颗石藻颗石粒形态的原子力显微观测方法:以赫氏艾密里藻为例

颗石藻(coccolithophore)作为一种模式生物,在重建古海洋气候和环境以及预测未来全球气候变化中起着很重要的作用,赫氏艾密里藻(Emiliania huxleyi)是颗石藻最为典型的代表种。钙质颗石粒(coccolith)是颗石藻形态分类的主要依据,有着非常精细和复杂的结构,在样品收集过程中很容易遭到破坏,这是颗石藻鉴定中经常遇到的一个技术问题。国际上还没有统一的颗石藻定量采样和样品分析方法。本文采用原子力显微方法(atomic force microscopy,AFM)对赫氏艾密里藻的颗石粒形态进行了超显微观察研究,获取不同扫描范围的高度图(height image)和形貌图(deflection image)以观测其形态结构,并建立了针对颗石藻的原子力显微样品制备方法。通过离心与膜过滤两种方法收集赫氏艾密里藻,比较后得出了一种简单、快速的适合于观测颗石藻在大气环境成像的样品处理、制备和图像采集方法:3,000–4,000 rpm,20℃离心5 min,收集颗石藻,去除有机杂质后取白色沉淀,将沉淀物悬浮于0.05 M NH_4HCO_3溶液中,悬浮液滴加于盖玻片表面,20℃晾干后于样品台在AFM接触模式(contact mode)下原子级扫描,扫描范围50μm,频率1 Hz,可以得到优质的颗石粒形态图像,有助于颗石藻的分类鉴别。该方法可用于室内不同环境梯度或参数下的颗石粒形态结构及颗石藻藻华的检测与研究。

3D characterization and analysis of pore structure of packed ore particle beds based on computed tomography images

Methods and procedures of three-dimensional （3D） characterization of the pore structure features in the packed ore particle bed are focused. X-ray computed tomography was applied to deriving the cross-sectional images of specimens with single particle size of 1-2, 2-3, 3-4, 4-5, 5-6, 6-7, 7-8, 8-9, 9-10 ram. Based on the in-house developed 3D image analysis programs using Matlab, the volume porosity, pore size distribution and degree of connectivity were calculated and analyzed in detail. The results indicate that the volume porosity, the mean diameter of pores and the effective pore size （d50） increase with the increasing of particle size. Lognormal distribution or Gauss distribution is mostly suitable to model the pore size distribution. The degree of connectivity investigated on the basis of cluster-labeling algorithm also increases with increasing the particle size approximately.

Electro-flotation and collision-attachment mechanism of fine cassiterite

In order to discuss the particle-bubble interaction during the electro-flotation of cassiterite,the recovery of cassiterite with different particle sizes was investigated,and the collision mechanism between the cassiterite particles and H2 bubbles was explored.The flotation tests were carried out in a single bubble flotation cell.The results show that cassiterite particles 10 μm,10-20 μm,20？38 μm and 38-74 μm match with bubbles with size of 50-150 μm,about 250 μm,74 μm and 74 μm,respectively,and a better recovery can be obtained.It is demonstrated that the recovery of cassiterite is influenced by the size of cassiterite particles and bubbles.Furthermore,the probabilities of collision,adhesion,detachment and collection were calculated using the collision,attachment and collection models.Theoretical calculation results show that the collision probability decreases sharply with decreasing particle size and increasing bubble size（below 150 μm）.The attachment probability would increase from the effective collision,leading to the increase of recovery.

Collision and attachment behavior between fine cassiterite particles and H_2 bubbles

Particle-bubble interaction during electro-flotation of cassiterite was investigated by determining the recovery of cassiterite and the collision mechanism of cassiterite particle and H2 bubble. Flotation tests at different conditions were conducted in a single bubble flotation cell. The recovery of cassiterite was found to be affected by cassiterite particle and bubble size. A matching range, in which the best recovery can be obtained, was found between particle and bubble size. Collision, attachment, and detachment of the particle-bubble were observed and captured by a high-speed camera. Particle-bubble collision and attachment were analyzed with the use of particle-bubble interaction theory to obtain the experimental results. An attachment model was introduced and verified through the photos captured by the high-speed camera. A bridge role was observed between the bubbles and particles. Particle-bubble interaction was found to be affected by bubble size and particle size, which significantly influenced not only the collision and attachment behavior of the particles and bubbles but also the flotation recovery of fine cassiterite particles.

