嫌早

冬季,在被寒风侵扰的同时,大家的关节健康也遭遇了考验,与朋友聚会、相约外出,都可能被突如其来的膝盖疼痛搅了局,而这样的膝盖疼痛很多会被诊断为关节炎。近年来门诊接诊的关节炎患者,除了老年人之外,年轻人也越来越多。在我国,关节炎患者约有1.2亿人,几乎每10人中就有1人患有骨性关节炎,且发病群体年轻化的趋势愈加明显。

丹参加玻璃酸钠预防性关节内注射对兔膝关节软骨退行性变的影响

目的通过观察丹参加玻璃酸钠预防性关节内注射对兔膝关节软骨退行性变的影响,探讨其治疗作用机理。方法将80只日本大耳白兔随机分为A组、B组、C组、D组,每组20只。通过切断膝关节前交叉韧带和内侧副韧带造成膝OA模型,每组每只动物分别注入0.3 ml的生理盐水(A组)、玻璃酸钠注射液(B组)、丹参注射液(C组)、丹参加玻璃酸钠混合液(D组),手术当天给药,每周1次。于给药4、8周后分批处死实验兔,每批每组各10只。行关节腔大体观察、光镜下组织学观察、凋亡软骨细胞检测。结果各组术后4、8周组间比较,软骨退变计分、软骨凋亡细胞计数,有无显著性意义;D组与B、C组之间差异无显著性,但D组的均值小于B、C组(P>0.05);各组术后4、8周组内比较,B、C、D组软骨退变计分、软骨凋亡细胞计数相比较,差异无显著性(P>0.05);A组软骨退变计分、软骨凋亡细胞计数相比较,有显著性差异(P<0.05)。结论在OA早期,预防性关节腔注射丹参和HA,能明显延缓关节软骨的蜕变,为早期防治OA提供实验基础。

丹参加玻璃酸钠关节内注射对家兔实验性膝骨性关节炎软骨退变的影响

为探讨预防性关节内注射丹参加玻璃酸钠时骨关节炎软骨退变和细胞凋亡的影响，将成年健康日本大耳白兔37只随机分为4组。A组9只，B组9只。C组10只，D组9只。手术切开右后肢膝关节，搜刮髌上囊前壁，推开髌上囊后壁，切除髌下脂肪垫，缝合关节囊厦皮肤后关节穿刺。每组每只动物分别注入0．3ml的生理盐水（A组）、玻璃酸钠注射液（B组）、丹参注射液（C组）、丹参加玻璃酸钠混合液（各0、15ml，D组），管形石膏固定患肢于屈膝70—90°位；4周后处死动物，行关节腔大体观察，股骨髁下面关节软骨组织学观察和细胞凋亡检测。结果显示，关节软骨退变计分B、C、D组均小于A组（（P〈0.01）。D组的均值小于B、C组（P〉0．05）；关节软骨凋亡细胞计数B、C、D组均小于A组（P〈0．01），D组的均值小于B、C组（P〉0．05）。表明丹参能减轻软骨退变程度、减少软骨凋亡细胞数目。有效预防骨关节炎；丹参加玻璃酸钠与二药单独使用比较，有减轻软骨退变程度、减少软骨凋亡细胞数目的趋势，可以提高预防骨关节炎。

关节腔内注射洛伐他汀预防骨关节炎软骨退变

目的研究洛伐他汀关节腔内注射对骨关节炎（OA）模型关节软骨退变及对基质金属蛋白酶（matrix metalloproteinase-1，3）mRNA表达的影响。方法30只6个月龄新西兰大白兔行右膝关节前交叉韧带切断术。手术后将动物随机分为实验组和对照组，实验组术后立即给予关节腔内注射0．5mg／ml洛伐他汀0．2ml／kg，每周1次，连续6周；对照组则关节腔内注射等容量的生理盐水。术后6周处死动物。在解剖镜下观察股骨内髁关节软骨的大体形态学改变并评分。用反转录一聚合酶链式反应检测软骨及滑膜中MMP-1，3mRNA的表达。结果解剖镜下实验组软骨退变程度较对照组明显减轻；实验组关节液中IL-1水平较对照组明显降低；实验组滑膜中MMP-1，3mRNA的表达水平显著低于对照组（P〈0．05），而软骨中的MMP-1，3mRNA的表达较对照组差异无统计学意义（P〉0．05）。结论在OA早期关节腔内注射L洛伐他汀能够明显降低MMP-1，3mRNA的表达，减轻骨关节炎软骨的退变。

