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不同频率摩腹对脾虚型功能性消化不良家兔胃肠动力的调控规律及其与CaM-MLCK信号通路相关性研究
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作者 张强 王继红 +3 位作者 黄志凯 蔡伟蓝 任雪晗 许嘉英 《环球中医药》 CAS 2023年第10期1981-1987,共7页
目的观察不同频率摩腹对脾虚型功能性消化不良家兔胃肠动力的调控规律,以钙调蛋白—肌球蛋白轻链激酶(caImodulin-myosin light chain kinase,CaM-MLCK)信号通路为切入点探讨摩腹调控胃肠动力的作用机制。方法随机将60只健康成年新西兰... 目的观察不同频率摩腹对脾虚型功能性消化不良家兔胃肠动力的调控规律,以钙调蛋白—肌球蛋白轻链激酶(caImodulin-myosin light chain kinase,CaM-MLCK)信号通路为切入点探讨摩腹调控胃肠动力的作用机制。方法随机将60只健康成年新西兰家兔均分为空白组、模型组、低频摩腹治疗组、高频摩腹治疗组、低频摩腹+抑制剂组及高频摩腹+抑制剂组。除空白组外,其余各组家兔均造脾虚型功能性消化不良模型,空白组及模型组均不施加干预。治疗组采用人工智能机械臂以相应频率摩腹作用于各组家兔中脘、天枢穴(双),保持摩腹压力与方向一致,低频摩腹治疗组及低频摩腹+抑制剂组摩腹频率为101~150次/分钟,高频摩腹治疗组及高频摩腹+抑制剂组频率为201~250次/分钟,每只家兔每穴推5分钟,共计15分钟,每日1次。低频摩腹+抑制剂组及高频摩腹+抑制剂组均在每日摩腹治疗给家兔腹腔注射肌球蛋白轻链激酶抑制剂(ML 9 hydrochloride,ML-9),连续干预10天。干预结束后采用炭末灌胃法测定各组家兔小肠推进率,并测定各组家兔胃肠组织一氧化氮(nitric oxide,NO)、P物质(Substance P,SP)、腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)、三磷酸肌醇(Inositol Triphosphate,IP3)及环磷酸腺苷(cyclic adenosine monophosphate,cAMP)的含量。结果与空白组相比,模型组小肠推进率显著降低(P<0.01),胃NO含量显著升高(P<0.01),胃SP含量显著下降(P<0.01),胃、肠IP3无统计学差异(P>0.05),胃、肠ATP含量下降(P<0.05),胃、肠cAMP含量升高(P<0.05)。干预结束后,与模型组相比,低频摩腹治疗组、低频摩腹+抑制剂组及高频摩腹+抑制剂组小肠推进率均显著提高(P<0.01),高频摩腹治疗组无统计学差异(P>0.05);与模型组相比,低频摩腹治疗组胃NO含量显著下降(P<0.01),胃SP含量显著升高(P<0.01),肠IP3下降(P<0.05),胃、肠ATP含量升高(P<0.05),胃、肠cAMP含量下降(P<0.05);高频率摩腹治疗组胃SP含量升高(P<0.01),肠IP3含量下降(P<0.05),肠ATP含量升高(P<0.05),其余指标无统计学差异(P>0.05)。与低频摩腹治疗组比较,低频摩腹+抑制剂组胃NO含量升高(P<0.05),胃SP含量无统计学差异(P>0.05),肠IP3显著下降(P<0.01),胃、肠ATP含量显著下降(P<0.01),胃、肠cAMP含量升高(P>0.05);与高频摩腹治疗组比较,高频摩腹+抑制剂组胃NO含量升高(P<0.05),胃SP含量无统计学差异(P>0.05),肠IP3显著下降(P<0.01),胃、肠ATP含量下降(P<0.05),胃、肠cAMP含量升高(P<0.05)。结论摩腹对胃肠动力的影响存在频率—效应规律,摩腹在101~150次/分钟频率段时可显著促进胃肠动力,当频率超过200次/分时,摩腹对胃肠动力无改善作用,甚至有损害趋势。其作用机制可能是:(1)通过降低胃肠组织中NO及cAMP的含量,抑制cAMP/PKA途径及cGMP/PKG途径,减少对Ca 2+-CaM的竞争性消耗,从而促进上游CaM-MLCK信号通路中Ca 2+、Ca 2+-CaM与MLCK的结合。(2)当CaM-MLCK信号通路被阻断后,一方面,摩腹可通过提高家兔胃SP含量,发挥其促进胃肠动力的直接效应;另一方面,IP3的显著下降提示,阻断CaM-MLCK信号通路,可能增加IP3的消耗促使SOCE发生,从而改变胞内Ca 2+水平,激发Ca 2+介导的其它信号通路来调节胃肠动力。 展开更多
关键词 频率—效应 摩腹 功能性消化不良 胃肠动力 CaM-MLCK信号通路
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Effects of Collocational Congruency,Frequency and L2 Proficiency on the Processing of English Adjective-Noun Collocations
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作者 Lianrui YANG Qun ZHANG +1 位作者 Ni LI Ying CHEN 《Chinese Journal of Applied Linguistics》 2024年第3期393-413,524,共22页
The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online accep... The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online acceptability judgment task.The subject pool of this research included 60 English majors studying at a university in China;30 were selected as a higher-proficiency group and 30 as a lower-proficiency group according to their Vocabulary Levels Test(Schmitt et al.,2001)scores and their self-reported proficiency in English.The experimental materials were programmed to E-prime 2.0 and included six types of collocations:(1)15 high-frequency congruent collocations,(2)15 low-frequency congruent collocations,(3)15 high-frequency incongruent collocations,(4)15 low-frequency incongruent collocations,(5)15 Chinese-only items,and(6)75 unrelated items for baseline data.The collected response times(RTs)and accuracy rates data were statistically analyzed by the use of an ANOVA test and pairwise comparisons through SPSS 16.0 software.The results revealed that:(1)the adjective-noun collocational processing of Chinese English learners is influenced by collocational frequency,congruency and L2 proficiency;(2)the processing time is affected by the interaction of congruency and frequency;and(3)the interactive effect of L2 proficiency in conjunction with congruency and frequency also influences the processing quality. 展开更多
关键词 adjective-noun collocations congruency effects frequency effects L2 proficiency
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