High Performance Ultraviolet Photodetector Fabricated with ZnO Nanoparticles-graphene Hybrid Structures

Ultraviolet （UV） photodetector constructed by ZnO material has attracted intense research and commercial interest. However, its photoresistivity and photoresonse are still unsatisfied. Herein, we report a novel method to assemble ZnO nanoparticles （NPs） onto the reduced graphite oxide （RGO） sheet by simple hydrothermal process without any surfactant. It is found that the high-quality crystallized ZnO NPs with the average diameter of 5 nm are well dispersed on the RGO surface, and the density of ZnO NPs can be readily controlled by the concentration of the precursor. The photodetector fabricated with this ZnO NPs- RGO hybrid structure demonstrates an excellent photoresponse for the UV irradiation. The results make this hybrid especially suitable as a novel material for the design and fabrication of high performance UV photodector.

Damage mechanism of soil-rock mixture after freeze-thaw cycles

As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the damage mechanism is not clear.Based on the damage factor,the damage research of properties of soil-rock mixture after different times of freeze-thaw cycles is investigated.Firstly,the size-distributed subgrade gravelly soil samples are prepared and undergo different times of freeze-thaw cycles periodically(0,3,6,10),and indoor large-scale triaxial tests are completed.Secondly,the degradation degree of elastic modulus is considered as a damage factor,and applied to macro damage analysis of soil-rock mixture.Finally,the mesoscopic simulation of the experiments is achieved by PFC3D,and the influence on strength between soil-rock particles caused by freeze-thaw cycles is analyzed.The results show that freeze-thaw cycles cause internal damage of samples by weakening the strength between mesoscopic soil-rock particles,and ultimately affect the macro properties.After freeze-thaw cycles,on the macro-scale,elastic modulus and shear strength of soil-rock mixture both decrease,and the decreasing degree is related to the times of cycles with the mathmatical quadratic form;on the meso-scale,freeze-thaw cycles mainly cause the degradation of the strength between soil-rock particles whose properties are different significantly.

Preparation of low-temperature sintered high conductivity inks based on nanosilver self-assembled on surface of graphene

Finer nanoplates of silver are prepared by self-assembly on the surface of graphene,and the low-temperature sintered high conductivity ink containing the silver nanoplates is prepared.Most importantly,graphene is added to the solution before the chemical reduction reaction occurs.Firstly,it is found that silver nanoplates have self-assembly phenomenon on the surface of graphene.Secondly,the Ag nano hexagonal platelets(AgNHPs)with small particle sizes(10 nm),narrow distribution and good dispersion are prepared.Especially,smaller sizes(10 nm)and narrower particle size distribution of AgNHPs particles can be easily controlled by using this process.Finally,the conductivity of the ink is excellent.For example,when the printed patterns were sintering at 150℃,the resistivity of the ink(GE:0.15 g/L)reached the minimum value of 2.2×10^-6 cm.And the resistivity value was 3.7×10^-6Ωcm,when it was sintered at 100℃ for 30 min.The conductive ink prepared can be used for the field of printing electronics as ink-jet printing ink.

Thermo-mechanical coupled particle model for rock

A thermo-mechanical coupled particle model for simulation of thermally-induced rock damage based on the particle simulation method was proposed.The simulation results of three verification examples,for which the analytical solutions are available,demonstrate the correctness and usefulness of the thermo-mechanical coupled particle model.This model is applied to simulating an application example with two cases：one is temperature-independent elastic modulus and strength,while the other is temperature-dependent elastic modulus and strength.The related simulation results demonstrate that microscopic crack initiation and propagation process with consideration of temperature-independent and temperature-dependent elastic modulus and strength are different and therefore,the corresponding macroscopic failure patterns of rock are also different.On the contrary,considering the temperature-dependent elastic modulus and strength has no or little effect on the heating conduction behavior.Numerical results,which are obtained by using the proposed model with temperature-dependent elastic modulus and strength,agree well with the experimental results.This also reveals that the rock subjected to heating experiences much more cracking than the rock subjected to cooling.

