[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 m...[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 mm × 150 mm,5 μm).The mobile phase was acetonitrile-water(35∶65).The ELSD evaporator tube temperature was 65 ℃.N2 was used as the carrier gas(pressure,30 psi).[Result] When the content of Astragaloside IV ranged from 0.5 to 5.0 μg,the Astragaloside IV content showed a good linear relationship with peak area(r=0.999,n=6).The average recovery was 96.36%,and the RSD was 2.46%.[Conclusion] This method is accurate and reliable,and can be applied in the quality control of Yupingfeng oral solution.展开更多
AIM: To investigate the expression of Popeye domain containing 3 (Popdc3) and its correlation with clinicopathological features and prognosis of gastric cancer.METHODS: The method of immunohistochemistry was used ...AIM: To investigate the expression of Popeye domain containing 3 (Popdc3) and its correlation with clinicopathological features and prognosis of gastric cancer.METHODS: The method of immunohistochemistry was used to investigate the expression of Popdc3 in 306 cases of human gastric cancer and 84 noncancerous gastric tissues. Simultaneously, the relationship between Popdc3 expression and the survival of the patients was retrospectively analyzed.RESULTS: Popdc3 was detected in 72 (85.71%) of 84 human nontumor mucosa. High expression of Popdc3protein was detected in 78 (25.49%) of 306 human gastric cancer cases, and low expression was detected in 228 (74.51%). Low expression of Popdc3 correlated with depth of invasion (P 〈 0.0001), regional lymph nodes (P 〈 0.0001) and distant metastasis (P =0.02), and tumor, nodes, metastasis (TNM) stages (P 〈 0.0001). On multivariate analysis, only the patient's gender, regional lymph node metastasis, distant metastasis, TNM stages, and the expression of Popdc3 were independent prognostic factors in patients with gastric cancer. The Kaplan-Meier plot showed that low Popdc3 expression had a much more significant effect on the survival of those patients with early-stage tumors X^2 = 104.741, P 〈 0.0001), with a 〉 51.9% reduction in the three-year survival compared with high Popdc3 expression. In late stages, the difference was also significant X^2 = 5.930, P = 0.015), with a 32.6% reduction in the three-year survival.CONCLUSION: Reduced expression of Popdc3 may play a significant role in the carcinogenesis and progression of gastric cancer. Popdc3 may be an independent prognostic factor.展开更多
A quantitative risk assessment (QRA) based on leak detection criteria (LDC) for the design of a proposed subsea oil export pipeline is presented in this paper. The objective of this QRA/LDC study was to determine ...A quantitative risk assessment (QRA) based on leak detection criteria (LDC) for the design of a proposed subsea oil export pipeline is presented in this paper. The objective of this QRA/LDC study was to determine if current leak detection methodologies were sufficient, based on QRA results, while excluding the use of statistical leak detection; if not, an appropriate LDC for the leak detection system would need to be established. The famous UK PARLOC database was used for the calculation of pipeline failure rates, and the software POSVCM from MMS was used for oil spill simulations. QRA results revealed that the installation of a statistically based leak detection system (LDS) can significantly reduce time to leak detection, thereby mitigating the consequences of leakage. A sound LDC has been defined based on QRA study results and comments from various LDS vendors to assist the emergency response team (ERT) to quickly identify and locate leakage and employ the most effective measures to contain damage.展开更多
Security is a nonfunctional information system attribute that plays a crucial role in wide sensor network application domains. Security risk can be quantified as the combination of the probability that a sensor networ...Security is a nonfunctional information system attribute that plays a crucial role in wide sensor network application domains. Security risk can be quantified as the combination of the probability that a sensor network system may fail and the evaluation of the severity of the damage caused by the failure. In this paper, we devise a methodology of Rough Outlier Detection (ROD) for the detection of security-based risk factor, which originates from violations of attack requirements (namely, attack risks). The methodology elaborates dimension reduction method to analyze the attack risk probability from high dimensional and nonlinear data set, and combines it with rough redundancy reduction and the distance measurement of kernel function which is obtained using the ROD. In this way, it is possible to determine the risky scenarios, and the analysis feedback can be used to improve the sensor network system design. We illustrate the methodology in the DARPA case set study using step-by-step approach and then prove that the method is effective in lowering the rate of false alarm.展开更多
Objective. So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solv...Objective. So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. Materials and methods. Based on the M. leprae gene coding groEL, 65kD and 16S rRNA, three polymerase chain reactions were developed by using plikaytis’, Woods’ and Pattyn’s procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose.Results and conclusion. For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practicl purpose, the woods’ PCR optimized by us ought to be chosen firstly.展开更多
