目的建立骨骼风痛片中鸡血藤的高效液相特征图谱分析方法。方法采用HPLC-UV法,以芒柄花素为参照物,采用色谱柱GraceSmart RP 18(5μm,4.6mm×250mm),以乙腈-0.1%磷酸(34:66)为流动相,柱温40℃,流速0.8 mL/min。结果鸡血藤有6个共有...目的建立骨骼风痛片中鸡血藤的高效液相特征图谱分析方法。方法采用HPLC-UV法,以芒柄花素为参照物,采用色谱柱GraceSmart RP 18(5μm,4.6mm×250mm),以乙腈-0.1%磷酸(34:66)为流动相,柱温40℃,流速0.8 mL/min。结果鸡血藤有6个共有峰,10批不同来源的鸡血藤药材相似度在90%以上,确定了骨骼风痛片成品中3个特征峰归属于鸡血藤。结论该方法简便、快速,可为骨骼风痛片的质量提供更为科学、准确的控制。展开更多
目的建立RP-HPLC-DAD法用于检测风湿寒痛片中延胡索乙素、羌活醇和桂皮醛3种活性成分含量。方法采用Capcell Pak UG C_(18)色谱柱分离,柱温设定为30℃、流速设定为1.0 mL·min^(-1),流动相A为乙腈,流动相B为0.5%磷酸溶液,梯度洗脱,...目的建立RP-HPLC-DAD法用于检测风湿寒痛片中延胡索乙素、羌活醇和桂皮醛3种活性成分含量。方法采用Capcell Pak UG C_(18)色谱柱分离,柱温设定为30℃、流速设定为1.0 mL·min^(-1),流动相A为乙腈,流动相B为0.5%磷酸溶液,梯度洗脱,检测波长:280 nm(延胡索乙素)、310 nm(羌活醇)、290 nm桂皮醛;进样体积10μL,采用RP-HPLC行定量检测。结果延胡索乙素、羌活醇和桂皮醛分别在质量浓度0.4568-45.6800、0.0585-5.8500、0.00988-0.98000μg·mL^(-1)内具有良好的线性,平均回收率分别为99.39%、100.27%和99.13%;仪器精密度和方法重复性均在5.0%以内。结论实验建立的RP-HPLC-DAD法具有简单、快速、准确等特点,可以用于风湿寒痛片产品的质量控制。展开更多
To verify the therapeutic effects of the method of softening and lubricating the joints,and calming the endogenous wind in case of rheumatoid arthritis (RA) with the syndrome of channel blockage due to yin deficiency,...To verify the therapeutic effects of the method of softening and lubricating the joints,and calming the endogenous wind in case of rheumatoid arthritis (RA) with the syndrome of channel blockage due to yin deficiency,60 RA patients with the syndrome of channel blockage due to yin deficiency were randomly divided into a treatment group (40 cases) and a control group (20 cases) and treated respectively by the above method for the former and with Zheng Qing Feng Tong Ning Tablets (正清风痛宁片) for the latter. The result turned out to be that the effect in the treatment group was very satisfying.The treatment group obtained a better result in the accumulated points of syndrome and RA,morning rigidity of the joints,grip strength,20m walking time and erythrocyte sedimentation rate (ESR) (P<0.01 or P<0.05).The above indicates that channel blockage due to yin deficiency is an important pathogenesis of RA,and calming the endogenous wind is a method of choice for treating RA.展开更多
文摘目的建立骨骼风痛片中鸡血藤的高效液相特征图谱分析方法。方法采用HPLC-UV法,以芒柄花素为参照物,采用色谱柱GraceSmart RP 18(5μm,4.6mm×250mm),以乙腈-0.1%磷酸(34:66)为流动相,柱温40℃,流速0.8 mL/min。结果鸡血藤有6个共有峰,10批不同来源的鸡血藤药材相似度在90%以上,确定了骨骼风痛片成品中3个特征峰归属于鸡血藤。结论该方法简便、快速,可为骨骼风痛片的质量提供更为科学、准确的控制。
文摘目的建立RP-HPLC-DAD法用于检测风湿寒痛片中延胡索乙素、羌活醇和桂皮醛3种活性成分含量。方法采用Capcell Pak UG C_(18)色谱柱分离,柱温设定为30℃、流速设定为1.0 mL·min^(-1),流动相A为乙腈,流动相B为0.5%磷酸溶液,梯度洗脱,检测波长:280 nm(延胡索乙素)、310 nm(羌活醇)、290 nm桂皮醛;进样体积10μL,采用RP-HPLC行定量检测。结果延胡索乙素、羌活醇和桂皮醛分别在质量浓度0.4568-45.6800、0.0585-5.8500、0.00988-0.98000μg·mL^(-1)内具有良好的线性,平均回收率分别为99.39%、100.27%和99.13%;仪器精密度和方法重复性均在5.0%以内。结论实验建立的RP-HPLC-DAD法具有简单、快速、准确等特点,可以用于风湿寒痛片产品的质量控制。
文摘To verify the therapeutic effects of the method of softening and lubricating the joints,and calming the endogenous wind in case of rheumatoid arthritis (RA) with the syndrome of channel blockage due to yin deficiency,60 RA patients with the syndrome of channel blockage due to yin deficiency were randomly divided into a treatment group (40 cases) and a control group (20 cases) and treated respectively by the above method for the former and with Zheng Qing Feng Tong Ning Tablets (正清风痛宁片) for the latter. The result turned out to be that the effect in the treatment group was very satisfying.The treatment group obtained a better result in the accumulated points of syndrome and RA,morning rigidity of the joints,grip strength,20m walking time and erythrocyte sedimentation rate (ESR) (P<0.01 or P<0.05).The above indicates that channel blockage due to yin deficiency is an important pathogenesis of RA,and calming the endogenous wind is a method of choice for treating RA.