Comparison between FLO-2D and Debris-2D on the Application of Assessment of Granular Debris Flow Hazards with Case Study

Numerical simulation has been widely applied to the assessment of debris flow hazards. In East Asia and especially Taiwan, the most widely used numerical programs are FLO-2D and Debris-aD. Although these two programs are applied to the same engineering tasks, they are different in many aspects. These two programs were compared according to their fundamental theories, input and output data, computational algorithms and results. Using both programs, the simulations of a real debris flow with abundant granular material induced by landslides at Xinfa village in southern Taiwan are performed for comparison. The simulation results show that Debris- 2D gives better assessment in hazard area delineating and flow depth predicting. Therefore, Debris-2D is better for simulation of granular debris flows.

Pore structure of ore granular media by computerized tomography image processing

The pore structure images of ore particles located at different heights of leaching column were scanned with X-ray computerized tomography (CT) scanner, the porosity and pore size distribution were calculated and the geometrical shape and connectivity of pores were analyzed based on image process method, and the three dimensional reconstruction of pore structure images was realized. The results show that the porosity of ore particles bed in leaching column is 42.92%, 41.72%, 39.34% at top, middle and bottom zone, respectively. Obviously it has spatial variability and decreases appreciably along the height of the column. The overall average porosity obtained by image processing is 41.33% while the porosity gotten from general measurement method in laboratory is 42.77% showing the results of both methods are consistent well. The pore structure of ore granular media is characterized as a dynamical space network composed of interconnected pore bodies and pore throats. The ratio of throats with equivalent diameter less than 1.91 mm to the total pores is 29.31%, and that of the large pores with equivalent diameter more than 5.73 mm is 2.90%.

Grain Composition and Erosive Equilibrium of Debris Flows

Debris flows consist of grains of various sizes ranging from 10^(-6) m ～1 m. Field observations in the Jiangjia Gully (JJG) and other sites throughout China indicate that the grain size distribution of sediment in debris flows can be characterized by an exponential function fit to the cumulative distribution. The exponent value for the function varies by location and may be useful in distinguishing between debris flows from different valleys. For example, minimum values and ranges of the exponent are associated with the high frequency of debris flows in the JJG. Furthermore, the distribution presents piecewise fractality (i.e. scaling laws hold in various ranges of the grain size) and we propose that the fractal structure determines the matrix and that the fractal dimension plays a crucial role in material exchange between a debris flow and the substrate it flows over. Finally, the empirical data support an exponential relation between grain composition and non-dimensional shear stress for the critical state of the channel. Overall we propose a material-determinism approach to studying debris flows which contrasts with the enviro-determinism that has dominated much recent work in this field.

Variation in Grain Size Distribution in Debris Flow

Grain composition of debris flow varies considerably from fluid to deposit, making it uncertain to estimate flow properties （e.g., density, velocity and discharge） using deposit as done in practice. Tracing the variation of grain composition is thus more important than estimating some certain properties of flow because every debris flow event consists of a series of surges that are distinct in properties and flow regimes. We find that the materials of debris flows, both the fluid and the source soils, satisfy a universal grain size distribution （GSD） in a form of P （D） = CD-zexp（-D/Dc）, where the parameters C, p and De are determined by fitting the function to the grain size frequency. A small At implies a small porosity and possible high excess pore pressure in flow; and a large D~ means a wide range of grain composition and hence a high sediment concentration. Flow density increases as 11 decreases or Dc increases, in a power law form. A debris flow always achieves a state of certain mobility and density that can be well described by the coupling of p and Dc, which imposes a constraint on the fluctuations of flow surges. The GSD also describes the changes in grain composition in that it is always satisfied during the course of debris flow developing. Numerical simulation using the GSD can well illustrate the variation ofμ and Dc from source soils to deposits.