Signal Detection Theory (SDT) offers an unparalleled deterministic set of decision variables necessary to formulate applied risks in transportation. SDT has distinct advantages over basic prediction models since the...Signal Detection Theory (SDT) offers an unparalleled deterministic set of decision variables necessary to formulate applied risks in transportation. SDT has distinct advantages over basic prediction models since the latter may not represent an entirely accurate analysis. Thresholds based on elements of stimulus (signal and noise) and response for: a Type I discrimination of response variable where decision outcomes and rates are computed for metacognition to discriminate a Type II of decision outcomes was set. We also adapted the classical Dijkstra's shortest path algorithm within a GIS environment using Avenue programming. Contours derived from LiDARwere used to set flood levels while satellite imagery corresponding to Red River of the North inundated (signal) areas were acquired amongst other spatial datasets. The signal information was further dichotomized using a binary yes-no model. Origin and destination points constrained within Fargo-Morehead were generated using a random point generator. From these points, trips were generated with some connected segments traversing through flooded areas. By analyzing False Alarm Rate (FAR) and Corrected Rejection (CRR) computation, we found out that, when Hit Rate (HR) and FAR are both low then there was an increased corresponding sensitivity. At 30-35 ft flood level, the values for FAR and HR was 0.97 and 0.91 respectively.When FAR〉HR, lower set flood levels offered numerous route choices. Corresponding routes with associated impedance can be classified for risk-averse drivers or risk-takers While the risk-averse avoid risky and unfavorable routes, the risk-taker optimizes at an adjustment factor of ω = 0.1 or ω = 0.2. An idealistic stage is achieved for a conservative, co, equal to 0.4 or 0.5, which indicates maximum achievement in terms of time gain and safety simultaneously. At ω = 0.0 the prevailing conditions can be considered unrealistic since they incorporate areas considered impassable with absolute resistance like segments with a "Road Closed" or "Detour" sign. The applicability of our approach can be used to design multi-level and multi-modal transportation systems involving risk.展开更多
Trend analysis and change point detection in a time series are frequent analysis tools.Change point detection is the identification of abrupt variation in the process behaviour due to natural or artificial changes,whe...Trend analysis and change point detection in a time series are frequent analysis tools.Change point detection is the identification of abrupt variation in the process behaviour due to natural or artificial changes,whereas trend can be defined as estimation of gradual departure from past norms.We analyze the time series data in the presence of trend,using Cox-Stuart methods together with the change point algorithms.We applied the methods to the nearsurface wind speed time series for Australia as an example.The trends in near-surface wind speeds for Australia have been investigated based upon our newly developed wind speed datasets,which were constructed by blending observational data collected at various heights using local surface roughness information.The trend in wind speed at 10 m is generally increasing while at 2 m it tends to be decreasing.Significance testing,change point analysis and manual inspection of records indicate several factors may be contributing to the discrepancy,such as systematic biases accompanying instrument changes,random data errors(e.g.accumulation day error)and data sampling issues.Homogenization technique and multiple-period trend analysis based upon change point detections have thus been employed to clarify the source of the inconsistencies in wind speed trends.展开更多
The altimeter normalized radar cross section(NRCS) has been used to retrieve the sea surface wind speed for decades, and more than a dozen of wind speed retrieval algorithms have been proposed. Despite the continuing ...The altimeter normalized radar cross section(NRCS) has been used to retrieve the sea surface wind speed for decades, and more than a dozen of wind speed retrieval algorithms have been proposed. Despite the continuing efforts to improve the wind speed measurements, a bias dependence on wave state persists in all wind algorithms. On the basis of recent evidence that short waves are essentially modulated by local winds and much less affected by wave state, we proposed a physics-based approach to retrieve the wind speed from the dual-frequency difference in terms of the mean square slope of short waves. A collocated dataset of coincident altimeter/buoy measurements were used to develop and validate the approach. Validation against buoy measurements indicates that the approach is almost unbiased and has an overall root mean square error of 1.24 m s-1, which is 5.3% lower than the single-parameter algorithm in operational use(Witter and Chelton, 1991) and 2.4% lower than another dual-frequency approach(Chen et al., 2002). Furthermore, the results indicate that the new approach significantly improves the wave-dependent bias compared to the single-parameter algorithm. The capacity of altimeter to retrieve sea surface wind speed appears to be limited for the case of winds below 3 m s-1. The validity of the approach at high winds needs to be further examined in the future study.展开更多
基金Supported by General Program of Science and Technology Plan of Beijing Municipal Commission of Educational(KM201410020007)~~
文摘[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 mm × 150 mm,5 μm).The mobile phase was acetonitrile-water(35∶65).The ELSD evaporator tube temperature was 65 ℃.N2 was used as the carrier gas(pressure,30 psi).[Result] When the content of Astragaloside IV ranged from 0.5 to 5.0 μg,the Astragaloside IV content showed a good linear relationship with peak area(r=0.999,n=6).The average recovery was 96.36%,and the RSD was 2.46%.[Conclusion] This method is accurate and reliable,and can be applied in the quality control of Yupingfeng oral solution.