Preventive Administration of Juanbi Capsules (蠲痹胶囊) for Knee Osteoarthritis: Effects on Serum MMP-2 and MMP-9 levels and Cartilage Repair

Objective: To explore the mechanism of action of Juanbi Capsules (蠲痹胶囊), a Chinese medicine for invigorating the kidney and replenishing qi, in preventing osteoarthritis of the knee in rabbits. Methods: Seventy-two 4-month-old, Japanese long-eared white rabbits were randomly divided into 6 groups: control (group A), model (group B), Chinese drug; high-dose (group C), Chinese drug; mid-dose (group D), Chinese drug; low-dose (group E), and drug control (group F). With the exception of the rabbits in group A, each rabbit was subjected to plaster cast fixation for 6 weeks to induce osteoarthritis. In addition, rabbits were administrated with an intragastric injection of the Chinese drug (groups C, D and E) or an aminoglucose hydrochloride capsule (group F) for 4 weeks. Blood was drawn from the central ear artery for serum MMP-2 and MMP-9 concentrations, and the knee joint cartilage was harvested for gross observation and light microscopy. Results: There were significant differences in serum MMP-2 and MMP-9 concentrations between group B and groups C, D and E (P<0.05), with no significant differences between groups D and F. Histological results showed various changes in tissue staining with treatment, with osteophyte and bone cyst formation, and superficial erosion in the articular surface of the cartilage; in some cases, the defect reached the mid-layer of the cartilage, and these changes were lower than those in the model group. Conclusion: Juanbi Capsules assist in preventing osteoarthritis in the rabbit, possibly by decreasing serum MMP-2 and MMP-9 levels.

Effects of Preventive Administration of Juanbi Capsules on TNF-α、 IL-1 And IL-6 Contents of Joint Fluid in the Rabbit with Knee Osteoarthritis

Objective:To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing qi for preventing knee osteoarthritis of the rabbit. Methods: Totally 72 healthy Japan long-ear white rabbits, aged 4 months, were randomly divided into 6 groups, blank group (A), model group (B), high dose Chinese herb group (C), middle dose Chinese herb group (D), small dose Chinese herb group (E), aminoglucose hydrochloride capsule control group (F), 12 rabbits in each group. All the rabbits in the groups, except the group A, were fixed with plaster cast for six weeks to establish rabbit knee osteoarthritis. At the same time of modeling, the different doses of Juanbi Capsules and aminoglucose hydrochloride capsule were administrated intragastrically in the group C, D, E, F, respectively, for 4 weeks, for preventive treatment. In the group B, the rabbit was administrated intragastrically with equal volume of normal saline to the medication groups, twice each day, in the morning and the evening, and in the group A, nothing was administrated. After modeling for 6 weeks, the joint fluid was taken and TNF-α, IL-1 and IL-6 contents were detected with ELISA method, and the articular cartilage was taken for macroscopic and microscopic examinations. Results: In all the preventive treatment groups, the articular cartilage color changed to varying degrees with formation of osteophyte and bone cyst, superficial erosion on the chondral articular surface, and the cartilage defect reached to the mid layer in a part of specimens with cartilage exfoliation, but which in the extent were significantly lower than those in the model group. There were significant differences between the group A and B in TNF-α , IL-1 and IL-6 contents in the joint fluid (P<0.05), indicating that the modeling is successful; and there were significant differences as group B compared with the group C,D, E, F, showing that TNF-α , IL-1 and IL-6 contents are decreased in all the medication groups; and significant differences between group C, D, E suggests that the increase of Chinese herb doses strengthened the effect of reducing TNF-α , IL-1 and IL-6 contents in joint fluid. Conclusion: The Juanbi Capsule prevents osteoarthritis possibly through decreasing serum TNF-α, IL-1 and IL-6 contents.