Study on Tribological Properties of the Rolling Fluid Containing Nano-MoS_2 for Cold Rolling of Steel Strip

The nano-MoS2 particles were adopted to substitute for the extreme-pressure and anti-wear additives to the rolling fluid for steel strips.An optimal formulation and technical process for adding nano-MoS2 particles to the rolling fluid were obtained through orthogonal experiments with three factors at three levels.The tests have led to the following conclusions:(a) the nanoparticles should be added to a base oil with high saponification value;(b) the concentration of nanoparticles in rolling fluid should be equal to 0.6%;and (c) when the reaction time needed for surfactants (oleic acid,for example) to modify the nanoparticles was 40 min,the rolling fluid could have the best tribological properties.The lubricity of rolling fluid was verified by using a 4-high cold rolling test mill which showed that the rolling fluid with nano-MoS2 particles had the excellent lubricant performance to improve the surface quality of the steel strip remarkably.The mechanism of nano-MoS2 particles in the rolling process has been investigated by a series of characterization instruments,which have showed that,with its fullerene-like structure,the nano-MoS2 particles have self-lubricating properties and extra-low friction coefficient,and can easily form a protective film with low shear strength on the surface of friction pairs.

The Effect of Coupling Agents on Natural Zeolite-Filled Natural Rubber

Natural zeolite particles of 40, 80 and I00 phr were incorporated into natural rubber （NR） using a two-roll mill. The surface treatment of zeolite particles was expected to improve their cure characteristics and their tensile properties. Two types of silane coupling agents, i.e., bis-[3-（triethoxysilyl）-propyl]-tetrasulfide （Coupling 89） and 3-Octanoylthio-l-propyltriethoxysilane （NXT） at various loadings of 2, 4 and 8 percent by weight （%wt） were selected. The viscosity and cure time of the rubber compound increased, while the scorch time decreased after the treatment of zeolite by both coupling agents. The viscosity of the rubber compound predominantly increased when increasing Coupling 89, however, NXT loadings showed no significant effect on the cure characteristics Moreover, the amount and type of coupling agent influenced the tensile properties of the vulcanizates, as a result of the increase in tensile strength, and tensile modulus with increasing both coupling agents loading. The tensile properties of the vulcanizates increased at low Coupling 89 concentrations （2-4%wt）, but decreased at high Coupling 89 loading （8%wt）. Since NXT has a less bulky structure and lower viscosity compared with Coupling 89, it thus reacts with the silanol groups on the zeolite surface more readily, resulting in the decrease in filler-filler interaction, leading to better tensile properties.

Tribological and corrosion behavior of PEO coatings with graphite nanoparticles on AZ91 and AZ80 magnesium alloys

The effect of the addition of graphite nanoparticles into the electrolyte used to produce plasma electrolytic oxidation（PEO） coatings on AZ91 and AZ80 magnesium alloys was studied. The corrosion and wear resistances of the obtained coatings were investigated. A solution that contained both phosphates and silicates was used as electrolyte. Moreover, two different PEO treatment times were studied. The corrosion resistance was analyzed with potentiodynamic polarization and EIS tests; the wear resistance was investigated with a flat on ring tribometer. The results were related to the morphology, microstructure, elemental composition and thickness evaluated with SEM analysis. The presence of the graphite nanoparticles increased the thickness, produced a densification of the coating and sealed the pores on the surface, thus improving both the corrosion and wear resistance. The increase in the corrosion and wear resistances was more evident for AZ91 than for AZ80 due to the higher aluminum content.

Effect of Tb/Mg doping on composition and physical properties of hydroxyapatite nanoparticles for gene vector application

Transfection efficiency of hydroxyapatite nanoparticles(HAnps)is relative to the particle size,morphology,surface charge,surface modifier and so on.This study prepared HAnps with doped Tb/Mg by hydrothermal synthesis method(HTSM)and investigated the effects of different Tb/Mg contents on the morphology,particle size,surface charge,composition and cellular endocytosis of HAnps.The results showed that Mg-HAnps possessed better dispersion ability than Tb-HAnps.With increasing doping content of Tb/Mg-HAnps,the granularity of Tb-HAnps increased,while that of Mg-HAnps declined.Both particle size and zeta potential of Mg-HAnps were lower than those of Tb-HAnps.7.5%Mg-doping HAnps presented relatively uniform slender rod morphology with average size of30nm,while10%Mg-doping HAnps were prone to agglomeration.Moreover,Mg-HAnps-GFP(green fluorescent protein)endocytosed by MG63cells was dotted in the perinuclear region,while Tb-HAnps were more likely to aggregate.In conclusion,as gene vectors,Mg-HAnps showed enhanced properties compared to Tb-HAnps.