基金Supported by Health Technology Fund of Yunnan Province, China,No.2010NS066
文摘AIM: To investigate the expression of Popeye domain containing 3 (Popdc3) and its correlation with clinicopathological features and prognosis of gastric cancer.METHODS: The method of immunohistochemistry was used to investigate the expression of Popdc3 in 306 cases of human gastric cancer and 84 noncancerous gastric tissues. Simultaneously, the relationship between Popdc3 expression and the survival of the patients was retrospectively analyzed.RESULTS: Popdc3 was detected in 72 (85.71%) of 84 human nontumor mucosa. High expression of Popdc3protein was detected in 78 (25.49%) of 306 human gastric cancer cases, and low expression was detected in 228 (74.51%). Low expression of Popdc3 correlated with depth of invasion (P 〈 0.0001), regional lymph nodes (P 〈 0.0001) and distant metastasis (P =0.02), and tumor, nodes, metastasis (TNM) stages (P 〈 0.0001). On multivariate analysis, only the patient's gender, regional lymph node metastasis, distant metastasis, TNM stages, and the expression of Popdc3 were independent prognostic factors in patients with gastric cancer. The Kaplan-Meier plot showed that low Popdc3 expression had a much more significant effect on the survival of those patients with early-stage tumors X^2 = 104.741, P 〈 0.0001), with a 〉 51.9% reduction in the three-year survival compared with high Popdc3 expression. In late stages, the difference was also significant X^2 = 5.930, P = 0.015), with a 32.6% reduction in the three-year survival.CONCLUSION: Reduced expression of Popdc3 may play a significant role in the carcinogenesis and progression of gastric cancer. Popdc3 may be an independent prognostic factor.
文摘A quantitative risk assessment (QRA) based on leak detection criteria (LDC) for the design of a proposed subsea oil export pipeline is presented in this paper. The objective of this QRA/LDC study was to determine if current leak detection methodologies were sufficient, based on QRA results, while excluding the use of statistical leak detection; if not, an appropriate LDC for the leak detection system would need to be established. The famous UK PARLOC database was used for the calculation of pipeline failure rates, and the software POSVCM from MMS was used for oil spill simulations. QRA results revealed that the installation of a statistically based leak detection system (LDS) can significantly reduce time to leak detection, thereby mitigating the consequences of leakage. A sound LDC has been defined based on QRA study results and comments from various LDS vendors to assist the emergency response team (ERT) to quickly identify and locate leakage and employ the most effective measures to contain damage.
基金the Jiangsu 973 Scientific Project,the National Natural Science Foundation of China,the Jiangsu Natural Science Foundation,the Aerospace Innovation Fund,the Lianyungang Science & Technology Project
文摘Security is a nonfunctional information system attribute that plays a crucial role in wide sensor network application domains. Security risk can be quantified as the combination of the probability that a sensor network system may fail and the evaluation of the severity of the damage caused by the failure. In this paper, we devise a methodology of Rough Outlier Detection (ROD) for the detection of security-based risk factor, which originates from violations of attack requirements (namely, attack risks). The methodology elaborates dimension reduction method to analyze the attack risk probability from high dimensional and nonlinear data set, and combines it with rough redundancy reduction and the distance measurement of kernel function which is obtained using the ROD. In this way, it is possible to determine the risky scenarios, and the analysis feedback can be used to improve the sensor network system design. We illustrate the methodology in the DARPA case set study using step-by-step approach and then prove that the method is effective in lowering the rate of false alarm.
文摘Objective. So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. Materials and methods. Based on the M. leprae gene coding groEL, 65kD and 16S rRNA, three polymerase chain reactions were developed by using plikaytis’, Woods’ and Pattyn’s procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose.Results and conclusion. For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practicl purpose, the woods’ PCR optimized by us ought to be chosen firstly.
文摘Signal Detection Theory (SDT) offers an unparalleled deterministic set of decision variables necessary to formulate applied risks in transportation. SDT has distinct advantages over basic prediction models since the latter may not represent an entirely accurate analysis. Thresholds based on elements of stimulus (signal and noise) and response for: a Type I discrimination of response variable where decision outcomes and rates are computed for metacognition to discriminate a Type II of decision outcomes was set. We also adapted the classical Dijkstra's shortest path algorithm within a GIS environment using Avenue programming. Contours derived from LiDARwere used to set flood levels while satellite imagery corresponding to Red River of the North inundated (signal) areas were acquired amongst other spatial datasets. The signal information was further dichotomized using a binary yes-no model. Origin and destination points constrained within Fargo-Morehead were generated using a random point generator. From these points, trips were generated with some connected segments traversing through flooded areas. By analyzing False Alarm Rate (FAR) and Corrected Rejection (CRR) computation, we found out that, when Hit Rate (HR) and FAR are both low then there was an increased corresponding sensitivity. At 30-35 ft flood level, the values for FAR and HR was 0.97 and 0.91 respectively.When FAR〉HR, lower set flood levels offered numerous route choices. Corresponding routes with associated impedance can be classified for risk-averse drivers or risk-takers While the risk-averse avoid risky and unfavorable routes, the risk-taker optimizes at an adjustment factor of ω = 0.1 or ω = 0.2. An idealistic stage is achieved for a conservative, co, equal to 0.4 or 0.5, which indicates maximum achievement in terms of time gain and safety simultaneously. At ω = 0.0 the prevailing conditions can be considered unrealistic since they incorporate areas considered impassable with absolute resistance like segments with a "Road Closed" or "Detour" sign. The applicability of our approach can be used to design multi-level and multi-modal transportation systems involving risk.
文摘Trend analysis and change point detection in a time series are frequent analysis tools.Change point detection is the identification of abrupt variation in the process behaviour due to natural or artificial changes,whereas trend can be defined as estimation of gradual departure from past norms.We analyze the time series data in the presence of trend,using Cox-Stuart methods together with the change point algorithms.We applied the methods to the nearsurface wind speed time series for Australia as an example.The trends in near-surface wind speeds for Australia have been investigated based upon our newly developed wind speed datasets,which were constructed by blending observational data collected at various heights using local surface roughness information.The trend in wind speed at 10 m is generally increasing while at 2 m it tends to be decreasing.Significance testing,change point analysis and manual inspection of records indicate several factors may be contributing to the discrepancy,such as systematic biases accompanying instrument changes,random data errors(e.g.accumulation day error)and data sampling issues.Homogenization technique and multiple-period trend analysis based upon change point detections have thus been employed to clarify the source of the inconsistencies in wind speed trends.
基金supported by the National High Technology Research and Development Program of China (2013 AA09A505)
文摘The altimeter normalized radar cross section(NRCS) has been used to retrieve the sea surface wind speed for decades, and more than a dozen of wind speed retrieval algorithms have been proposed. Despite the continuing efforts to improve the wind speed measurements, a bias dependence on wave state persists in all wind algorithms. On the basis of recent evidence that short waves are essentially modulated by local winds and much less affected by wave state, we proposed a physics-based approach to retrieve the wind speed from the dual-frequency difference in terms of the mean square slope of short waves. A collocated dataset of coincident altimeter/buoy measurements were used to develop and validate the approach. Validation against buoy measurements indicates that the approach is almost unbiased and has an overall root mean square error of 1.24 m s-1, which is 5.3% lower than the single-parameter algorithm in operational use(Witter and Chelton, 1991) and 2.4% lower than another dual-frequency approach(Chen et al., 2002). Furthermore, the results indicate that the new approach significantly improves the wave-dependent bias compared to the single-parameter algorithm. The capacity of altimeter to retrieve sea surface wind speed appears to be limited for the case of winds below 3 m s-1. The validity of the approach at high winds needs to be further examined in the future study.