Quantitative determination of PFC3D microscopic parameters

It is important to calibrate micro-parameters for applying partied flow code(PFC)to study mechanical characteristics and failure mechanism of rock materials.Uniform design method is firstly adopted to determine the microscopic parameters of parallel-bonded particle model for three-dimensional discrete element particle flow code(PFC3D).Variation ranges of microscopic of the microscopic parameters are created by analyzing the effects of microscopic parameters on macroscopic parameters(elastic modulus E,Poisson ratio v,uniaxial compressive strengthσc,and ratio of crack initial stress to uniaxial compressive strengthσci/σc)in order to obtain the actual uniform design talbe.The calculation equations of the microscopic and macroscopic parameters of rock materials can be established by the actual uniform design table and the regression analysis and thus the PFC3D microscopic parameters can be quantitatively determined.The PFC3D simulated results of the intact and pre-cracked rock specimens under uniaxial and triaxial compressions(including the macroscopic mechanical parameters,stress−strain curves and failure process)are in good agreement with experimental results,which can prove the validity of the calculation equations of microscopic and macroscopic parameters.

Influence of intermetallic Al-Mn particles on in-situ steam Mg-Al-LDH coating on AZ31 magnesium alloy

The influence of intermetallic Al-Mn particles on the corrosion behavior of in-situ formed Mg-Al layered double hydroxide(Mg-Al-CO32--LDH)steam coating on AZ31 Mg alloy was investigated.The alloy was pretreated with H3PO4,HCl,HNO3or citric acid(CA),followed by hydrothermal treatment,for the fabrication of Mg-Al-LDH coating.The microstructure,composition and corrosion resistance of the coated samples were investigated.The results showed that the surface area fraction of Al-Mn phase exposed on the surface of the alloy was significantly increased after CA pretreatment,which promotes the growth of the Mg-Al-LDH steam coating.Further,the LDH-coated alloy pretreated with CA possessed the most compact surface and the maximum coating thickness among all the coatings.The corrosion current density of the coated alloy was decreased by three orders of magnitude as compared to that of the bare alloy.

Origin of Red Color of the Lower Siwalik Palaeosols:A Micromorphological Approach

Palaeosols associated with fluvial of the Siwalik Group are and lacustrine deposits that occur as thick multiple pedocomplexes. The bright red color of the palaeosol beds has been earlier interpreted as a result of hot and arid palaeoclimate. However, as against this view, our investigations of the bright red palaeosol beds of the Lower Siwaliks suggest that the climate was cool and subhumid, instead of hot and arid during the deposition of these beds. Since cold climate is not very conducive to impart red coloration, further research is needed to explain the cause of these red beds. For this, the micromorphological study of soil thin sections was done which showed the presence of features such as dissolution and recrystallisation of quartz, feldspar and mica, compaction, slickensides, presence of calcite spars, subrounded and cracked nature of quartz grains, microfabric, complex patterns of birefringence fabrics, pigmentary ferric oxides, thick cutans and cementation by calcite. These features indicate that diagenesis took place on a large scale in these sediments. The positive Eh and neutral-alkaline pH of soils also suggest that the conditions were favorable for the formation of diagenetic red beds. During burial diagenesis of sediments, the hydroxides of ferromagnesian minerals got converted into ferric oxide minerals （hematite）. During deep burial diagenesis smectite was converted into illite and the preponderance of illite over smectite with increasing depth of burial also indicates the diagenesis of sediments. Thus, the red color of the Lower Siwalik palaeosols seems to be due mainly to the burial diagenesis of sediments and does not appear to be due to the then prevailing climatic condition.

Influence of surface roughness on flotation kinetics of quartz

Surface roughness of quartz particles was determined by measuring the specific surface area of particles.The wettability characteristics of particles were determined by measuring the flotation rate using a laboratory flotation cell.Experimental results show that the rod mill product has higher roughness than the ball mill product.For the particles with larger surface roughness,the flotation kinetics constant is also higher.Finally,empirical relationships between surface roughness(r) and the flotation kinetics constant(k) of quartz particles as k=A+Br+Cr0.5lnr+D/lnr+E/r and k=A+Br are presented,in which A,B,C,D and E are constants related to experimental conditions and mineralogical properties of mineral.